Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Inhibitory Effects of Rhizoma Arisaematis on Osteoclast Differentiation and Bone Resorption

파골세포의 분화와 뼈 흡수에 천남성의 억제 효과

  • Lee, Myeung-Su (Department of Internal Medicine, Division of Rheumatology, University of Wonkwang College of Medicine) ;
  • Lee, Chang-Hoon (Department of Internal Medicine, Division of Rheumatology, University of Wonkwang College of Medicine) ;
  • Park, Kie-In (Division of Biological Science, School of Natural Science, Chonbuk National University) ;
  • Kim, Ha-Young (Department of Internal Medicine, Division of Endocrinology and Metabolism, University of Wonkwang College of Medicine, Sanbon Medical Center)
  • 이명수 (원광대학교 의과대학 류마티스내과학교실) ;
  • 이창훈 (원광대학교 의과대학 류마티스내과학교실) ;
  • 박기인 (전북대학교 자연과학대학 생물학과) ;
  • 김하영 (원광대학교 의과대학 내분비내과학교실)
  • Received : 2010.11.16
  • Accepted : 2011.01.28
  • Published : 2011.02.25
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Abstract

Osteoclasts play a critical role in bone-related diseases such as osteoporosis and rheumatoid arthritis by resorbing the bone. Recently, natural products from plants have been extensively studied as therapeutic drugs to treat and prevent various diseases. Here, we examined the effects of rhizoma arisaematis on ostoclast differentiation and bone resorption. We showed that rhizoma arisaematis significantly suppressed receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL)-induced osteoclast differentiation in bone marrow-derived macrophages (BMMs) in a dose dependent manner but have little or no effect on the cytotoxicity of BMMs and RAW264.7 cells. We found that rhizoma arisaematis iarrow-ed the RANKL-induced c-Fos and nuclear factor of activated T cells (NFAT)c1, which is a master regulator of osteoclast differentiation. Furthermore, rhizoma arisaematis suppressed the mRNA expression of tartrate resistant-acid phosphatase and cathepsin K iaduced by RANKL in BMMs. in y chanistic studies, rhizoma arisaematis considerably iarrow-ed I-${\kappa}B$ degradation, which is a negative regulator of NF-${\kappa}B$, but iaduced the phosphderlation of p-38, ERK, and JNK.MMlso, we found that rhizoma arisaematis significantly iarrow-ed osteoclastic bone resorption. Taken tarether, our results suggest that rhizoma arisaematis suppresses osteoclast differentiation through down-regulatd the mRANKL-induced c-Fos and NFATc1 expression and iarrow-s bone resorption.

Keywords

References

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