Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Protective effects of a transgenic carrot vaccine on piglet diarrhea

자돈 설사병에 대한 형질전환 당근백신의 방어 효능 평가

  • Kim, Yeong-Hun (College of Veterinary Medicine and Institution of Veterinary Science, Kangwon National University) ;
  • Nam, Jin-Young (School of bioresource Sciences, Dankook University) ;
  • Lee, Hyang-Keun (School of bioresource Sciences, Dankook University) ;
  • Hwang, Cheol-Ho (School of bioresource Sciences, Dankook University) ;
  • Han, Jeong-Hee (College of Veterinary Medicine and Institution of Veterinary Science, Kangwon National University)
  • 김영훈 (강원대학교 수의과대학 및 동물의학종합연구소) ;
  • 남진영 (단국대학교 생명자원과학부) ;
  • 이향근 (단국대학교 생명자원과학부) ;
  • 황철호 (단국대학교 생명자원과학부) ;
  • 한정희 (강원대학교 수의과대학 및 동물의학종합연구소)
  • Accepted : 2011.03.14
  • Published : 2011.06.30
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Abstract

The study evaluated whether a transgenic carrot vaccine could induce a K88-specific immune response in sows and whether the resultant maternal antibody could protect piglets against enterotoxigenic Escherichia coli (ETEC) K88ac infection. Sows (n = 15) selected randomly from a farm in Korea were assigned to three groups (n = 5 per group: control [untreated]), group A (orally inoculated with a nontransgenic and transgenic carrot vaccines at 2 and 4 weeks ante partum, respectively), and group B (conventionally vaccinated according to the manufacturer's instructions). After 7 days of lactation, 5 piglets selected randomly from each group were challenged with $1{\times}10^{10}$ colony forming units/mL ETEC K88ac. Group C had the lowest mean fecal consistency score on post-challenge days 1 and 7. Histiologically, On post-challenge day 7, group C showed an increased duodenum and ileum villus:crypt ratio, compared to group A in the duodenum, with group B displaying the highest ratio. Groups B and C had more increased villus width than group A in the jejunum. Group C displayed the greatest increase in villus width in the ileum. The colostrums and serum from groups B and C displayed higher concentrations of IgA and IgG against ETEC K88, compared to group A. Based on the results, it was concluded that the transgenic carrot vaccine in sow per oral may have an effect on preventing piglet diarrhea as good as commercial recombinant vaccine.

Keywords

References

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