Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Prevalence of Clostridium difficile Infections in Pigs in Jeju

제주도 돼지에서 Clostridium difficile 감염 양상

  • Kim, Hyeong-Seok (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Jung, Ji-Youl (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Kang, Sang-Chul (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Shin, Bo-Moon (Department of Laboratory Medicine, Sanggye-Paik Hospital, Inje University) ;
  • Lee, Eun-Joo (Department of Laboratory Medicine, Sanggye-Paik Hospital, Inje University) ;
  • Hwang, Eui-Kyung (College of Life Science and Natural Resources, Sangji University) ;
  • Park, Bong-Kyun (College of Veterinary Medicine, Seoul National University) ;
  • Kim, Dae-Yong (College of Veterinary Medicine, Seoul National University) ;
  • Kim, Jae-Hoon (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
  • 김형석 (제주대학교 수의과대학, 수의과학연구소) ;
  • 정지열 (제주대학교 수의과대학, 수의과학연구소) ;
  • 강상철 (제주대학교 수의과대학, 수의과학연구소) ;
  • 신보문 (인제의대 상계백병원 진단검사의학과) ;
  • 이은주 (인제의대 상계백병원 진단검사의학과) ;
  • 황의경 (상지대학교 생명자원과학대학) ;
  • 박봉균 (서울대학교 수의과대학) ;
  • 김대용 (서울대학교 수의과대학) ;
  • 김재훈 (제주대학교 수의과대학, 수의과학연구소)
  • Accepted : 2011.04.04
  • Published : 2011.04.30
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Abstract

Clostridium (C) difficile has been recognized as an important emerging pathogen in both humans and animals. The prevalence of C. difficile in rectal feces and frozen colons of 132 pigs with diarrhea from the Jeju Island was investigated by polymerase chain reaction (PCR) to detect C. difficile toxin A and B genes. PCR findings revealed toxin A and B in 5 pigs (3.8%), including 2 suckling pigs, 2 weaned pigs and 1 growing pig. The result of PCR was closely matched histopathologic lesions of C. difficile in large intestines of pigs. Histopathologically, the cecum and colons of C. difficile toxin-positive pigs had severe submucosal and mesocolonic edema. Mucosal lesions ranged from random single cell necrosis and exfoliation to segmental, transmural necrosis of the cecum and colon. According to bacteriology, 4 C. difficile-positive pigs (80%) were co-infected with Salmonella typhimurium.

Clostridium (C.) difficile 은 사람뿐만 아니라 동물에서도 새롭게 출현한 중요한 병원체로 인식되어 왔다. 제주도에서 설사 증상이 있는 132두의 돼지 직장 분변 및 냉동 결장을 대상으로 C. difficile 균체의 독소 A, B 유전자를 검출하기 위한 중합효소연쇄반응을 실시하였다. 그 결과 포유자돈 2두 이유자돈 2두 및 육성단계 돼지 1두 등 총 5두의 돼지에서 독소 A 및 B에 대하여 양성반응을 나타내었다. PCR 결과는 돼지 대장의 조직학적 병변과 일치하였다. 병리조직학적 소견으로 독소 양성 돼지의 맹장과 결장에서는 점막하직 및 장막의 부종이 관찰되었다. 점막의 병변은 국소적인 상피세포의 괴사, 부분적인 탈락 및 장벽의 괴사까지 다양하였다. 세균 검사 결과 4두 (80%)는 Salmonella typhimurium과 혼합감염되어 있었다.

Keywords

References

  1. Alvarez-Perez S, Blanco JL, Bouza E, Alba P, Gibert X, Maldonado J, Garcia ME. Prevalence of Clostridium difficile in diarrhoeic and non-darrhoeic piglets. Vet Microbiol 2009; 137: 302-305. https://doi.org/10.1016/j.vetmic.2009.01.015
  2. Gould LH, Limbago B. Clostridium difficile in food and domestic animals: a new foodborne pathogen? Clin Infect Dis 2010; 51: 577-582. https://doi.org/10.1086/655692
  3. Jones MA, Hunter D. Isolation of Clostridium difficile from pigs. Vet Rec 1983; 112: 253. https://doi.org/10.1136/vr.112.11.253
  4. Jung K, Ha SK, Chung HK, Kim J, Cho WS, Choi C, Chae C. Archival PCR-based diagnosis of Clostridium difficile in piglets. Vet Rec 2003; 153: 466-467 https://doi.org/10.1136/vr.153.15.466
  5. Keel MK, Songer JG. The comparative pathology of Clostridium difficile-associated disease. Vet Pathol 2006; 43: 225-240 https://doi.org/10.1354/vp.43-3-225
  6. Kiss D, Bilkei G. A new periparturient disease in eastern Europe, Clostridium difficile causes postparturient sow losses. Theriogenology 2005; 63: 17-23 https://doi.org/10.1016/j.theriogenology.2004.02.020
  7. Lyerly DM, Krivan HC, Wilkins TD. Clostridium difficile: its disease and toxins. Clin Microbiol Rev 1988; 1: 1-18
  8. Shin BM, Lee EJ. Comparison of toxin A enzyme linked fluorescence assay and latex agglutination based on Clostridium difficile culture and toxin A and B PCR assay. Korean J Clin Microbiol 2005; 8: 130-135.
  9. Shin BM, Kuak EY. Chracterization of a toxin A-negative, toxin B-positive variant strain of Clostridium difficile. Korean J Lab Med 2006; 26: 27-31 https://doi.org/10.3343/kjlm.2006.26.1.27
  10. Songer JG. Clostridia as agents of zoonotic disease. Vet Microbiol 2010; 140: 399-404. https://doi.org/10.1016/j.vetmic.2009.07.003
  11. Songer JG, Uzal FA. Clostridial enteric infections in pigs. J Vet Diagn Invest 2005; 17: 528-536. https://doi.org/10.1177/104063870501700602
  12. Waters EH, Orr JP, Clark EG, Schaufele CM. Typhlocolitis caused by Clostridium difficile in sucking piglets. J Vet Diagn Invest 1998; 10: 104-108 https://doi.org/10.1177/104063879801000122
  13. Yaeger M, Funk N, Hoffman L. A survey of agents associated with neonatal diarrhea in Iowa swine including Clostridium difficile and porcine reproductive and respiratory syndrome virus. J Vet Diagn Invest 2002; 14: 281-287. https://doi.org/10.1177/104063870201400402
  14. Yaeger MJ, Kinyon JM, Songer JG. A retrospective, case control study evaluating the association between Clostridium difficile toxins in the colon of neonatal swine and gross and microscopic lesions. J Vet Diagn Invest 2007; 19: 52-59. https://doi.org/10.1177/104063870701900108