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우렁쉥이 추출물의 항산화 및 리파아제 저해활성 효과

Antioxidant and Anti-lipase Activity in Halocynthia roretzi Extracts

  • 권태형 (경북해양바이오산업연구원) ;
  • 김진기 (안동대학교 식품생명공학과) ;
  • 김태완 (안동대학교 식품생명공학과) ;
  • 이진욱 (경북해양바이오산업연구원) ;
  • 김준태 (경북해양바이오산업연구원) ;
  • 서현주 (안동대학교 식품생명공학과) ;
  • 김민정 (안동대학교 식품생명공학과) ;
  • 김충곤 (경북해양바이오산업연구원) ;
  • 전득산 (경북해양바이오산업연구원) ;
  • 박년호 (경북해양바이오산업연구원)
  • Kwon, Tae-Hyung (Gyeongbuk Institute for Marine Bio-Industry) ;
  • Kim, Jin-Ki (Food Science and Biotechnology Major, Andong National University) ;
  • Kim, Tae-Wan (Food Science and Biotechnology Major, Andong National University) ;
  • Lee, Jin-Wook (Gyeongbuk Institute for Marine Bio-Industry) ;
  • Kim, Jun-Tae (Gyeongbuk Institute for Marine Bio-Industry) ;
  • Seo, Hyun-Ju (Food Science and Biotechnology Major, Andong National University) ;
  • Kim, Min-Jeong (Food Science and Biotechnology Major, Andong National University) ;
  • Kim, Choong-Gon (Gyeongbuk Institute for Marine Bio-Industry) ;
  • Jeon, Deuk-San (Gyeongbuk Institute for Marine Bio-Industry) ;
  • Park, Nyun-Ho (Gyeongbuk Institute for Marine Bio-Industry)
  • 투고 : 2011.02.28
  • 심사 : 2011.04.22
  • 발행 : 2011.08.31

초록

본 연구에서는 동해안에 서식하는 우렁쉥이의 식품산업의 소재와 기능성을 알아보기 위하여 우렁쉥이 추출물을 이용하여 이들로부터 활성산소종의 소거활성, 즉 항산화제와 비만 예방의 효능제로서 생리활성을 살펴보고자 하였다. 우렁쉥이의 에탄올, 메탄올, 물 추출물을 이용하여 폴리페놀 및 플라보노이드 함량을 측정한 결과 에탄올 추출물과 메탄올 추출물이 물 추출물보다 약 1.5배 높게 나타났고 플라보노이드 함량 또한 약 8배 이상 높게 나타났다. 항산화 효능을 측정한 결과 DPPH 라디칼 소거능에서는 메탄올 추출물이 1176.99 ${\mu}g/mL$로 가장 높았으며 물 추출물에 비해 약 2배정도 높은 효능을 보였다. ABTS 라디칼 소거능 활성을 측정한 결과 875.07 ${\mu}g/mL$로 메탄올 추출물이 가장 높은 효능을 보였고 DPPH 라디칼 소거능 활성과 유사한 경향을 보였다. 환원력 측정은 메탄올 추출물 31.7%, 에탄올 추출물 32.2% 그리고 물 추출물 26.4%의 순으로 나타났으며 금속 봉쇄력의 경우는 물 추출물이 가장 높은 활성을 보였다. 폴리페놀 함량이 높은 물질이 항산화 효능과의 상관관계가 있음을 알 수 있었고 지방 분해 측정 결과 에탄올 추출물이 6004.76 ${\mu}g/mL$, 메탄올 추출물이 12021.56 ${\mu}g/mL$로 측정되었으며 에탄올 추출물에는 지방 분해 억제 효과가 있는 것으로 생각된다. 결과적으로 우렁쉥이는 항산화 효과와 비만예방 개선과 다양한 식품 소재로의 활용 가능성이 있을 것으로 사료되며, 이러한 결과가 실제로 체내에서 적용되는지는 in vivo 연구를 통하여 살펴볼 필요가 있는 것으로 사료된다.

Halocynthia roretzi is one of the most important cultured marine species on the southern coast of Korea. Samples were extracted using methanol (ME), ethanol (EE) and water (WE) to evaluate the antioxidant activities and antilipase activity in Halocynthia roretzi extracts. Antioxidant potentials of the samples were determined by poly-phenol content, flavonoid content, free radical scavenging activity, reducing potential, and chelating activity. The ME showed significant scavenging activity (1176 ${\mu}g/mL$ IC50 for DPPH, and 895 ${\mu}g/mL$ IC50 for ABTS assay). The IC50 for lipase inhibition activity was 12,021, 6,004, and 14,979 ${\mu}g/mL$ in the ME, EE, and WE, respectively. In conclusion, Halocynthia roretzi extracts exhibited antioxidant activities and anti-lipase activity. These results suggest that Halocynthia roretzi extracts can be potentially used as a source of antioxidant and antiobesity agents.

키워드

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