한국식품조리과학회지 (Korean journal of food and cookery science)

  • 제27권4호
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  • Pages.67-73
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  • 2011
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  • 2287-1780(pISSN)
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  • 2287-1772(eISSN)
한국식품조리과학회 (Korean Society of Food and Cookery Science)
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단감(Diospyros Kaki L.)껍질 추출물의 신경세포 보호 효과

Neuroprotective Effects of Extracts from Diospyros kaki L. Peel

  • 이미라 (경남대학교 식품생명학과) ;
  • 문성희 (경남대학교 식품생명학과) ;
  • 최애란 (경남대학교 식품생명학과) ;
  • 이승철 (경남대학교 식품생명학과) ;
  • 안광환 (경남농업기술원 단감연구소) ;
  • 박해룡 (경남대학교 식품생명학과)
  • Lee, Mi-Ra (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Moon, Seong-Hee (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Choi, Ae-Ran (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Lee, Seung-Cheol (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Ahn, Kwnag-Hwan (Sweet Persimmon Research Institute, Gyungsangnamdo Agricultural Research & Extension Services) ;
  • Park, Hae-Ryong (Department of Food Science and Biotechnology, Kyungnam University)
  • 발행 : 2011.08.30
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초록

This study was performed to assess the neuroprotective effects of methanolic extracts from sweet persimmon peel (PPE) against glutamate-induced cytotoxicity in hybridoma N18-RE-105 cells. The neuroprotective effects of PPE in N18-RE-105 cells were measured using the MTT reduction assay, LDH release assay, and phase-contrast microscopy. The results of the MTT reduction assay showed that treating cells with 500 ${\mu}g/ml$ PPE resulted in cell viability of 66.9%. Additionally, the morphological changes and the results of the LDH release assay showed that glutamate-induced damage to nerve cells was strongly inhibited by PPE. GSH content of N18-RE-105 cells was 3.5 ${\mu}M$ compared to that of the control, whereas pretreatment with 500 ${\mu}g/ml$ PPE increased GSH content by 4.7 ${\mu}M$. PPE was fractionated with hexane, and that layer had the highest neuroprotective effects in glutamate-stressed N18-RE-105 cells. In conclusion, our data showed that glutamate potentiated the effects of N18-RE-105 cell death by a mechanism involving oxidative stress. Therefore, PPE may be a potential candidate for prevention and therapy of neurodegenerative diseases.

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  2. Antioxidant activity of water extracts of persimmon flower buds vol.24, pp.1, 2015, https://doi.org/10.1007/s10068-015-0037-8
  3. Enhancement of Neuroprotective Effects of Spirulina maxima by a Low-temperature Extraction Process with Ultrasonic Pretreatment vol.23, pp.4, 2018, https://doi.org/10.1007/s12257-018-0066-2
  4. 청도반시(Diospyros kaki Thunb. cv. Cheongdo-Bansi) 탈삽 껍질 추출물의 산화스트레스로부터 PC-12 신경세포 보호 효과 vol.49, pp.5, 2017, https://doi.org/10.9721/kjfst.2017.49.5.538
  5. Deastringent Peel Extracts of Persimmon (Diospyros kaki Thunb. cv. Cheongdo-Bansi) Protect Neuronal PC-12 and SH-SY5Y Cells against Oxidative Stress vol.28, pp.7, 2011, https://doi.org/10.4014/jmb.1801.01013
  6. Enhancement of the Neuroprotective Effect of Fermented Spirulina maxima Associated with Antioxidant Activities by Ultrasonic Extraction vol.8, pp.12, 2011, https://doi.org/10.3390/app8122469