Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Immunoadjuvant Activity of Korean Mistletoe Lectin B-chain

한국산 겨우살이 Lectin B-chain의 면역증강 효과

  • Her, Sun-Mi (School of Life Sciences, Handong Global University) ;
  • An, Hyo-Sun (School of Life Sciences, Handong Global University) ;
  • Kim, Kyu-Dae (School of Life Sciences, Handong Global University) ;
  • Kim, Young-Hoon (School of Life Sciences, Handong Global University) ;
  • Kim, In-Bo (School of Life Sciences, Handong Global University) ;
  • Yoon, Taek-Joon (Department of Food and Nutrition, Yuhan University) ;
  • Kim, Jong-Bae (School of Life Sciences, Handong Global University)
  • 허선미 (한동대학교 생명과학부 생명과학과) ;
  • 안효선 (한동대학교 생명과학부 생명과학과) ;
  • 김규대 (한동대학교 생명과학부 생명과학과) ;
  • 김영훈 (한동대학교 생명과학부 생명과학과) ;
  • 김인보 (한동대학교 생명과학부 생명과학과) ;
  • 윤택준 (유한대학교 식품영양학과) ;
  • 김종배 (한동대학교 생명과학부 생명과학과)
  • Received : 2011.06.02
  • Accepted : 2011.08.11
  • Published : 2011.09.30
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Abstract

Korean mistletoe Lectin (KML-C) is composed of A and B sub-chain. B chain binds to carbohydrates on cell surface and A chain hinders translation and induces an apoptosis as a RIP (ribosome inactivating protein). KML-C has very strong biological activities, it has seriously limits to use as a cancer therapy or adjuvant because of its toxicity to normal cells. This study is therefore conducted to see if B chain of KML-C might have immunological activity, especially adjuvant activities with less toxicity. We isolated B chain from KML-C using the lactose affinity chromatography, and examined their immunoadjuvant activity. The isolated B-chain did not show any cytotoxicity against tumor cell, RAW264.7, and P388D1 while KML-C had a very strong toxicity. This non-toxic effect was observed also by in-vivo study. Both humoral and cellular immunities were observed ; the antibody titer was increased when the mice were immunized with B-chain used as adjuvant like Freund's adjuvant, indicating that B chain of mistletoe lectin alone might be used for adjuvant; it also increased DTH in cellular immunity. These results suggest that B-chain of KML-C might be used for adjuvant used for the production of antibody or vaccine with less toxicity.

Keywords

References

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