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분자주형을 이용한 요중 1-hydroxypyrene의 측정 방법 개발

Novel Method for Urinary 1-Hydroxypyrene Measurement Using Molecular Imprinting

  • 임동혁 (충북대학교 의과대학 예방의학교실 및 의학연구소) ;
  • 문선인 (충북대학교 의과대학 예방의학교실 및 의학연구소) ;
  • 최영숙 (충북대학교 의과대학 예방의학교실 및 의학연구소) ;
  • 박희진 (충북대학교 의과대학 예방의학교실 및 의학연구소) ;
  • 김대선 (국립환경과학원 환경건강위해성연구부 환경역학과) ;
  • 유승도 (국립환경과학원 환경건강위해성연구부 환경역학과) ;
  • 이철호 (한국환경공단 석면피해구제센터) ;
  • 김용대 (충북대학교 의과대학 예방의학교실 및 의학연구소) ;
  • 김헌 (충북대학교 의과대학 예방의학교실 및 의학연구소)
  • Yim, Dong-Hyuk (Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University) ;
  • Moon, Sun-In (Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University) ;
  • Choi, Young-Sook (Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University) ;
  • Park, Hee-Jin (Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University) ;
  • Kim, Dae-Seon (Environmental Health Research Division, Environmental Health Research Department, National Institute of Environmental Research) ;
  • Yu, Seung-Do (Environmental Health Research Division, Environmental Health Research Department, National Institute of Environmental Research) ;
  • Lee, Chul-Ho (Asbestos Damage Relief Center, Korea Environment Coporation) ;
  • Kim, Yong-Dae (Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University) ;
  • Kim, Heon (Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University)
  • 투고 : 2011.01.11
  • 심사 : 2011.03.09
  • 발행 : 2011.04.30

초록

본 연구는 선행연구에서 molecular imprinting 방법을 이용하여 개발한 1-OHP 인식 $TiO_2$-Bead가 실제 소변중의 1-OHP를 효율적으로 검출할 수 있는지 평가하기 위해서 시행되었다. 본 연구에서 개발한 분석 방법은 within-day 변이계수가 4.97%, between-day 변이계수가 4.43%로 나타나 본 측정방법이 매우 안정적인 방법임을 확인할 수 있었다. 1-OHP와 유사한 물질들이 혼합된 용액으로부터 $TiO_2$-Bead-HPLC 측정방법의 회수율을 평가한 결과, 평균 105.6%의 회수율을 나타내었다. 실제 소변시료를 대상으로 기존의 효소-HPLC 측정방법과 본 측정방법 간의 상관성을 평가한 결과, 두 방법 간에는 높은 상관관계(r=0.74, p<0.01)를 보여 bead를 이용한 요중 1-OHP의 새로운 측정 방법의 유용성을 확인할 수 있었다. 본 연구에서 개발한 새로운 측정방법은 기존의 측정방법에 비해 요중 1-OHP 농도를 보다 간편하고 저렴한 가격으로 측정할 수 있다는 장점이 있다. 본 연구의 결과는 $TiO_2$ 코팅 bead를 응용한 요중 1-OHP 센서의 개발에 응용할 수 있을 것으로 생각되며, 분자주형을 이용한 bead의 개발은 1-OHP 이외의 다른 화학물질의 분석에도 다양하게 응용할 수 있을 것으로 기대된다.

This study was performed to determine whether or not urinary 1-hydroxypyrene (1-OHP) levels can be accurately detected by our 1-OHP-detecting $TiO_2$-Bead-HPLC assay that we developed based on the molecular imprinting method. Our method showed a variation coefficient of 4.97% and a between-day variation coefficient of 4.43%, suggesting that this may be a very stable method. In addition, the recovery rate of 1-OHP from a mixture of 1-OHP and similar substances using our $TiO_2$-Bead-HPLC method was estimated to be 105.6%. The correlation coefficient between the conventional enzyme-HPLC method and this new method was 0.74 (p<0.01) when the urine samples were tested. Based on this result, it is conceivable that our method could be a useful technique for measuring urinary 1-OHP levels. Moreover, our method has some advantages of being easier and less expensive than the conventional method. The results of this study suggest that our method can facilitate the development of a urine 1-OHP sensor using $TiO_2$-coating beads and that development of beads by molecular imprinting can be applied to analysis of chemicals other than 1-OHP.

키워드

참고문헌

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