Effects of Acanthopanax Senticosus and Onion Mixture Extract on the Collagen-induced Arthritis in Rat Model

가시오가피와 양파 혼합 추출물이 Collagen 유발 관절염에 미치는 영향

  • 김경윤 (동신대학교 보건복지대학 물리치료학과) ;
  • 심기철 (동신대학교 보건복지대학 물리치료학과) ;
  • 김계엽 (동신대학교 보건복지대학 물리치료학과) ;
  • 최찬헌 (동신대학교 한의과대학) ;
  • 정재곤 (옥반식품 영농조합법인) ;
  • 정재선 (옥반식품 영농조합법인) ;
  • 정현우 (동신대학교 한의과대학)
  • Received : 2011.09.08
  • Accepted : 2011.12.08
  • Published : 2011.12.25

Abstract

This study was conducted to determine the analgesic effect of onion and acanthopanax senticosus mixture extract using the rheumatoid arthritis rat model. Rheumatoid arthritis model was made by the intradermal injection of type II collagen emulsified. Rats were divided into four groups: (1) Sham group(n=5), (2) Control group(administered DW 3 $m{\ell}$/1 day after RA induced, n=7), (3) Experimental group I(administered Onion extractor 600 mg/3 $m{\ell}$/1 day after RA induced, n=7). (4) Experimental group III(administered Onion and Acanthopanax senticosus mixture extractor 600 mg/3 $m{\ell}$/1 day after RA induced, n=7). After that, we examined the arthritic index, paw edema, pain threshold at 1st, 14th, 28th days. And also we examined histopathologic study(safranin-O green), immunohistochamical stain(COX-2) of knee joint at 28th days. Arthritic index, paw edema and pain threshold test were decrease in experimental group I, II than control group. Especially group II was most significantly inhibit effect than the other groups at 28th days. On the histopathologic view, all experimental groups were relieved and reproduced the erosion of arthritic site compared with control group. All experimental groups were COX-2 positive cells in the immunohistological stain of the knee joint were significantly decreased compared with control group. Especially group II was most significantly decreased than the other groups at 28th days. Onion and Acanthopanax senticosus mixture extractor can be used for curing rheumatoid arthritis. Anti-inflammatory effects may be somewhat better in combination of Onion and Acanthopanax senticosus.

Keywords

References

  1. 해리슨 번역 편찬위원회. 해리슨 내과학. 서울, 정담출판사, pp 2148-2157, 2006.
  2. 한방재활의학과학회. 한방재활의학과학. 서울, 군자출판사, pp 78-99, 2005.
  3. Song, Y.W. Updated pathophysiology of rheumatoid arthritis. Kor J Medicine, 76: 1-6, 2009.
  4. Park, S.H. New diagnostic method of rheumatoid arthritis. Kor J Medicine, 76: 7-11, 2009.
  5. Lorig, K., Holman, H. Arthritis self-management studies: A twelve years review. Health Educ Q, 20(1):17-28, 1993. https://doi.org/10.1177/109019819302000104
  6. Mun, M.S. A study on pain, discomfort, depression and coping patterns in chronic arthritis patients. J Rheumatol Health, 1(1):71-87, 1994.
  7. Kim, I.J., Suh, M.J. The related factors of self-efficacy in patients with Rheumatoid arthritis. J Korean Acad Adult Nurs, 11(1):87-95, 1999.
  8. 함상근. 류마티스 관절염의 약물치료. 가정의학회지 29: 320-323, 2008.
  9. 上海中醫學院編. 內科學(上). 上海, 上海科學技術出版社, pp 234-238, 1983.
  10. 鄭陽熙. 痺症의 分類와 治療에 관한 考察. 韓方物理療法學會誌 2: 56-66, 1991.
  11. 이윤숙, 박지용, 임혜자. 흰쥐의 급성 관절염 모델에서 NSAIDs의 말초 진통 효과와 Cyclooxygenase-1과 Cyclooxygenase-2의 발현. 대한마취과학회지 47: 862-869, 2004.
  12. Cashman, J.N. The mechanisms of action of NSAIDs in analgesia. Drugs, 52(5):13-23, 1996. https://doi.org/10.2165/00003495-199600525-00004
  13. Smith, W.L., DeWitt, D.L. Prostaglandin endoperoxide H synthases(cyclooxygenase)-1 and -2. Adv Immunol, 62: 167-215, 1996.
  14. Crofford, L.J., Wilder, R.L., Ristimaki, A.P., Sano, H., Remmers, E.F., Epps, H.R., Hla, T. Cyclooxygenase-1 and -2 expression in rheumatoid synovial tissues. J Clin Invest, 93: 1095-1101, 1194. https://doi.org/10.1172/JCI117060
  15. 이영호, 최성재, 지종대, 김애리, 김철환, 송관규. 류마티스 관절염 활액막에서 Cyclooxygensae-1, 2의 발현 양상. 대한류마티스학회지 5(2):159-168, 1998.
  16. You, J.H., Lee, K.K., Chan, T.Y., Lau, W.H., Han, F.K. Arthritis treatment in Hong Kong--cost analysis of celecoxib versus conventional NSAIDs, with or without gastroprotective agents. Aliment Pharmacol Ther, 16(12):2089-2096, 2002. https://doi.org/10.1046/j.1365-2036.2002.01376.x
  17. 윤형란, Michael Corzillius, 김성윤, 배상철. 류마티스 관절염 치료 시 비스테로이드성 소염제 단독, 위장관 부작용 예방 약제 병용 투여 및 COX-2 특이 억제제 사용의 한국 비용-효과 분석. 대한내과학회지 60(6);574-588, 2001.
  18. Feldman, M., Mcmahon, A.T. Do cyclooxygenase-2 inhibitors provide benefits similar to those of traditional nonsteroidal anti-inflammatory drugs, with less gastro-intestinal toxicity? Ann Intern Med, 132(2):134-143, 2000. https://doi.org/10.7326/0003-4819-132-2-200001180-00008
  19. Cannon, G.W. Cyclooxygenase-2 selective inhibitors. Drugs Today(Barc), 35(7):487-496, 1999.
  20. 신민교. 임상본초학, 서울, 영림사, pp 509-511, 1994.
  21. 전국 한의과대학 본초학교수. 본초학, 서울, 영림사, pp 283-284, 1994.
  22. 정보섭, 신민교. 鄕藥大事典, 서울, 영림사, pp 155-156, 1990.
  23. 임태수, 도정룡, 권오준, 김현구. 반응표면분석을 이용한 양파의 추출효율 및 기능성에 대한 모니터링. 한국식품영양과학회지 36(1):105-110, 2007.
  24. 정수진, 부산지역 중년 여성들의 건강기능식품에 대한 인지도 및 섭취실태, 부산대학교 대학원, 2009.
  25. 최찬헌, 김경윤, 정종길, 정재곤, 정현우. 양파와 가시오가피 혼합 추출액이 비만 흰쥐에 미치는 영향, 동의생리병리학회지 25(4):596-602, 2011.
  26. Trentham, D.E., Dynesius, R.A., Rockin, R.E., David, J.R. Cellular sensitivity to collagen in rheumatoid arthritis. New Engl J Med, 299: 327-332, 1978. https://doi.org/10.1056/NEJM197808172990703
  27. Winter, C.A., Risley, E.A., Nuss, G.W. Anti-inflammatory and antipyretic activities of indomethacin, 1-(P-chlorobenzoyl)-5-methoxy-2-methylindole-3-acetic acid. J Pharmacol Exp Ther, 141: 369-376, 1963.
  28. Lee, J.D. Korean Medicine approach to rheumatoid arthritis. J Kyung Hee Univ Med Cent, 20: 4-9, 2004.
  29. 김창수, 박용기. 우슬(牛膝)의 콜라겐 유도 관절염 생쥐에 대한 개선 효과. 大韓本草學會誌 25(4): 129-135, 2010.
  30. Simon, L.S., Yocum, D. New and future drug therapies for rheumatoid arthritis. Rheumatology (Oxford). 39(1):36-42, 2000. https://doi.org/10.1093/oxfordjournals.rheumatology.a031493
  31. 송정수. 류마티스 관절염 치료에서 종양괴사인자억제제 사용에 대한 고찰. 대한류마티스학회지 14(1):1-14, 2007.
  32. 송영욱. 최근 조명된 류마티스 관절염의 병태생리. 대한내과학회지 76(1):1-6, 2009.
  33. 최도영, 이재동, 백용현, 이송실, 유명철, 한정수, 양형인, 박상도, 유미현, 박은경, 박동석. 류마토이드 관절염에 대한 한약의 면역학적 연구동향. 대한침구학회지 21(4):177-196, 2004.
  34. Price, K.R., Bacon, J.R., Rhodes, M.J.C. Effect of storage and domestic processing on the content and composition of flavonol glycosides in Onion (Allium cepa). J Agric Food Chem, 45: 938-942, 1997. https://doi.org/10.1021/jf9605916
  35. Block, E., Gulati, H., Putman, D., You, N., Zhao, S.H. Allium chemistry: Synthesis of 1-[Alk(en)ylsulfinyl]propyl Alk(en)yl disulfides (cepaenes), antithrombotic flavorants from homogenates of Onion (Allium cepa). J Agric Food Chem, 45: 4414-4422, 1997. https://doi.org/10.1021/jf9705126
  36. Chu, Y.H., Chang, C.L., Hus, H.F. Flavonoid content of several vegetables and their antioxidant activity. J Sci Food Agric, 80: 561-566, 2000. https://doi.org/10.1002/(SICI)1097-0010(200004)80:5<561::AID-JSFA574>3.0.CO;2-#
  37. 박 원. 류마티스 관절염의 정의와 원인. 의약정보, 19(3): 29-33, 1993.
  38. 김진호, 한태륜. 재활의학. 서울, 군자출판사, p 503, 2003.
  39. Sluka, K.A., Westlund, K.N. Behavioral and immunohistochemical changes in an experimental arthritis model in rats. Pain, 55(3):367-377, 1993. https://doi.org/10.1016/0304-3959(93)90013-F
  40. Chen, S.H., Fahmi, H., Shi, Q., Benderdour M. Regulation of microsomal prostaglandin E2 synthase-1 and 5-lipoxygenase-activating protein/5-lipoxygenase by 4-hydroxynonenal in human osteoarthritic chondrocytes. Arthritis Res Ther, 12(1):R21, 2010. https://doi.org/10.1186/ar2926
  41. 이동훈, 손동섭, 조대윤, 김범준, 임윤영, 김영희. LPS로 활성화된 Raw 264.7 Cells에서 고삼 뿌리 추출물의 항염증과 항산화 효과. 대한의진균학회지 15(2):39-50, 2010.
  42. Kim, J.Y., Jung, K.S., Jeong, H.G. Suppressive effects of the kahweol and cafestol on cyclooxygenase-2 expression in macrophages. FEBS Lett, 569(1-3):321-326, 2004. https://doi.org/10.1016/j.febslet.2004.05.070
  43. Kim, R.G., Shin, K.M., Kim, Y.K., Jeong, H.J., Ha, J.H., Choi, J.W., Park, H.J., Lee, K.T. Inhibition of methanol extract from the aerial parts of Saururus chinensis on lipoplysaccharide-induced nitric oxide and prostaglandin E2 production from murine macrophage RAW 264.7 cells. Biol Pharm Bull, 26(4):481-486, 2003. https://doi.org/10.1248/bpb.26.481
  44. Issekutz, A.C., Issekutz, T.B. Quantification and kinetics of poly morphonuclear liukocyte and lymphocyte accumulation in joints during adjuvant arthritis in rats. Clin Immund Immuno Dathol, 61(3):436-447, 1991. https://doi.org/10.1016/S0090-1229(05)80014-5
  45. Saija, A., Scalese, M., Lanza, M., Marzullo, D., Bonina, F., Castelli, F. Flavonoids as antioxidant agents. Importance of their interaction with biomembranes. Free Radical Biol Med, 19: 481-486, 1995. https://doi.org/10.1016/0891-5849(94)00240-K
  46. 김호철. 한약약리학. 서울, 집문당. pp 204-205, 2001.
  47. 한방약리학 교재편찬위원회. 한방약리학. 서울, 도서출판 신일상사, pp 727-732, 2005.