Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
권호 표지
DOI QR코드

DOI QR Code

Association between the Polymorphism of the Fatty acid binding protein 5 (FABP5) Gene within the BTA 14 QTL Region and Carcass/Meat Quality Traits in Hanwoo

한우 14번 염색체 QTL 영역내 Fatty acid binding protein 5 유전자의 다형성과 도체 및 육질 형질과의 관련성 분석

  • Received : 2011.03.18
  • Accepted : 2011.05.19
  • Published : 2011.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this study was to evaluate the association between economic traits of Korean cattle (Hanwoo) and genetic variation in fatty acid binding protein 5 (FABP5) gene within QTL region of carcass weight and marbling score traits on BTA 14. We sequenced for detection of single nucleotide polymorphism (SNP) with 24 unrelated Hanwoo samples and identified four SNPs (-1141A>G, 949A>G, 969A>G and 1085C>G). Relationship between the genotypes of 583 Hanwoo individuals by PCR-RFLP and economic traits were analyzed by the mixed regression model implemented in the ASReml program. As the result of statistical analysis, SNP1 (-1141A>G) showed significant effect (p<0.003) on marbling score (MS) and SNP2 (949A>G) showed significant effect (p<0.034) on eye muscle area (EMA). Further studies are required to validate the significant SNPs in a bigger population, but the SNPs (-1141A>G and 949A>G) of FABP5 could be a genetic marker to estimate molecular breeding value (MEBV) for carcass traits in Hanwoo.

본 연구는 한우에서 소 염색체 14번(BTA14)에서 근내지방도 및 도체중과 관련성이 보고된 QTL영역(48-58cM) 내의 FABP5 유전자를 대상으로 SNP를 발굴하고 도체형질과의 관련성 분석을 위하여 수행하였다. PCR 및 염기서열결정법을 통해 FABP5 유전자내 4개의 SNP (-1141A>G, 949A>G, 969A>G, 1085C>G)를 발굴하였고, 이중 promoter 영역에 위치하는 SNP의 경우 미 보고된 신규 SNP로 확인되었다. 발굴된 4개의 SNP를 대상으로 표현형 기록치를 보유한 후대검정후 583두에 대하여 유전자형 분석 및 관련성 분석을 수행하였다. 분석 결과 4개의 SNP중 SNP1(-1141A>G)은 근내지방도에 있어서 G유전자형이 A유전자형에 비해서 근내지방도가 2.2 정도 높았고, SNP2 (949A>G)는 배최장근 단면적에 있어서 G유전자형이 A유전자형보다 배장근단면적에 있어서 3 $cm^2$ 만큼 높은 효과를 보였다. 본 결과에 대해 추후 지속적인 연구가 요구되어지며, FABP5의 SNPs은 한우의 도체 및 육질 관련 형질을 위한 유전자 마커로서 활용 가능할 수 있을 것으로 사료된다.

Keywords

References

  1. Ajit, C., Suvannasankha, A., Zaeri, N. and Munoz, S. J. 2003. Terbinafine-associated hepatotoxicity. Am. J. Med. Sci. 325(5): 292-295. https://doi.org/10.1097/00000441-200305000-00008
  2. Casas, E., Keele, J. W., Shackelford, S. D., Koohmaraie, M. and Stone, R. T. 2003. Identification of quantitative trait loci for growth and carcass composition in cattle. Anim. Genet. 35:2-6.
  3. Cheong, H. S., Yoon, D., Kim, L. H., Park, B. L., Lee, H. W., Han, C. S., Kim, E. M., Cho, H., Chung, E. R., Cheong, I. J. and Shin, H. D. 2007. Titin-cap (TCAP) polymorphisms associated with marbling score of beef. Meat Sci. 77:257-263. https://doi.org/10.1016/j.meatsci.2007.03.014
  4. Cheong, H. S., Yoon, D. H., Park, B. L., Kim, L. H., Bae, J. S., Sohg Namgoong, Hae Won Lee, Han, C. S., Kim, J. O., Cheong, I. C. and Shin, H. D. 2008. A single nucleotide polymorphism in CAPN1 associated with marbling score in Korean cattle. BMC Genetics. 9:33.
  5. Cheong, H.S, Yoon, DH, Bae, JS, Kim, LH, Kim, EM, Kim, JO, Hong, J, Kim, N. and Shin, HD. 2009. ADFP promoter polymorphism associated with marbling score in Korean cattle. BMB reports. 42(8):529-34. https://doi.org/10.5483/BMBRep.2009.42.8.529
  6. Cho, S., Park, T. S., Yoon, D., Cheong, H. S., Namgoong, S., Park, B. L., Lee, H. W., Han, C. S., Kim, E. M., Cheong, I. C., Kim, H. and Shin, H. D. 2008. Identification of genetic polymorphisms in FABP3 and FABP4 and putative association with back fat thickness in Korean native cattle. BMB reports. 41:29-34. https://doi.org/10.5483/BMBRep.2008.41.1.029
  7. Darvasi, A. and Soller, M. 1994. Selective DNA pooling for determination of linkage between a molecular marker and a quantitative trait locus. Genetics. 138:1365-1373.
  8. Estelle, T., Perez-Enciso, M., Mercade, A., Varona, L., Alves, E., Sanchez, A. and Folch, J. M. 2006. Characteization of the porcine FABP5 gene and its association with the FAT1 QTL in an Iberian by Landrace cross. Anim. Genet. 37(6):589-591. https://doi.org/10.1111/j.1365-2052.2006.01535.x
  9. Gilmour, A. R., Gogel, B. J., Cullis, B. R. and Thompson, R. 2006. ASREML User Guide Release 2.0 VSN International Ltd., Hemel Hempstead, HP1 1ES, UK.
  10. Gutierrez-Gil, B., Wiener, P., Nute, G. R., Burton, D., Gill, J. L., Wood, J. D. and Williams, J. L. 2007. Detection of quantitative trait loci for meat quality traits in cattle. Anim. Genet. 39:51-61.
  11. Hollung, K., Veiseth, E., Jia, X., Færgestad, E. M. and Hildrum, K. I. 2007. Application of proteomics to understand the molecular mechanism behind meat quality. Meat Sci. 77:97-104. https://doi.org/10.1016/j.meatsci.2007.03.018
  12. Kawase, T., Akatsuka, Y., Torikai, H., Morishima, S., Oka, A., Tsujimura, A., Miyazaki, M., Tsujimura, K., Miyamura, K., Ogawa, S., Inoko, H., Morishima, Y., Kodera, Y., Kuzushima, K. and Takahashi, T. 2007. Alternative splicing due to intronic SNP in HMSD generates a novel minor histocompatibility antigen. Blood. 110:1055-1063. https://doi.org/10.1182/blood-2007-02-075911
  13. Kim, B. S., Kim, N. K., Lee, S. H., Cho, Y. M., Heo, K. N., Park, E. W. Yang, B. K. and Yoon, D. 2011. Association study between the polymorphisms of Exostosin-1 gene and economic traits in Hanwoo. 53(1):7-13 https://doi.org/10.5187/JAST.2011.53.1.7
  14. Kong, H. S., Oh, J. D., Lee, J. H., Yoon, D. H., Choi, Y. H., Cho, B. W., Lee, H. K. and Jeon, G. J. 2007. Association of sequence variations in DGAT 1 gene with economic traits in Hanwoo (Korea cattle). Asian-Aust. Anim. Sci. 20:817-820.
  15. Krieg P., Feil S., Furstenberger, G. and Bowden, G. T. 1993. Tumor-specific overexpression of a novel keratinocyte lipidbinding protein: identification and characterization of a cloned sequence activated during multistage carcinogenesis in mouse skin. Biol. Chem. 268:17362-17369.
  16. Lande, R. and Thompson, R. 1990. Efficiency of marker assisted selection in the improvement of quantitative traits. Genetics. 124:743-756.
  17. Lee, H. J., Lee, S. H., Cho, Y. M., Yoon, H. B., Jeon, B. K., Oh, S. J., Kwon, M. S. and Yoon, D. H. 2004. Association Between the Polymorphism on Intron 5 of the Lipoprotein Lipase Gene and Carcass Traits in Hanwoo (Korean cattle). Kor. J. Anim. Sci. Technol. 46:947-956. https://doi.org/10.5187/JAST.2004.46.6.947
  18. Lee, S. H., Park, E. W., Cho, Y. M., Kim, S. K., Lee J. H., Jeon, J.T., Lee, C. S., Im, S. K., Oh. S. J, Thompson, J. M. and Yoon D. 2007. Identification of differentially expressed genes related to intramuscular fat development in the early and late fattening stages of hanwoo steers. Biochem. Mol. Biol. 40(5):757-764. https://doi.org/10.5483/BMBRep.2007.40.5.757
  19. Lee, S. H., van der Werf, J. H. J., Lee, S. H., Park, E. W., Oh, S. J., Gibson, J. P. and Thompson, J. M. 2010. Genetic polymorphisms of the bovine Fatty acid binding protein 4 gene are significantly associated with marbling and carcass weight in Hanwoo (Korean Cattle). Anim. Genet. 41(4):442-444.
  20. Liu, Z., Sun, H. X., Zhang, Y. W., Li, Y. F., Zuo, J., Meng, Y. and Fang, F. D. 2004. Effect of SNPs in protein kinase Cz gene on gene expression in the reporter gene detection system. World J. Gastroenterol. 10:2357-2360.
  21. Luis, H., Gutierrez, C., Christian, L., Carsten, H., Martin, R., Thorsten, H., Heinz, R., Friedrich, S. and Christian, L. 2002. Solution structure and backbone dynamics of human epidermaltype fatty acid-binding protein (E-FABP). Biochem. 364:725-737. https://doi.org/10.1042/BJ20020039
  22. Maeda, K., Uysal, K. T., Makowski, L., Gorgün, C. Z., Atsumi, G., Parker, R. A., Brüning, J., Hertzel, A. V., Bernlohr, D. A. and Hotamisligil, G. S. 2003. Role of the fatty acid binding protein mal1 in obesity and insulin resistance. Diabetes. 52:300-307. https://doi.org/10.2337/diabetes.52.2.300
  23. Marques, E., Nkrumah, J. D., Sherman, E. L. and Moore, S. S. 2009. Polymorphisms in positional candidate genes on BTA14 and BTA26 affect carcass quality in beef cattle. J. Anim. Sci. 87:2475-2484. https://doi.org/10.2527/jas.2008-1456
  24. Miller, S. A., Dykes, D. D. and Polesky, H. F. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucl. Acids Res. 16:1215. https://doi.org/10.1093/nar/16.3.1215
  25. Shin, S. C. and Chung, E. R. 2007. Association of SNP Marker in the Thyroglobulin Gene with Carcass and Meat Quality Traits in Korean Cattle. Asian-Aust. J. Anim. Sci. 20(2):172-177.
  26. Wang, Y. H., Reverter, A., Tan, S. H., Jager, N. D., Eang, R., McWilliam, S. M., Cafe, L.M., Greenwood, P. L. and Lehnert, S. A. 2009. Gene expression patterns during intramuscular fat development in cattle. J. Ainm. Sci. 87:119-130. https://doi.org/10.2527/jas.2008-1082
  27. Wibowo, T. A., Gaskins, C. T., Newberry, R. C., Thorgaard G. H., Michal, J. J. and Jiang, Z. 2008. Genome Assembly Anchored QTL Map of Bovine Chromosome 14. Int. J. Biol. Sci. 4:406- 414. https://doi.org/10.3923/ijb.2008.406.420

Cited by

  1. Application of DNA marker related with marbling score in Hanwoo cow vol.27, pp.3, 2016, https://doi.org/10.7465/jkdi.2016.27.3.733
  2. Validation of DNA Markers for Carcass Traits with Commercial Hanwoo Population vol.48, pp.2, 2014, https://doi.org/10.14397/jals.2014.48.2.133