한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)

  • 제44권3호
  • /
  • Pages.216-224
  • /
  • 2011
  • /
  • 0374-8111(pISSN)
  • /
  • 2287-8815(eISSN)
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
권호 표지
DOI QR코드

DOI QR Code

매생이 (Capsosiphon fulvescens) 당단백질에 의한 인간 위암세포 사멸기전

Induction of Apoptosis Signaling by a Glycoprotein of Capsosiphon fulvescens in AGS Cell

  • Kim, Young-Min (Department of Food Science and Nutrition, Pukyong National University) ;
  • Kim, In-Hye (Department of Food Science and Nutrition, Pukyong National University) ;
  • Nam, Taek-Jeong (Department of Food Science and Nutrition, Pukyong National University)
  • 투고 : 2011.02.25
  • 심사 : 2011.05.30
  • 발행 : 2011.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Capsosiphon fulvescens is well-known green sea algae that, in recent years, has been proposed as a potential anticancer drug. In this study, we found that C. fulvescens glycoprotein (Cf-GP) had pro-apoptotic effects on human gastric carcinoma cells. By SDS-PAGE, we confirmed that C. fulvescens extract contained a glycoprotein. Using H33342 staining, we found that the Cf-GP caused cell death in a does-dependent manner, while an MTS assay showed decreased cellular viability due to induction of apoptosis. To determine the effect of Cf-GP on apoptosis-related cellular events, cells were treated with Cf-GP and the expression of several apoptosis-related protein was determined by Western blotting. Our results indicate that Cf-GP activated both a caspase cascade and PARP, which is a substrate of caspase-3, caspase-8 and the Bcl-2 family proteins. In addition, we assessed caspase-3, and -8 activation and annexin V staining. Our results revealed a cell cycle arrest, itself leading to an increased percentage of sub-G1 cells. Our findings indicate that Cf-GP may be a source of bio-functional material with therapeutic effects on human gastrointestinal cancer.

키워드

참고문헌

  1. Agarwal C, Singh RP and Agarwal R. 2002. Grape seed extract induces apoptotic death of human prostate carcinoma DU145 cells via caspases activation accompanied by dissipation of mitochondrial membrane potential and cytochrome c release. Carcinogenesis 23, 1869-1876. https://doi.org/10.1093/carcin/23.11.1869
  2. Alan GP and Reiner UJ. 1999. Emerging roles of caspase-3 in apoptosis. Cell Death and Differ 6, 99-104. https://doi.org/10.1038/sj.cdd.4400476
  3. Arends MJ, Morris RG, and Wylli AH. 1990. Apoptosis. The role of the endonucleasa. Am J Pathol 136, 593-608.
  4. Berg CP, Engels IH, Rothbart A, Lauber K, Renz A, Schlosser SF, Schulze-Osthoff K and Wesselberg S. 2001. Human mature red blood cells express caspase-3 and caspase-8, but are devoid of mitochondrial regulators of apoptosis. Cell Death Differ 8, 1197-1206. https://doi.org/10.1038/sj.cdd.4400905
  5. Bishop JM. 1987. The molecular genetics of cancer. Sci 235, 305-311. https://doi.org/10.1126/science.3541204
  6. Boo HJ, Hyun JH, Kim SC, Kang JI, Kim MK, Kim SY, Cho H, Yoo ES and Kim HK. 2011. Fucoidan from Undaria pinnatifida Induces Apoptosis in A549 Human Lung Carcinoma Cells. Phytother Res (online published)
  7. Elizabeth Y, Jiping Z, Jennifer J, Lawrence HB, Cralg BT and Korsmeyer SJ. 1995. Bad, a Heterodimeric Partner for Bcl-Xl and Bcl-2 Displaces Bax and Promotes Cell Death. Cell 80, 285-291. https://doi.org/10.1016/0092-8674(95)90411-5
  8. Emanuele S, D'Anneo A, Bellavia G, Vassallo B, Lauricella M, De Blasio A, Vento R and Tesoriere G. 2004. Sodium butyrate induces apoptosis in human hepatoma cells by a mitochondria/caspase pathway, associated with degradation of beta-catenin, pRb and Bcl-XL. Eur J Cancer 40, 1441-1452. https://doi.org/10.1016/j.ejca.2004.01.039
  9. Emily HY, Cheng A, Wei MC, Weiler S, Flavell RA, Mak TW, Lindsten T and Korsmeyer SJ. 2001. BCL-2, BCL-XL Sequester BH3 Domain-Only Molecules Preventing Bax- and BAK-Mediated Mitochondrial Apoptosis. Mol Cell 8, 705-711. https://doi.org/10.1016/S1097-2765(01)00320-3
  10. Funahashi H, Imai T, Mase T, Sekiya M, Yokoi K, Hayashi H, Shibata A, Hayashi T, Nishikawa M, Suda N, Hibi Y, Mizuno Y, Tsukamura K, Hayakawa A and Tanuma S. 2001. Seaweed Prevents Breast Cancer? Japan J Cancer Res 92, 483-487. https://doi.org/10.1111/j.1349-7006.2001.tb01119.x
  11. Gerlier D and Thomasset N. 1986. Use of MTT colorimetric assay to measure cell activation. J Immunol Methods. 94, 57-63. https://doi.org/10.1016/0022-1759(86)90215-2
  12. Go H, Hwang HJ, and Nam TJ. 2010. A glycoprotein from Lamimnaria japonica induces apoptosis in HT-29 colon cancer cells. Toxicol in Vitro 24, 1546-1553. https://doi.org/10.1016/j.tiv.2010.06.018
  13. Jang KJ, Han MH, Lee BH, Kim BW, Kim CH, Yoon HM and Choi YH. 2010. Induction of Apoptosis by Ethanal Extracts of Ganoderma lucidum in Human Gastric Carcinoma Cells. J Acupunct Meridian Stud 3, 24-31. https://doi.org/10.1016/S2005-2901(10)60004-0
  14. John FRK, Clay MW and Brian VH. 1994 Apoptosis. Its significance in cancer and cancer Theraphy. Cancer 73, 2013-2026. https://doi.org/10.1002/1097-0142(19940415)73:8<2013::AID-CNCR2820730802>3.0.CO;2-J
  15. Jung KJ, Jung CH, Pyeun JH and Choi YJ. 2005. Changes of Food Components in Mesangi (Capsosiphon fulvecense), Gashiparae (Enteromorpha prolifera), and Cheonggak (Codium fragile) Depending on Harvest Times. J Korean Soc Food Sci Nutr 34, 687-693. https://doi.org/10.3746/jkfn.2005.34.5.687
  16. Kim EJ, Lee YJ, Shin HK and Park JHY. 2006. A Study on the Mechanisms by Which the Aqueous Extract of Inonotus obliquus Induces Apoptosis and Inhibits Proliferation in HT-29 Human Colon Cancer Cells. J Korean Soc Food Sci Nutr 35, 516-523. https://doi.org/10.3746/jkfn.2006.35.5.516
  17. Kim HS. 2009. Helicobacter Pylori Infection and Gastric Cancer. Helicobactor and Gastrointestinal Research 9, 22-25.
  18. Kim IH, Lee HH, Jang JS, Lee SH, Ha JM, Ha JB, and Lee JH. 2006. Antioxidant and Antimicrobial Activities of Seaweed, Ulva lactuca. Environmental Mutagens & Carcinogens 26, 48-52.
  19. Kwon MJ and Nam TJ. 2006. Porphyran induces apoptosis related signal pathway in AGS gastric cancer cell lines. Life Sci 79, 1956-1962. https://doi.org/10.1016/j.lfs.2006.06.031
  20. Laemmli UK. 1970. Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227, 680-685. https://doi.org/10.1038/227680a0
  21. Lavrik I, Golks A and Krammer PH. 2005. Death receptor signaling. J Cell Sci 118, 265-267. https://doi.org/10.1242/jcs.01610
  22. Lee KH, Kwon HJ, Chun SS, Kim JH, Cho YJ, and Cha WS. 2006. Biological Activities of Extracts from Phellinus linteus. J Korean Soc Appl Biol Chem 49, 298-303.
  23. Hwang HJ, Kim IH and Nam TJ. 2008. Food and Chemical Effect of a glycoprotein from Hizikia fusiformis on acetaminophen-induced liver injury. Food and Chemic Toxicol 46, 3475-3481. https://doi.org/10.1016/j.fct.2008.08.032
  24. Honglin Li, Zhu H, Xu Cj, and Yuan J. 1998. Cleavage of BID by Caspase 8 Mediates the Mitochondrial Damage in the Fas Pathway of Apoptosis. Cell 94, 491-501. https://doi.org/10.1016/S0092-8674(00)81590-1
  25. Nagata S. 1997. Apoptosis by death factor. Cell 88, 355-365. https://doi.org/10.1016/S0092-8674(00)81874-7
  26. Noda H, Amano H, Arashima K and Nisizawa K. 1990. Antitumor activity of marine algae. Hydrobiologia 204/205, 577-584.
  27. Odile C and Felix RA. 2009. Role of PARP-1 and PARP-2 in the expression of apoptosis-regulating gene in HeLa cells. Cell Biol Toxicol 25, 379-391. https://doi.org/10.1007/s10565-008-9092-8
  28. Park HS, Lee YS, Koo SJ, Han MJ, Choue RW and Oh SY. 2003. Diet and health 2nd ed Hyoil, Seoul, Korea
  29. Park HY, Lim CW, Kim YK, Yoon HD and Lee KJ. 2006. Immunostimulating and Anticancer Activities of Hot Water Extract from Capsosiphon fulvescens. J Korean Soc Appl Biol Chem 49, 343-348.
  30. Park YC, Jeong JH, Park KJ, Choi HJ, Park YM, Jeong BK, Higuchi Y and Yoo YH. 2005. Sulindac activates nuclear translocation of AIF, DFF40 and endonuclease G but not induces oligonucleosomal DNA fragmentation in HT-29 cells. Life Sci 77, 2059-2070. https://doi.org/10.1016/j.lfs.2005.04.021
  31. Shen HM, Dai J, Chia SE, Lim A and Ong CN. 2002. Detection of apoptotic alterations in sperm in subfertile patients and their correlations with sperm quality. Hum Reprod 17, 1266-1273. https://doi.org/10.1093/humrep/17.5.1266
  32. Sudheer KM, Som DS, and Santosh KK. 2006. Berbrine, a natural product, induces G1-phase cell cycle arrest and caspase-3-dependent apoptosis in human prostate carcinoma cells. Mol Cancer Ther 5, 296-308. https://doi.org/10.1158/1535-7163.MCT-05-0448
  33. Takahashi A, Alnemri ES, Lazebnik YA, Fernandes- Alnemri T Litwack G, Moir RD, Goldman RD, Poirier GG, Kaufmann SH and Earnshaw WC. 1996. Cleavage of lamin A by Mch2 alpha but not CPP32: multiple interleukin 1 beta- converting enzyme-related proteases with distinct substrate recognition properties are active in apoptosis. Proc Natl Acas Sci USA 93, 8395-8400. https://doi.org/10.1073/pnas.93.16.8395
  34. Yutaka E, Shigeomi S and Yoshihide T. 1997. Intracellular ATP Levels Determine Cell Death Fate by Apoptosis. Cancer Research 57, 1835-1840.