한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)

  • 제44권3호
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  • Pages.225-231
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  • 2011
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  • 0374-8111(pISSN)
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  • 2287-8815(eISSN)
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
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해수순치에 따른 무지개송어 (Oncorhynchus mykiss)의 프로락틴 및 성장호르몬 유전자의 발현 변화

Changes in Prolactin and Growth Hormone Gene Expression of Rainbow Trout Oncorhynchus mykiss Adapted to Seawater

  • 신지혜 (강릉원주대학교 해양분자생명공학과) ;
  • 이철호 (수산자원사업단 양양연어사업소) ;
  • 조미희 (강릉원주대학교 해양분자생명공학과) ;
  • 홍관의 (수산자원사업단 양양연어사업소) ;
  • 김동수 (부경대학교 해양바이오신소재학과) ;
  • 손영창 (강릉원주대학교 해양분자생명공학과)
  • Shin, Ji-Hye (Department of Marine Molecular Biotechnology, Gangneung-Wonju National University) ;
  • Lee, Cheul-Ho (Yangyang Salmon Station, Korea Fisheries Resources Agency) ;
  • Jo, Mi-Hee (Department of Marine Molecular Biotechnology, Gangneung-Wonju National University) ;
  • Hong, Kwan-Eui (Yangyang Salmon Station, Korea Fisheries Resources Agency) ;
  • Kim, Dong-Soo (Department of Marine Bio-Materials and Aquaculture, Pukyung National University) ;
  • Sohn, Young-Chang (Department of Marine Molecular Biotechnology, Gangneung-Wonju National University)
  • 투고 : 2010.11.23
  • 심사 : 2011.06.10
  • 발행 : 2011.06.30
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초록

Prolactin (PRL) plays an important role in freshwater (FW) osmoregulation by preventing the loss of ions and the uptake of water in fish. Growth hormone (GH) promotes acclimation to seawater (SW) in several teleosts. We acclimated rainbow trout Oncorhynchus mykiss weighting $68.2{\pm}16.6$, $138.3{\pm}24$, and $287.5{\pm}42.1$ g in separate experiments to SW under slow-acclimation (SSW) or acute-acclimation (ASW) conditions, and then examined the PRL and GH mRNA levels using the real-time quantitative polymerase chain reaction. The PRL mRNA levels in all three experimental groups decreased significantly with both the SSW and ASW treatments, as compared to a control group kept in FW for 30 days. The GH mRNA levels increased with ASW in the largest fish, whereas the levels in the other groups did not change significantly. The mortality rate of the largest fish was lower than for the other groups, whereas the growth rate among the three experimental groups did not differ significantly. The growth rate of the ASW group was highest for the smallest fish. These results suggest that SW acclimation is associated with the gene expression levels of PRL and GH in relatively large rainbow trout. In addition, the fish mortality and growth rate on FW-SW transfer seem to be related to body weight, and the SW acclimation method may be applied to the hatcheries industry.

키워드

참고문헌

  1. Auperin B, Rentier-Delrue F, Martial JA and Prunet P. 1994. Evidence that two tilapia (Oreochromis niloticus) prolactins have different osmoregulatory functions during adaptation to a hyperosmotic environment. J Mol Endocrinol 12, 13-24. https://doi.org/10.1677/jme.0.0120013
  2. Auperin B, Leguen I, Rentier-Delrue F, Smal J and Prunet P. 1995. Absence of a tiGH effect on adaptability to brackish water in tilapia (Oreochromis niloticus). Gen Comp Endocrinol 97, 145-159. https://doi.org/10.1006/gcen.1995.1014
  3. Ayson FG, Kaneko T, Tagawa M, Hasegawa S, Grau EG, Nishioka RS, David SK, Bern HA and Hirano T. 1993. Effects of acclimation to hypertonic environment on plasma and pituitary levels of two prolactins and growth hormone in two species of tilapia, Oreochromis mossambicus and Oreochromis niloticus. Gen Comp Endocrinol 89, 138-148. https://doi.org/10.1006/gcen.1993.1017
  4. Baik KK, Choi YH, Lee JC, Park IS, Kim YK, Kim DY, Lee CS. 2007. Studies on seed production of rainbow trout, Oncorhynchus mykiss-hatching rate and early stage performance of USA strain rainbow trout, Oncorhynchus mykiss. J Aquaculture 20, 85-89.
  5. Bern HA and Madsen SS. 1992. A selective survey of the endocrine systerm of the rainbow trout (Oncorhynchus mykiss) with emphasis on the hormonal regulation of ion balance. Aquaculture 100, 237-262. https://doi.org/10.1016/0044-8486(92)90384-W
  6. Bjornsson BT, Stefansson SO and McCormick SD. 2010. Environmental endocrinology of salmon smoltification. Gen Comp Endocrinol in press.
  7. Boeuf G, Marc AM, Le Bail PY, Prunet P and Smal J. 1994. Stimulation of parr-smolt transformation by hormonal treatment in Atlantic salmon (Salmo salar L.). Aquaculture 121, 195-208. https://doi.org/10.1016/0044-8486(94)90020-5
  8. Chang YJ, Lee YC and Lee BK. 1996. Comparison of growth and survival rates of juvenile grey mullets (Mugil cephalus) in different salinities. J Aquaculture 9, 311-320.
  9. Chen JC, Lin JL. 1994. Osmolality and chloride concentration in the hemolymph of subadult Penaeus chinensis subjected to different salinity levels. Aquaculture 125, 167-174. https://doi.org/10.1016/0044-8486(94)90293-3
  10. Cho YM, Shin J and Sohn YC. 2006. Gene expression levels of growth hormone, prolactin and their receptors of olive flounder Paralichthys olivaceus by salinity changes. J Kor Fish Soc 39, 326-332. https://doi.org/10.5657/kfas.2006.39.4.326
  11. Choe MK and Yeo IK. 2002. Studies on the salinity tolerance of juvenile rainbow trout, Oncorhynchus mykiss. Korean J Ichthyol 14, 205-211.
  12. Dempson JB. 1993. Salinity tolerance of freshwater acclimated, small-sized arctic charr, Salvelinus alpinus from northern labrador. J Fish Biol 43, 451-462. https://doi.org/10.1111/j.1095-8649.1993.tb00580.x
  13. Donaldson EM, Fagerlund UHM, Higgs DA and McBride JR. 1979. Hormonal enhancement of growth in fish. In: Hoar, W.S., Randall, D.J., Brett, J.R. (Eds.), Fish Physiology: Bioenergetics and Growth, vol. 8. Academic Press, New York, U.S.A., 456-597.
  14. Farmer GJ and Beamish FWH. 1969. Oxygen consumption of Tilapia nilotica in relation to swimming speed and salinity. J Fish Res Bd Canada 26, 2807-2821. https://doi.org/10.1139/f69-277
  15. Fruchtman S, Jackson L and Borski R. 2000. Insulin-like growth factor I disparately regulates prolactin and growth hormone synthesis and secretion: studies using the teleost pituitary model. Endocrinology 141, 2886-2894. https://doi.org/10.1210/en.141.8.2886
  16. Heifetz J, Johnson SW, Koski KV and Murphy ML. 1989. Migration timing, size, and salinity tolerance of sea-type sockeye salmon (Oncorhynchus nerka) in an Alaska estuary. Can J Fish Aqua Sci 46, 633-637. https://doi.org/10.1139/f89-080
  17. Houston AH. 1961. Influence of size upon the adaptation of steelhead trout (Salmo gairdneri) and chum salmon (Oncorhynchus keta) to sea water. J Fish Res Board Can 18, 401-415. https://doi.org/10.1139/f61-036
  18. Jarvis PL and Ballantyne JS. 2003. Metabolic responses to salinity acclimation in juvenile shortnose sturgeon Acipenser brevirostrum. Aquaculture 219, 891-909. https://doi.org/10.1016/S0044-8486(03)00063-2
  19. Kaeriyama M, Shimizu I and Kakizaki H. 1987. Seasonal changes in seawater adaptation of sockeye salmon reared in the freshwater. Sci Rep Hokkaido Salm Hatch 41, 129-135.
  20. Kawauchi H and Sower SA. 2006. The dawn and evolution of hormones in the adenohypophysis. Gen Comp Endocrinol 148, 3-14. https://doi.org/10.1016/j.ygcen.2005.10.011
  21. Laiz-Carrion R, Fuentes J, Redruello B, Guzman JM, Martin del Rio MP, Power D and Mancera JM. 2009. Expression of pituitary prolactin, growth hormone and somatolactin is modified in response to different stressors (salinity, crowding and food-deprivation) in gilthead sea bream Sparus auratus. Gen Comp Endocrinol 162, 293-300. https://doi.org/10.1016/j.ygcen.2009.03.026
  22. Madsen SS and Bern HA. 1992. Antagonism of prolactin and growth hormone: impact on seawater adaptation in two salmonids, Salmo trutta and Oncorhynchus mykiss Zool Sci 9, 775-784.
  23. Maina JN. 1990. A study of the morphology of the gills of an extreme alkalinity and hyperosmotic adapted teleost Oreochromis alcalicus grahami (Boulenger) with particular emphasis on the ultrastructure of the chloride cells and their modifications with water dilution. A SEM and TEM study. Anat Embryol 181, 83-98.
  24. Mancera JM and McCormick SD. 1998. Evidence for growth hormone/insulin-like growth factor I axis regulation of seawater acclimation in the euryhaline teleost Fundulus heteroclitus. Gen Comp Endocrinol 111, 103-112. https://doi.org/10.1006/gcen.1998.7086
  25. Manzon LA. 2002. The role of prolactin in fish osmoregulation: a review. Gen Comp Endocrinol 125, 291-310. https://doi.org/10.1006/gcen.2001.7746
  26. McCormick SD, Sakamoto T, Hasegawa S and Hirano T. 1991. Osmoregulatory actions of insulin-like growth factor-I in rainbow trout (Oncorhynchus mykiss). J Endocrinol 130, 87-92. https://doi.org/10.1677/joe.0.1300087
  27. McLean E and Donaldson EM. 1993. The role of growth hormone in the growth of poikilotherms. In: The Endocrinology of Growth, Development, and Metabolism in Vertebrates. Schreibman MP, Scanes CG and Pang PKT, eds. Academic Press, New York, U.S.A., 43-71.
  28. Min BH, Choi CY and Chang YJ. 2005. Comparison of physiological conditions on black porgy, Acanthopagrus schlegeli acclimated and reared in freshwater and seawater. J Aquaculture 18, 37-44.
  29. Morgan JD and Iwama GK. 1991. Effects of salinity on growth, metabolism, and ion regulation in juvenile rainbow trout and steelhead trout (Oncorhynchus mykiss) and fall chinook salmon (Oncorhynchus tshawytscha). Can J Fish Aquat Sci 48, 2083- 2094. https://doi.org/10.1139/f91-247
  30. Park WD, Lee CH, Kim DJ and Sohn YC. 2008. Changes in prolactin and growth hormone gene expression in three freshwater teleosts with rapid changes in salinity. J Kor Fish Soc 41, 1-6.
  31. Parry G. 1958. Size and osmoregulation in fishes. Nature 181, 1218-1219. https://doi.org/10.1038/1811218a0
  32. Partridge GJ and Jenkins GJ. 2002. The effect of salinity on growth and survival of juvenile black bream (Acanthopagrus butcheri). Aquaculture 210, 219-230. https://doi.org/10.1016/S0044-8486(01)00817-1
  33. Pickering AD, Pottinger TG, Sumpter JP, Carragher JF and Le Bail PY. 1991. Effect of acute and chronic stress on the levels of circulating growth hormone in the rainbow trout, Oncorhynchus mykiss. Gen Comp Endocrinol 83, 86-93. https://doi.org/10.1016/0016-6480(91)90108-I
  34. Seale AP, Riley LG, Leedom TA, Kajimura S, Dores RM, Hirano T and Gordon Grau E. 2002. Effects of environmental osmolality on release of prolactin, growth hormone and ACTH from the tilapia pituitary. Gen Comp Endocrinol 128, 91-101. https://doi.org/10.1016/S0016-6480(02)00027-8
  35. Shepherd BS, Ron B, Burch A, Sparks R, Richman NHI, Shimoda SK, Stetson MH, Lim C and Grau EG. 1997. Effects of salinity, dietary level of protein and 17 alpha-methyltestosterone on growth hormone (GH) and prolactin (tPRL177 and tPRL188) levels in the tilapia, Oreochromis mossambicus. Fish Physiol Biochem 17, 279-288. https://doi.org/10.1023/A:1007770511491
  36. Shin J and Sohn YC. 2008. Molecular cloning of stanniocalcin 1 and its extracorpuscular regulation by salinity and $Ca^{2+}$ in the Japanese flounder. Zoolog Sci 25, 728-738. https://doi.org/10.2108/zsj.25.728
  37. Tomy S, Chang YM, Chen YH, Cao JC, Wang TP and Chang CF. 2009. Salinity effects on the expression of osmoregulatory genes in the euryhaline black porgy Acanthopagrus schlegeli. Gen Comp Endocrinol 161, 123-132. https://doi.org/10.1016/j.ygcen.2008.12.003
  38. Tsuzuki MY, Ogawa K, Strussmann CA, Maita M and Takashima F. 2001. Physiological responses during stress and subsequent recovery at different salinities in adult pejerrey Odontesthes bonariensis. Aquaculture 200, 349-362. https://doi.org/10.1016/S0044-8486(00)00573-1
  39. Wendelaar Bonga SE. 1997. The stress response in fish. Physiol Rev 77, 591-625.
  40. Yamaguchi K, Specker JL, King DS, Yokoo Y, Nishioka RS, Hirano T and Bern HA. 1988. Complete amino acid sequences of a pair of fish (tilapia) prolactins, tPRL177 and tPRL188. J Biol Chem 263, 9113-9121.