DOI QR코드

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Anthocyanin Profiling and Radical Scavenging Activity of Selected Pigmented Rice Varieties

  • Ali, Hiba A. (Commission for Biotechnology and Genetic Engineering, National Center for Research) ;
  • Cho, Il-Kyu (Department of Molecular Biosciences and Bioengineering University of Hawaii at Manoa) ;
  • Kim, Sun-Ju (Department of Bio-Environment Chemistry, Chungnam National University) ;
  • Kim, Se-Na (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, So-Young (National Academy of Agricultural Science, Rural Development Administration) ;
  • Cho, Young-Sook (National Academy of Agricultural Science, Rural Development Administration) ;
  • Baek, Hyung-Jin (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Jung-Bong (National Academy of Agricultural Science, Rural Development Administration)
  • 투고 : 2011.06.02
  • 심사 : 2011.06.17
  • 발행 : 2011.06.30

초록

BACKGROUND: Anthocyanins have been recognized as health-enhancing substances due to their antioxidant activity, anti-inflammatory, anticancer, and hypoglycemic effects. The objective was to identify anthocyanins-rich rice grains for the development of functional foods and/or functional food colorants METHODS AND RESULTS: Rice grains of one black and three red-hulled rice varieties were extracted with acidified 80% aqueous methanol. The antioxidant activity of the methanolic extracts was screened on TLC plates and in an in vitro assay using DPPH (1, 1-diphenyl-2-picrylhydrazyl) as a free radical source. Red-hulled rice varieties exhibited higher antioxidant activity (88%, 1 mg/mL) than black rice (67%, 1 mg/mL). Among the red-hulled varieties tested, rice variety SSALBYEO54 (901452) was the most active (72%, 0.5 mg/mL). Rice extracted anthocyanin compounds were analyzed by HPLC-DAD-FLD and LC-MS/MS. Red-hulled varieties comprised cyanidin-3-glucoside in addition to ferulic acid esters, apigenin and kaempferol glycosides. CONCLUSION(s): Anthocyanins identified in the black rice variety were cyanidin-7-O-galactoside, cyanidin-3-Oglucoside, cyanidin-3'-O-glucoside, cyanidin-5-O-glucoside, cyanidin-3, 7-O-diglucoside, cyanidin-3, 5-O-diglucoside and peonidin-4'-O-glucoside. The results of this study show that the black rice (IT212512) and red-hulled rice variety SSALBYEO54 (901452) contain notable antioxidant activity for potential use in nutraceutical or functional food formulations.

키워드

참고문헌

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