The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Simultaneous determination of baicalin and glycyrrhizin in Sosiho-tang by HPLC and anti-inflammatory activity

소시호탕에서 baicalin과 glycyrrhizin 동시 분석 및 항염 활성

  • Kim, Jung-Hoon (Herbal Medicine EBM Research Center, Standard Research Division, Korea Institute of Oriental Medicine) ;
  • Ha, Hye-Kyung (Herbal Medicine EBM Research Center, Standard Research Division, Korea Institute of Oriental Medicine) ;
  • Seo, Chang-Seob (Herbal Medicine EBM Research Center, Standard Research Division, Korea Institute of Oriental Medicine) ;
  • Lee, Jin-Ah (Herbal Medicine EBM Research Center, Standard Research Division, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Herbal Medicine EBM Research Center, Standard Research Division, Korea Institute of Oriental Medicine)
  • 김정훈 (한국한의학연구원 표준화연구본부 한약EBM연구센터) ;
  • 하혜경 (한국한의학연구원 표준화연구본부 한약EBM연구센터) ;
  • 서창섭 (한국한의학연구원 표준화연구본부 한약EBM연구센터) ;
  • 이진아 (한국한의학연구원 표준화연구본부 한약EBM연구센터) ;
  • 신현규 (한국한의학연구원 표준화연구본부 한약EBM연구센터)
  • Received : 2011.02.03
  • Accepted : 2011.03.10
  • Published : 2011.03.30
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Abstract

Objectives : To quantitate the main compounds and investigate the biological activity of Sosiho-tang (Xiao-Chai-Hu-Tang, SST), simultaneous determination of baicalin and glycyrrhizin, and anti-inflammatory activity were estimated. Methods : A quantitative analysis was performed using a high performance liquid chromatography (HPLC). Reference compounds were separated on a reversed-phase column using gradient elution with water and acetonitrile each containing acetic acid at a flow rate of 1 mL/min. And the productions of nitric oxide (NO) and prostaglandin $(PE)E_2$ were examined by lipopolysaccharide (LPS)-treated RAW 264.7 cells in the presence of the SST. The anti-inflammatory activity of SST was investigated by carrageenin-induced paw edema in rats. The paw volume was measured at 2 and 4 hr following carrageenin-induced paw edema in rats. Results : The correlation coefficients of the compounds showed good linearity ($r^2$ > 0.9992) over the linear range. The precisions of intra- and inter-day were less than 7.0% of relative standard deviation (RSD) values for baicalin and less than 3.5% of RDS valuse for glycyrrhizin. Recovery rates were within the range of 95.41-101.5%. The contents of baicalin and glycyrrhizin in SST were average 70.52, 6.18 mg/g, respectively. And SST exhibited inhibitory effect on NO production in LPS-treated RAW 264.7 cells but not on $PGE_2$ production. Oral administration of SST (1 g/kg) showed a reduction in carrageenin-induced paw edema on rats. Conclusions : The analytical method was applied successfully to measure the contents of baicalin and glycyrrhizin in SST which exhibited anti-inflammatory activities.

Keywords

References

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