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아까시 꽃 추출물의 항산화 활성 및 DNA 손상 억제 효과

Antioxidant Activity and DNA Damage Protective Effect of a Robinia pseudoacacia L. Flower Extract

  • 김수정 (경남대학교 식품생명학과) ;
  • 서고운 (경남대학교 식품생명학과) ;
  • 서보영 (경남대학교 식품영양학과) ;
  • 박은주 (경남대학교 식품영양학과) ;
  • 이승철 (경남대학교 식품생명학과)
  • Kim, Su-Jung (Dept. of Food Science and Biotechnology, Kyungnam University) ;
  • Seo, Go-Un (Dept. of Food Science and Biotechnology, Kyungnam University) ;
  • Seo, Bo-Young (Dept. of Food and Nutrition, Kyungnam University) ;
  • Park, Eun-Ju (Dept. of Food and Nutrition, Kyungnam University) ;
  • Lee, Seung-Cheol (Dept. of Food Science and Biotechnology, Kyungnam University)
  • 발행 : 2011.08.30

초록

아까시 꽃 10 g에 200 mL의 두 가지 용매(물, 70% 에탄올)를 각각 가하여 추출한 다음, 농축하여 각각의 용매별 추출물을 얻었다. 이 용매별 추출물을 이용하여 아까시 꽃의 항산화 활성을 조사하였다. 그 결과, 총 페놀 함량은 물 추출물이 9.07 mg GAE/g로 70% 에탄올 추출물보다 높았고, 총 플라보노이드 함량은 70% 에탄올 추출물에서 0.04 mg CE/g, 물 추출물은 0.03 mg CE/g으로 나타났다. DPPH 라디칼 소거능은 대부분 70% 에탄올 추출물이 물 추출물 보다 더 높은 활성을 나타내었고, 환원력의 경우에는 물 추출물의 환원력은 1,000 mg/mL의 농도에서 0.438로 가장 높았으며 70% 에탄올보다 더 높은 경향을 보였다. Comet assay를 이용한 항유전독성의 효과 분석 결과, 물 추출물에서는 모든 농도에서 유의적인 DNA 손상 억제력을 보였으며, 에탄올 추출물에서는 고농도(10, 50 ${\mu}g/ml$)에서 DNA 손상이 감소되는 것을 알 수 있었다.

The antioxidant properties of Robinia pseudoacacia L. water and 70% ethanol extracts were evaluated by determining total phenolic content (TPC), DPPH radical scavenging activity (RSA), and reducing power (RP). The water extract showed higher TPC (9.07 mg/g gallic acid equivalents) and RP than those of ethanol extract, whereas ethanol extract had greater DPPH RSA. The R. pseudoacacia L. extracts also showed antigenotoxic effects for 200 ${\mu}M$ $H_2O_2$-induced DNA damage in human leukocytes. The 200 ${\mu}M$ $H_2O_2$-induced DNA damage decreased following treatment with the water extract. Reductions in DNA damage with 50 ${\mu}g/ml$ of the water and ethanol extracts were 46.5 and 32.4%, respectively.

키워드

참고문헌

  1. Andarwulan N, Batari R, Sandrasari DA, Bolling B, Wijaya H. 2010. Flavonoid content and antioxidant activity of vegetables from Indonesia. Food Chem 121:1231-1235 https://doi.org/10.1016/j.foodchem.2010.01.033
  2. Balasundram N, Sundram K, Samman S. 2006. Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses. Food Chem 99:191-203 https://doi.org/10.1016/j.foodchem.2005.07.042
  3. Buxiang S, Fukuhara M. 1997. Effects of co-administration of butylated hydroxytoluene, butylated hydroxyanisole and flavonoide on the activation of mutagens and drug-metabolizing enzymes in mice. Toxicology 122(1-2):61-72 https://doi.org/10.1016/S0300-483X(97)00078-4
  4. Caillet S, Yu H, Lessard S, Lamoureux G, Ajdukovic D, Lacroix M. 2007. Fenton reaction applied for screening natural antioxidants. Food Chem 100(2):542-552 https://doi.org/10.1016/j.foodchem.2005.10.009
  5. Choi DH, Lee HJ, Lee SS, Kim YG, Kang HY. 2002. Studies on biological activity of wood extractives. Mokchae Konghak 30(4):51-57
  6. Decker EA. 1995. The role of phenolics, conjugated linoleic acid, carnosine and pyrroloquinoline quinone as nonessential dietary antioxidants. Nutr Rev 53(3):49-58
  7. Gutfinger T. 1981. Polyphenols in olive oil. J Am Oil Chem Soc 58(11): 966-968. https://doi.org/10.1007/BF02659771
  8. Jang IC, Park JH, Park E, Park HR, Lee SC. 2008. Antioxidative and antigenotoxic activity of extracts from cosmos(Cosmos bipinnatus) flowers. Plant Foods Hum Nutr 63(4):205-210 https://doi.org/10.1007/s11130-008-0086-8
  9. Jeon GI, Yoon MY, Park HR, Lee SC, Park E. 2009. Neuroprotective activity of Viola mandshurica extracts on hydrogen peroxide-induced DNA damage and cell death in PC12 cells. Ann N Y Acad Sci 1171:576-82 https://doi.org/10.1111/j.1749-6632.2009.04889.x
  10. Jeong SM, Kim SY, Park HR, Lee SC. 2004. Effect of far-infrared radiation on the activity of extracts from Citrus unshiu peels. J Korean Soc Food Sci Nutr 33(9):1580-1583 https://doi.org/10.3746/jkfn.2004.33.9.1580
  11. Joung YM, Park SJ, Lee KY, Lee JY, Suh JK, Hwang SY, Park KE, Kang MH. 2007. Antioxidative and antimicrobial activities of Lilium species extracts prepared from different aerial parts. Korean J Food Sci Technol 39(4):452-457
  12. Jung ES, Park E, Park HR, Lee SC. 2008. Antioxidant activities of extracts from parts of Styela clava. J Korean Soc Food Sci Nutr 37(12):1674-1678 https://doi.org/10.3746/jkfn.2008.37.12.1674
  13. Kwon JH, Byun MW, Kim YH. 1995. Chemical composition of acacia flower (Robinia pseudo-acacia). J Korean Soc Food Sci Technol 27(5):789-793
  14. Kwon YS, Ban WS, Kim CM. 2000. The chemical constituents of the root cortex of Robinia pseudo-acacia. Yakhak Hoeji 44(5):411-415
  15. Larson RA. 1988. The antioxidants of higher plants. Phytochemistry 27(4):969-978 https://doi.org/10.1016/0031-9422(88)80254-1
  16. Lee BB, Chun JH, Lee SH, Park HR, Kim JM, Park EJ, Lee SC. 2007a. Antioxidative and antigenotoxic activity of extracts from Rhododendron mucromulatum Turcz. flowers. J Korean Soc Food Sci Nutr 36(12):1628-1632 https://doi.org/10.3746/jkfn.2007.36.12.1628
  17. Lee BB, Cha MR, Kim SY, Park E, Park HR, Lee SC. 2007b. Antioxidative and anticancer activity of extracts of cherry (Prunus serrulata var. spontanea) blossoms. Plant Foods Hum Nutr 62(2):79-84 https://doi.org/10.1007/s11130-007-0045-9
  18. Lee BB, Park SR, Han CS, Han DY, Park EJ, Park HR, Lee SC. 2008. Antioxidant activity and inhibition activity against $\alpha$ -amylase and $\alpha$-glucosidase of Viola mandshurica extracts. Korean Soc Food Sci Nutr 37(4):405-409 https://doi.org/10.3746/jkfn.2008.37.4.405
  19. Lee JM, Son ES, Oh SS, Han DS. 2001. Contents of total flavonoid and biological activities of edible plants. Korean J Dietary Culture 16(5):504-514
  20. Lee JM, Chung H, Chang PS, Lee JH. 2007. Development of a methods predicting the oxidative stability of edible oils using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Food Chem 103(2):662-669 https://doi.org/10.1016/j.foodchem.2006.07.052
  21. Lee SY, Hwang EJ, Kim GH, Choi YB, Lim CY, Kim SM. 2005. Antifungal and antioxidant activities of extracts from leaves and flowers of Camellia japonica L. Korean J Medicinal Crop Sci 13(3):93-100
  22. Nam SH, Chang SM, Kang MY. 2003. Varietal difference in antioxidative activity of ethanolic extracts from colored rice bran. J Korean Agric Chem Biotechnol 46(1):16-22
  23. Oh WG, Jang IC, Jeon GI, Park EJ, Park HR, Lee SC. 2008. Antioxidative activity of extracts from Wisteria floribunda flowers. J Korean Soc Food Sci Nutr 37(6):677-683 https://doi.org/10.3746/jkfn.2008.37.6.677
  24. Oyaizu M. 1996. Studies on product of browning reaction prepared from glucose amine. Jap J Nutr 44:307-315
  25. Park JS. 2008. The antioxidantive, antimicrobial activity and tyrosinase activity inhibitory effect of extracts from parts of Robinia pseudoacacia. MS Thesis. Soongsil University of Seoul, Korea. pp 22-23
  26. Park JS, Hwang IW, Zheng HZ, Kim SK, Chung SK. 2010. Determination of optimum hydrolysis conditions for flavonoid analysis in plant leaves. Korean J Food Preserv 17:261-266
  27. Petti S, Scully C. 2009. Polyphenols, oral health and disease: A review. J Dent 37:413-423 https://doi.org/10.1016/j.jdent.2009.02.003
  28. Price ML, Scoyoc SV, Butler GL. 1978. A critical evaluation of the vanillin reaction as an assay for tannin in sorghum grain. J Agric Food Chem 26(5):1214-1218 https://doi.org/10.1021/jf60219a031
  29. Seo HK, Jang SY, Kim HJ, Park HR, Park JH, Ahn GH, Lee SC. 2010. Antioxidant activity and neuroprotective effect of concentrates of commercial sweet persimmon wine. Korean J Food Cookery Sci 26(1):13-17
  30. Seo SB, Kim JH, Kim NM, Choi SY, Lee JS. 2002. Effect of acacia flower on the physiological functionality of Korean traditional rice wine. Kor J Microbiol Biotechnol 30(4):410-414
  31. Shin SR, Son YA, Won CY. 1993. Study on the components of lipids in acacia flower. J Food Sci Technol 5:61-67
  32. Surveswaran S, Cai YZ, Corke H, Sun M. 2007. Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants. Food Chem 102(3):938-953 https://doi.org/10.1016/j.foodchem.2006.06.033
  33. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. 2007. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39:44-84 https://doi.org/10.1016/j.biocel.2006.07.001

피인용 문헌

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