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중강도 운동훈련이 고지방식이 유도 비만쥐의 비장세포 염증성 사이토카인 발현에 미치는 영향

Effects of Moderate Exercise Training on Splenocyte Inflammatory Cytokine Production in High Fat Diet Induced Obese Mice

  • 장혁기 (서울여자대학교 체육학과) ;
  • 박희근 (충남대학교 스포츠과학과) ;
  • 정준현 (충남대학교 스포츠과학과) ;
  • 이왕록 (충남대학교 스포츠과학과)
  • Chang, Hyuk-Ki (Department of Human Movement Science, Seoul Women's University) ;
  • Park, Hee-Geun (Department of Sports Science, Chungnam National University) ;
  • Jeong, Jun-Hyun (Department of Sports Science, Chungnam National University) ;
  • Lee, Wang-Lok (Department of Sports Science, Chungnam National University)
  • 투고 : 2011.06.23
  • 심사 : 2011.08.04
  • 발행 : 2011.08.30

초록

본 연구는 고지방식이로 유도된 비만쥐를 대상으로 저지방식이와 중강도 운동이 비장세포의 전염증성 사이토카인 발현에 미치는 영향을 보고자 하였다. 4 주령의 C57BL/6 마우스를 대상으로 고지방식이(45% fat)를 5 주간 처치하여 비만을 유도한 후, 저지방식이(10% fat)로 전환한 후 통제군(운동미실시, n=10)과 운동군(중강도운동실시, n=10)으로 나누어 8 주간 트레드밀을 이용하여 중강도 운동을 주 5회, 1 일 30-60분 실시하였다. 운동종료후 비장세포를 분리하여 Concanavalin A (10 ug/ml)로 24시간 자극 후 전염증성 사이토카인인 IL-1${\beta}$, IL-6, TNF-${\alpha}$를 Bio-Plex를 활용하여 측정하였다. 자료분석은 독립 t 검증을 실시 하였으며, 유의수준은 p<0.05 수준으로 하였다. 비장세포에서 발현된 전염증성 사이토카인인 IL-1${\beta}$, IL-6, TNF-${\alpha}$는 저지방식이를 단독처치한 통제군에 비하여 중강도 운동을 병행실시한 운동군에서 유의하게 감소한 결과(p<0.01)를 나타내어 고지방식이로 유도된 비만쥐에서 저지방식이의 단독처치보다 중강도 운동의 병행 처치가 전염증 사이토카인 감소에 긍정적인 영향을 미치는 것으로 사료된다.

The present research was conducted to investigate the effect of moderate exercise training on splenocyte inflammatory cytokines production in high fat diet-induced obese mice. To determine the effects of exercise training and low fat diet on splenocyte, C57BL/6 male mice (4 weeks aged, n=20) were fed a high fat diet (45% fat diet) for 5 weeks so that obesity was achieved intentionally. These obese mice were then divided into 2 groups; HLC (low fat diet and control n=10), and HLE (low fat diet and exercise n=10). HLE mice performed 8 weeks of exercise training on a motorized treadmill by running for 30-60 min/day at 10-22 m/min, 0% grade, five times per week. After exercise training, all the splenocyte was collected and Con A (concanvalin A, 10${\mu}g/ml$) was used to stimulate the cells, after which IL-1${\beta}$, IL-6, TNF-${\alpha}$ were measured by bio plex. Independent t-test was used and p value under 0.05 was considered a statistical significance. Splenocyte IL-1${\beta}$, IL-6, TNF-${\alpha}$ production of HLE stimulated by Con A was significantly lower than that of HLC (p<0.01). These findings suggest that moderate exercise has beneficial effects on splenocyte inflammatory cytokines in high fat diet induced obese mice.

키워드

참고문헌

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피인용 문헌

  1. Effect of High-fat Diet on Peritoneal Macrophage Immunocompetence in C57/BL6 Mice vol.23, pp.6, 2013, https://doi.org/10.5352/JLS.2013.23.6.779
  2. Exercise training is more effective than resveratrol supplementation on alleviation of inflammation in peritoneal macrophages of high fat diet mice vol.18, pp.1, 2014, https://doi.org/10.5717/jenb.2014.18.1.79