Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Red Ginseng-Ejung-tang and White Ginseng-Ejung-tang Water Extract on Hydrogen Peroxide Production in RAW 264.7 Cells

백삼(白蔘)과 홍삼(紅蔘)이 포함된 이중탕(理中湯)의 마우스 대식세포 내 hydrogen peroxide 생성에 미치는 영향

  • Park, Wan-Su (Department of Pathology, College of Oriental Medicine, Kyungwon University)
  • 박완수 (경원대학교 한의과대학 병리학교실)
  • Received : 2010.10.22
  • Accepted : 2011.01.25
  • Published : 2011.02.25
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study is to investigate whether the intracellular hydrogen peroxide productions of mouse macrophage RAW 264.7 are modulated by Red Ginseng-Ejung-tang water extract (ER) and White Ginseng-Ejung-tang water extract (EG). Red Ginseng-Ejung-tang were composed of Red Ginseng, Atractylodes rhizome white, Zingiberis Rhizoma Siccus, and Glycyrrhizae Radix. White Ginseng-Ejung-tang were composed of White Ginseng, Atractylodes rhizome white, Zingiberis Rhizoma Siccus, and Glycyrrhizae Radix. The intracellular hydrogen peroxide productions were measured by dihydrorhodamine 123 assay with spectrofluorometer (excitation 485 nm; emission 535 nm). For 4, 20, 24, 44, 48, 68, and 72 h incubation, ER significantly increased hydrogen peroxide productions of RAW 264.7 at the concentration of 25, 50, 100, and $200{\mu}g/mL$ (P <0.05). EG for 4, 20, 24, 44, and 48 h incubation significantly increased hydrogen peroxide productions of RAW 264.7 at the concentration of 25, 50, 100, and $200{\mu}g/mL$ (P <0.05). For 68 and 72 h incubation, EG at the concentration of 50, 100, and $200{\mu}g/mL$ significantly increased hydrogen peroxide productions in RAW 264.7 (P <0.05). These results suggest that ER and EG have the immune-enhancing properties related with their increasing effects on the intracellular hydrogen peroxide production of macrophage.

Keywords

References

  1. 廣州中醫學院主編, 이상인, 김동찬, 이영종, 노승현, 주영승 공편역. 서울, 영림사, pp 144-146, 1990.
  2. 황도연. 대역증맥 방약합편. 서울, 남산당, pp 127-128, 1992.
  3. Guinazu, N., Carrera-Silva, E.A., Becerra, M.C., Pellegrini, A., Albesa, I., Gea, S. Induction of NADPH oxidase activity and reactive oxygen species production by a single Trypanosoma cruzi antigen. Int J Parasitol. 40(13):1531-1538, 2010. https://doi.org/10.1016/j.ijpara.2010.05.012
  4. Newman, S.L. Macrophages in host defense against Histoplasma capsulatum. Trends Microbiol. 7(2):67-71, 1999. https://doi.org/10.1016/S0966-842X(98)01431-0
  5. Supek, F., Supekova, L., Nelson, H., Nelson, N. Function of metal-ion homeostasis in the cell division cycle, mitochondrial protein processing, sensitivity to mycobacterial infection and brain function. J Exp Biol. 200(Pt 2):321-330, 1997.
  6. 박완수. EtOH 등의 독성물질에 대한 유산균발효애엽 추출물의 간세포보호효과. 동의생리병리학회지 24(3):457-462, 2010.
  7. 박완수. 마우스 대식세포(RAW 264.7)에 대한 艾葉물추출물의 생리활성 연구. 동의생리병리학회지 22(4):815-820, 2008.
  8. Jirapongsananuruk, O., Malech, H.L., Kuhns, D.B., Niemela, J.E., Brown, M.R., Anderson-Cohen, M., Fleisher, T.A. Diagnostic paradigm for evaluation of male patients with chronic granulomatous disease, based on the dihydrorhodamine 123 assay. J Allergy Clin Immunol. 111(2):374-379, 2003. https://doi.org/10.1067/mai.2003.58
  9. Richardson, M.P., Ayliffe, M.J., Helbert, M., Davies, E.G. A simple flow cytometry assay using dihydrorhodamine for the measurement of the neutrophil respiratory burst in whole blood: comparison with the quantitative nitrobluetetrazolium test. J Immunol Methods. 219(1-2):187-193, 1998. https://doi.org/10.1016/S0022-1759(98)00136-7
  10. Crow, J.P. Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species. Nitric Oxide. 1(2):145-157, 1997. https://doi.org/10.1006/niox.1996.0113
  11. van Pelt. L.J., van Zwieten, R., Weening, R.S., Roos, D., Verhoeven, A.J., Bolscher, B.G. Limitations on the use of dihydrorhodamine 123 for flow cytometric analysis of the neutrophil respiratory burst. J Immunol Methods. 191(2):187-196, 1996. https://doi.org/10.1016/0022-1759(96)00024-5
  12. Roesler, J., Hecht, M., Freihorst, J., Lohmann-Matthes, M.L., Emmendorffer, A. Diagnosis of chronic granulomatous disease and of its mode of inheritance by dihydrorhodamine 123 and flow microcytofluorometry. Eur J Pediatr. 150(3):161-165, 1991. https://doi.org/10.1007/BF01963557
  13. Bennett, M.K., Kirk, C.J. Development of proteasome inhibitors in oncology and autoimmune diseases. Curr Opin Drug Discov Devel. 11: 616-625, 2008.
  14. Ryan, J.G., Kastner, D.L. Fevers, genes, and innate immunity. Curr Top Microbiol Immunol. 321: 169-184, 2008.
  15. Gelderman, K.A., Hultqvist, M., Pizzolla, A., Zhao, M., Nandakumar, K.S., Mattsson, R., Holmdahl, R. Macrophages suppress T cell responses and arthritis development in mice by producing reactive oxygen species. J Clin Invest. 117(10):3020-3028, 2007. https://doi.org/10.1172/JCI31935
  16. Gelderman, K.A., Hultqvist, M., Olsson, L.M., Bauer, K., Pizzolla, A., Olofsson, P., Holmdahl, R. Rheumatoid arthritis: the role of reactive oxygen species in disease development and therapeutic strategies. Antioxid Redox Signal. 9(10):1541-1567, 2007. https://doi.org/10.1089/ars.2007.1569
  17. Hultqvist, M., Backlund, J., Bauer, K., Gelderman, K.A., Holmdahl, R. Lack of reactive oxygen species breaks T cell tolerance to collagen type II and allows development of arthritis in mice. J Immunol. 179(3):1431-1437, 2007.
  18. Hultqvist, M., Olofsson, P., Gelderman, K.A., Holmberg, J., Holmdahl, R. A new arthritis therapy with oxidative burst inducers. PLoS Med. 3(9):e348, 2006. https://doi.org/10.1371/journal.pmed.0030348
  19. Hultqvist, M., Holmdahl, R. Ncf1 (p47phox) polymorphism determines oxidative burst and the severity of arthritis in rats and mice. Cell Immunol. 233(2):97-101, 2005. https://doi.org/10.1016/j.cellimm.2005.04.008
  20. 하지용, 이 준. 加減理中湯의 BALB/c 마우스의 抗癌및 免疫調節作用에 미치는 影響加減理中湯의 BALB/c 마우스의 抗癌및 免疫調節作用에 미치는 影響. 동의병리학회지 12(2):73-81, 1998.