Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Therapeutic Potential of Jeongjihwan for the Prevention and Treatment of Amnesia

정지환(定志丸)의 기억 및 인지기능 향상에 대한 효능 연구

  • Jung, Tae-Young (Department of Diagnostics, College of Oriental Medicine, Daeguhaany University) ;
  • Jeong, Won-Choon (Department of Diagnostics, College of Oriental Medicine, Daeguhaany University) ;
  • Park, Jong-Hyun (Department of Pathology, College of Oriental Medicine, Daeguhaany University)
  • 정태영 (대구한의대학교 한의과대학 진단학교실) ;
  • 정원춘 (대구한의대학교 한의과대학 진단학교실) ;
  • 박종현 (대구한의대학교 한의과대학 병리학교실)
  • Received : 2010.12.23
  • Accepted : 2011.01.25
  • Published : 2011.02.25
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Abstract

This study was aimed to investigate the memory enhancing effect of Jeongjihwan against scopolamine-induced amnesia in C57BL/6 mice. To determine the effect of Jeongjihwan on the memory and cognitive function, we have injected scopolamine (1 mg/kg, i.p.) into C57BL/6 mice 30 min before beginning of behavior tests. We have conducted Y-maze, Morris water-maze, passive avoidance and fear conditioning tests to compare learning and memory functions. Scopolamine-induced behavior changes of memory impairment were significantly restored by oral administration of Jeongjihwan (100 or 200 mg/kg/day). To elucidate the molecular mechanism underlying the memory enhancing effect of Jeongjihwan, we have examined the antioxidant defense system and neurotrophic factors. Jeongjihwan treatment attenuated intracellular accumulation of reactive oxygen species and up-regulated mRNA and protein expression of antioxidant enzymes as assessed by RT-PCR and western blot analysis, respectively. Jeongjihwan also increased protein levels of brain-derived neurotrophic factor (BDNF) compared with those in the scopolamine-treated group. Furthermore, as an upstream regulator, the activation of cAMP response element-binding protein (CREB) via phosphorylation was assessed by Western blot analysis. Jeongjihwan elevated the phosphorylation of CREB (p-CREB), which seemed to be mediated partly by extracellular signal-regulated kinase1/2 (ERK1/2) and protein kinase B/Akt. These findings suggest that Jeongjihwan may have preventive and therapeutic potential in the management of amnesia.

Keywords

References

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