한국식품영양학회지 (The Korean Journal of Food And Nutrition)

  • 제24권2호
  • /
  • Pages.268-272
  • /
  • 2011
  • /
  • 1225-4339(pISSN)
  • /
  • 2287-4992(eISSN)
한국식품영양학회 (The Korean Society of Food and Nutrition)
권호 표지
DOI QR코드

DOI QR Code

대식세포에서 IRAK-4신호조절을 통한 기장(Panicum miliaceum L.)의 항염증능에 관한 연구

Anti-Inflammatory Activities of Hog Millet (Panicum miliaceum L.) in Murine Macrophages through IRAK-4 Signaling

  • 박미영 (국립농업과학원 농식품자원부 기능성식품과) ;
  • 김재현 (국립농업과학원 농식품자원부 기능성식품과) ;
  • 박동식 (국립농업과학원 농식품자원부 기능성식품과)
  • Park, Mi-Young (Functional Food & Nutrition Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Jae-Hyun (Functional Food & Nutrition Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Dong-Sik (Functional Food & Nutrition Division, National Academy of Agricultural Science, Rural Development Administration)
  • 투고 : 2011.03.14
  • 심사 : 2011.06.22
  • 발행 : 2011.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

전곡류의 섭취와 만성질환의 유병율은 음의 상관관계가 있는 것으로 알려져 있다. 본 연구에서는 선행 연구 결과, 지방축적억제능이 우수한 소재로 선정된 기장의 항염증능 여부를 검증하고자 하였다. 이를 위해 RAW264.7 세포에 기장열수분획($1\;{\mu}g/m\ell$ 및 $10\;{\mu}g/m\ell$)과 lipopolysaccharide(LPS)를 함께 처리한 후, 24시간 배양시켜 염증매개인자들의 분비량 및 mRNA 발현 정도를 측정하였다. 또한 LPS 자극에 대한 첫 번째 신호전달인자로 알려져 있는 interleukin-1 receptor associated kinase-4(IRAK-4)의 단백질 발현 정도를 측정하였다. 본 연구결과, 기장의 열수분획($10\;{\mu}g/m\ell$)은 LPS로 유도된 NO, $PGE_2$, TNF-${\alpha}$, IL-6 및 MCP-1의 생성량 및 mRNA 발현량을 유의적으로 억제하였다(p<0.05). 특히 이들 지표 중 pro-inflammatory cytokine인 TNF-${\alpha}$와 IL-6의 mRNA 발현량이 효과적으로 감소하였다(p<0.01). IRAK-4의 단백질 발현량 또한 유의적으로 감소하여 LPS 자극에 대한 기장열수분획의 항염증능은 toll-like receptor(TLR)를 통한 IRAK-4를 매개로 하는 신호전달체계 조절에 기인하는 것으로 사료된다.

키워드

참고문헌

  1. Akira S, Uematsu S, Takeuchi O. 2006. Pathogen recognition and innate immunity. Cell 124:783-801 https://doi.org/10.1016/j.cell.2006.02.015
  2. Asehnoune K, Strassheim D, Mitra S, Kim JY, Abraham E. 2004. Involvement of reactive oxygen species in Toll-like receptor 4-dependent activation of NF-kappa B. J Immunol 172:2522-2529 https://doi.org/10.4049/jimmunol.172.4.2522
  3. Bauer J, Ganter U, Geiger T, Jacobshagen U, Hirano T, Matsuda T, Kishimoto T, Andus T, Acs G, Gerok W, et al. 1988. Regulation of interleukin-6 expression in cultured human blood monocytes and monocyte-derived macrophages. Blood 72:1134-1140
  4. Carroll MB, Forgione MA. 2010. Use of tumor necrosis factor alpha inhibitors in hepatitis B surface antigen-positive patients: a literature review and potential mechanisms of action. Clin Rheumatol 29:1021-1029 https://doi.org/10.1007/s10067-010-1523-2
  5. Deshmane SL, Kremlev S, Amini S, Sawaya BE. 2009. Monocyte chemoattractant protein-1 (MCP-1): an overview. J Interferon Cytokine Res 29:313-326 https://doi.org/10.1089/jir.2008.0027
  6. Gabrovska D, Fiedlerova V, Holasova M, Maskova E, Smrcinov H, Rysova J, Winterova R, Michalova A, Hutar M. 2002. The nutritional evaluation of underutilized cereals and buckwheat. Food Nutr Bull 23:246-249
  7. Hirayama T, Tamaki Y, Takakubo Y, Iwazaki K, Sasaki K, Ogino T, Goodman SB, Konttinen YT, Takagi M. 2011. Toll-like receptors and their adaptors are regulated in macrophages after phagocytosis of lipopolysaccharide-coated titanium particles. J Orthop Res (in press)
  8. Hwang PA, Chien SY, Chan YL, Lu MK, Wu CH, Kong ZL, Wu CJ. 2011. Inhibition of lipopolysaccharide (LPS)-induced inflammatory responses by Sargassum hemiphyllum sulfated polysaccharide extract in RAW 264.7 macrophage cells. J Agric Food Chem 59:2062-2068 https://doi.org/10.1021/jf1043647
  9. Koziczak-Holbro M, Joyce C, Glück A, Kinzel B, Müller M, Gram H. 2007. Solving the IRAK-4 enigma: application of kinase-dead knock-in mice. Ernst Schering Found Symp Proc 3:63-82
  10. Kwak CS, Lim SJ, Kim SA, Park SC, Lee MS. 2004. Antioxidative and antimutagenic effects of Korean buckwheat, sorghum, millet and job's tears. J Korean Soc Food Sci Nutr 33:921-929 https://doi.org/10.3746/jkfn.2004.33.6.921
  11. Lee TW, Fedorak RN. 2010. Tumor necrosis factor-${\alpha}$ monoclonal antibodies in the treatment of inflammatory bowel disease: clinical practice pharmacology. Gastroenterol Clin North Am 39:543-557 https://doi.org/10.1016/j.gtc.2010.08.018
  12. Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) Method. Methods 25:402-408 https://doi.org/10.1006/meth.2001.1262
  13. Misra A, Rastogi K, Joshi SR. 2009. Whole grains and health: perspective for Asian Indians. J Assoc Physicians India 57: 155-162
  14. Ryu HS, Kim J, Kim HS. 2006. Enhancing effect of Sorghum bicolor L. Moench (Sorghum, su-su) extracts on mouse spleen and macrophage cell activation. Korean J Food & Nutr 19: 176-182
  15. Shenoi S, Wallace CA. 2010. Tumor necrosis factor inhibitors in the management of juvenile idiopathic arthritis: an evidencebased review. Paediatr Drugs 12:367-377 https://doi.org/10.2165/11532610-000000000-00000
  16. Song JE, Song JH, Cho SM, Min GH, Lee JS. 2010. Nutritional characteristics and physiological functionality of antidementia acetylcholinesterase inhibitor-containing methanol extract from Sorghum bicolor. Korean J Food & Nutr 23:226-232
  17. Takada E, Okahira S, Sasai M, Funami K, Seya T, Matsumoto M. 2007. C-terminal LRRs of human Toll-like receptor 3 control receptor dimerization and signal transmission. Mol Immunol 44:3633-3640 https://doi.org/10.1016/j.molimm.2007.04.021
  18. You X, Wu Y, Zeng Y, Deng Z, Qiu H, Yu M. 2008. Mycoplasma genitalium-derived lipid-associated membrane proteins induce activation of MAPKs, NF-kappaB and AP-1 in THP-1 cells. FEMS Immunol Med Microbiol 52:228-236 https://doi.org/10.1111/j.1574-695X.2007.00366.x

피인용 문헌

  1. Anticoagulant and Fibrinolytic Activities of Hwanggeumchal Sorghum In Vitro vol.23, pp.12, 2013, https://doi.org/10.5352/JLS.2013.23.12.1460
  2. Hog millet (Panicum miliaceumL.)-supplemented diet ameliorates hyperlipidemia and hepatic lipid accumulation in C57BL/6J-ob/obmice vol.5, pp.6, 2011, https://doi.org/10.4162/nrp.2011.5.6.511
  3. Pro-apoptotic and Anti-adipogenic Effects of Proso Millet (Panicum miliaceum) Grains on 3T3-L1 Preadipocytes vol.24, pp.5, 2014, https://doi.org/10.5352/JLS.2014.24.5.505
  4. Comparative Evaluation of Antioxidant Activities of Ethanol Extracts and Their Solvent Fractions Obtained from Selected Miscellaneous Cereal Grains vol.24, pp.1, 2014, https://doi.org/10.5352/JLS.2014.24.1.26
  5. Effects of natural raw meal (NRM) on high-fat diet and dextran sulfate sodium (DSS)-induced ulcerative colitis in C57BL/6J mice vol.9, pp.6, 2015, https://doi.org/10.4162/nrp.2015.9.6.619
  6. Erect Plant Type and Medium Maturing Proso millet (Panicum miliaceum L.) Variety 'Geumsilchal' vol.50, pp.3, 2018, https://doi.org/10.9787/kjbs.2018.50.3.296