Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Mixture of Bupleuri Radix and Lycii Radicis Cortex on the Change of HPA-Axis and Catecholamic System in the Forced Swimming Test

시호(柴胡)와 지골피(地骨皮) 합제(合劑)가 강제수영부하실험에서 HPA Axis와 카테콜라민 시스템에 미치는 영향

  • Cho, Eun-Ho (Pohang Seollin Oriental Medical Clinic Hospital) ;
  • Lee, Tae-Hee (Formulae Pharmacology Dept. Oriental Medical School, Kyung-Won University)
  • 조은호 (포항선린한방병원) ;
  • 이태희 (경원대학교 한의과대학 방제학교실)
  • Received : 2011.04.21
  • Accepted : 2011.06.13
  • Published : 2011.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : The effects of Extraction after Mixture of Bupleuri Radix and Lycii Radicis Cortex on the change of the HPA-Axis system and the Catecholamic system was investigated. Methods : After performing the Forced Swimming Test(FST), the expressions of corticotropin releasing factor(CRF), c-Fos in the paraventricular nucleus(PVN), and tyrosine hydroxylase(TH) in the ventral tegmental area(VTA) and locus coeruleus(LC) were measured by immunohistochemical method. Results : The duration of immobility in FST was significantly decreased in A 100(Extraction after Mixture of Bupleuri Radix and Lycii Radicis Cortex, 100mg/kg) and A 400(Extraction after Mixture of Bupleuri Radix and Lycii Radicis Cortex, 400mg/kg)(p<0.001). The expression of CRF in the PVN was shown the tendency to reduce in A100 and A400. The expression of c-Fos in the PVN was shown the tendency to reduce in A100 and A400. The expression of TH in the VTA was shown the tendency to reduce mildly in A100 and A400. The expression of TH in the LC was significantly reduced in A400(p<0.001). And the dose dependent reduction tendency was shown, respectively. Conclusions : According to the results above mentioned, the immobility, c-Fos and CRF expression was reduced at lower dose and was increased at higher dose. Therefore there is contradictory effects on the HPA Axis system in accordance with the dose. But in the effects on the catecholaminergic system, it significantly reduced the expression of TH in the LC. It was validated that the effect on the catecholaminergic system was ruled by Bupleuri Radix rather than Lycii Radicis Cortex via mainly the noradernergic system.

Keywords

References

  1. 대한신경정신의학회. 신경정신의학. 2판. 서울:중앙문화사. 2007:31, 169, 171.
  2. 백종우. 송지영. 대학생과 우울증. 경희의학. 2009;25(1):1-6.
  3. Mann JJ, Apter A, Bertolote J, Besutrais A, Currier D, Haas A. Suicide prevention strategies. Journal of the American Medical Association. 2005;294:2064-74. https://doi.org/10.1001/jama.294.16.2064
  4. Hagnell O, Lanke J, Rorsman B. Suicide rates in the Lundby study: mental illness as a risk factor for suicide. Neuropsychobiology. 1981;7:248-53. https://doi.org/10.1159/000117857
  5. 金相孝. 東醫神經精神科學. 서울:행림출판. 1996:211-2.
  6. 沈金鰲. 婦科玉尺. 北京:中醫古籍出版社. 1996:20-3.
  7. 張介賓, 景岳全書. 卷38. 서울:大星文化社. 1995:15.
  8. 武之望. 濟陰綱目. 서울:一中社. 1992:40-1.
  9. 조용국, 이태희. 柴胡抑肝湯이 스트레스로 인한 기억저하와 우울행동에 미치는 영향. 대한한의학 방제학회지. 2007;15(2):147-60.
  10. 박비순, 정진홍, 유동열. 시호억간탕의 항 stress 효능에 대한 연구. 대한한방부인과학회지. 2000;13(1):220-62.
  11. 김정훈, 이태희. 柴胡가 강제수영부하시험에서 CRF, c-Fos, ACTH와 TH에 미치는 효과. 대한본초학회지. 2009;24(2):29-37.
  12. 고동균, 이태희. 地骨皮가 스트레스로 인한 기억저하와 우울행동에 미치는 영향. 대한본초학회지. 2007;22(2):137-46.
  13. 구범모, 이태희. 地骨皮가 강제수영부하실험에서 HPA-Axis System에 미치는 영향. 대한한의학 방제학회지. 2010;18(2):215-25.
  14. 박수현, 이태희. 靑皮와 地骨皮 複合劑가 강제수영부하실험에서 CRF, c-Fos와 TH의 변화에 미치는 영향. 대한본초학회지. 2010;25(2):117-28.
  15. 신재용. 방약합편 해설. 서울:성보사. 1988:321.
  16. Porsolt RD, Bdrtin A, Jalfre M. Behavioural despair in rats: a new model sensitive to antidepressant treatments. European Journal Pharmacology. 1978;47(4):379-91. https://doi.org/10.1016/0014-2999(78)90118-8
  17. Arthur HS, Yueping Z, Adrian JD. Effects of chronic footshock, restraint and corticotropin-releasing factor on freezing, ultrasonic vocalization and forced swim behavior in rats. Behaviroal Brain Research. 2007;183:178-87. https://doi.org/10.1016/j.bbr.2007.06.006
  18. Paxinos G & Watson C. The Rat Brain In Stereotaxic Coordinates. 4th Edition. San Diego:Academic Press. 1998:26-7, 40-7, 56-62, 80-2, 95-100.
  19. 이정균. 정신의학. 서울:일조각. 1996:215-31.
  20. 金完熙, 崔達永. 臟腑辨證論治. 서울:成補社. 1993:141, 148, 149, 154, 155.
  21. 전국한의과대학 본초학교수 공편저. 本草學. 서울:영림사. 1994:149-50, 237-8.
  22. Gustavo ET, Renato B. Psychoneuroendocrinological links between chronic stress and depression. Progress in Neuro-Psychophannacology & Biological Psychiatiy 2003;27:893-903. https://doi.org/10.1016/S0278-5846(03)00162-3
  23. Muchimapura S, Fulford AJ, Mason R, Marsden CA. Isolation rearing in the rat disrupts the hippocampal response to stress. Neuroscience 2002;112:697-705. https://doi.org/10.1016/S0306-4522(02)00107-0
  24. Bullitt E. Expression of Fos-like protein as a marker for neuronal activity following noxious stimulation in the rat. The Journal of Comparative Neurology. 1990;296:517-30. https://doi.org/10.1002/cne.902960402
  25. Chrousos GP, Loriaux DL, Gold PW. Mechanisms of Physical and Emotional Stress. New York:Plenum. 1988:245.
  26. 대한신경정신의학회. 신경정신과학. 사울:하나의학사. 1998:72-6.
  27. Olschowka JA, O'Donohue TL, Mueller GP, Jacobowitz DM. Hypothalamic and extrahypothalamic distribution of CRF-like immunoreactive neurons in the rat brain. Neuroendocrinology. 1982;35:305-8. https://doi.org/10.1159/000123398
  28. Nemeroff CB, Widerlov E, Bissette G, Walleus H, Karlsson L, Eklund K, Kilts CD, Loosen PT. Elevated concentrations of CSF corticotropin-releasing factor-like immunoreactivity in depressed patients. Science. 1984;226:1342-4. https://doi.org/10.1126/science.6334362
  29. 방승규, 김우선. 절망행동모형에 의한 흰쥐 대뇌의 fos 발현의 증가. 신경정신의학. 1995;35(1):1949-55.
  30. 조성일, 이성필, 이창욱, 김우선, 백인호. Diazepam이 강박 스트레스 흰쥐 뇌의 Fos 발현에 미치는 영향. 신경정신의학. 1997;36(5):927-34.
  31. Melia KR, Ryabinin AE, Schroeder R, Bloom FE, Wilson MC. Induction and habituation of immediate early gene expression in rat brain by acute and repeated restraint stress. The Journal of Neuroscience. 1994;14:5929-38.
  32. Bowden C. Reduced dopamine turnover in the basal ganglia of depressed suicides. Brain Res. 1997;769(1):135-40. https://doi.org/10.1016/S0006-8993(97)00692-6
  33. Burn DJ. Depression and Parkinson's Disease. European Journal Neurology. 2002;9(3):44-54. https://doi.org/10.1046/j.1468-1331.9.s3.6.x
  34. 고경봉. 스트레스와 정신신체의학. 서울:(주)일조각. 2002:16-8.
  35. Beitner-Johnson D, Guitart X, Nestler EJ. Neurofilament proteins and the mesolimbic dopamine system : common regulation by chronic morphine and chronic cocaine in the rat ventral tegmental area. The Journal of Neuroscience. 1992;12(6):2165-76.
  36. Meng-Yang Z, Violetta K, Ginny ED, John WH, Craig S, James CO, Herbert YM, Gregory AO. Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biological Psychiatry. 1999;46(9):1275-86. https://doi.org/10.1016/S0006-3223(99)00135-3