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The Korean Society of Plant Biotechnology (한국식물생명공학회)
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In vitro propagation of Bambusa nutans Wall. ex Munro through axillary shoot proliferation

  • Received : 2010.05.19
  • Accepted : 2010.10.29
  • Published : 2011.01.31
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Abstract

This communication describes for the first time an efficient and reproducible protocol for large-scale multiplication of Bambusa nutans. Nodal segments collected from field-grown clumps and cultured on Murashige and Skoog (MS) medium supplemented with $4.4{\mu}M$ benzylaminopurine (BA) and $2.32{\mu}M$ kinetin (Kin) gelled with 0.2% gelrite yielded 80% aseptic cultures with 100% bud-break. The in vitro-formed shoots obtained after bud-break were successfully multiplied in MS liquid medium supplemented with $13.2{\mu}M$ BA, $2.32{\mu}M$ Kin, and $0.98{\mu}M$ indole-3-butyric acid (IBA). Sub-culturing of shoots every 3 weeks on fresh multiplication medium yielded a consistent proliferation rate of 3.5-fold. Shoot clusters containing three to five shoots were successfully rooted with 100% success on half-strength MS liquid medium supplemented with $9.8{\mu}M$ IBA, $2.85{\mu}M$ indole-3-acetic acid (IAA), $2.68{\mu}M$ naphthaleneacetic acid (NAA), and 3% sucrose. Plantlets grown in vitro were acclimatized and subsequently transferred to the field. Inter-simple sequence repeat analysis has confirmed the genetic uniformity of the tissue-cultured plants up to 27 passages.

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References

  1. Arya S, Rana PK, Sharma R, Arya ID (2006) Tissue culture technology for rapid multiplication of Dendrocalamus giganteus Munro. Indian For 132:345-357
  2. Bornet B, Branchard M (2001) Nonanchored Inter Simple Sequence Repeat (ISSR) markers: reproducible and specific tools for genome fingerprinting. Plant Mol Biol Rep 19:209-215 https://doi.org/10.1007/BF02772892
  3. Chowdhury P, Das M, Sikdar SR, Pal A (2004) Influence of the physiological age and position of the nodal explants on micropropagation of field-grown Dendrocalamus strictus Nees. Plant Cell Biotech Mol Biol 5:45-50
  4. Das M, Pal A (2005) In vitro regeneration of Bambusa balcooa Roxb.: factors affecting changes of morphogenetic competence in the axillary buds. Plant Cell Tissue Organ Cult 81:109-112 https://doi.org/10.1007/s11240-004-3017-x
  5. Debergh P (1983) Effects of agar brand and concentration on the tissue culture medium. Physiol Plant 59:270-276 https://doi.org/10.1111/j.1399-3054.1983.tb00770.x
  6. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13-15
  7. Driver JA, Suttle GR (1987) Nursery handling of propagules. In: Bonga JM, Durzan DJ (eds) Cell and tissue culture in forestry. Kluwer, Dordrecht, pp 320-335
  8. Jimenez VM, Castillo J, Tavares E, Guevara E, Montiel M (2006) In vitro propagation of the neotropical giant bamboo, Guadua angustifolia Kunth, through axillary shoot proliferation. Plant Cell Tissue Organ Cult 86:389-395 https://doi.org/10.1007/s11240-006-9120-4
  9. Kalia S, Kalia RK, Sharma SK (2004) In vitro regeneration of an indigenous bamboo (Bambusa nutans) from internode and leaf explant. J Bamboo Rattan 3:217-228 https://doi.org/10.1163/1569159041765272
  10. Kapoor P, Rao IU (2006) In vitro rhizome induction and plantlet formation from multiple shoots in Bambusa bambos var gigantea Bennet and Gaur by using growth regulators and sucrose. Plant Cell Tissue Organ Cult 85:211-217 https://doi.org/10.1007/s11240-005-9074-y
  11. Mishra Y, Patel PK, Yadav S, Shirin F, Ansari SA (2008) A micropropagation system for cloning of Bambusa tulda Roxb. Sci Hortic 115(3):315-318 https://doi.org/10.1016/j.scienta.2007.10.002
  12. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-479 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  13. Nagaoke T, Ogihara Y (1997) Applicability of Inter-Simple Sequence Repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers. Theor Appl Genet 94:597-602 https://doi.org/10.1007/s001220050456
  14. Negi D, Saxena S (2009) Ascertaining clonal fidelity of tissue culture raised plants of Bambusa balcooa Roxb. using inter simple sequence repeat markers. New For 40(1):1-8. doi:10.1007/s11056-009-9182-3
  15. Ramanayake SMSD, Wanniarachchi WAVR, Tennakoon TMA (2001) Axillary shoot proliferation and in vitro flowering in an adult giant bamboo, Dendrocalamus giganteus Wall ex. Munro. In Vitro Cell Dev Biol Plant 37:667-671 https://doi.org/10.1007/s11627-001-0116-9
  16. Sanjaya, Rathore TS, Ravishankar Rai V (2005) Micropropagation of Pseudoxytenanthera stocksii Munro. In Vitro Cell Dev Biol Plant 41:333-337 https://doi.org/10.1079/IVP2004625
  17. Saxena S (1990) In vitro propagation of the bamboo (Bambusa tulda Roxb.) through shoot proliferation. Plant Cell Rep 9:431-434
  18. Saxena S, Bhojwani SS (1993) In vitro clonal multiplication of 4-year old plants of the bamboo, Dendrocalamus longispathus Kurz. In Vitro Cell Dev Biol Plant 29:135-142
  19. Shenoy VB, Vasil IK (1992) Biochemical and molecular analysis of plants derived from embryogenic tissue cultures of napier grass (Pannisetum purpureum K Schum). Theor Appl Genet 83:947-955
  20. Singh S, Ansari SA, Kumar P (2002) Clonal propagation of Bambusa nutans through culm and culm-branch cuttings. Indian For 128:35-40
  21. Stapleton C (1998) Form and function in the bamboo rhizome. J Am Bamboo Soc 12:21-29
  22. Tewari DN (1992) A monograph on bamboo. International Book Distributors, Dehra Dun
  23. Tornero OP, Egea J, Olmos E, Burgos L (2001) Control of hyperhydricity in micropropagated apricot cultivars. In Vitro Cell Dev Biol Plant 37:250-254 https://doi.org/10.1007/s11627-001-0044-8
  24. Tsumara Y, Ohba K, Strauss SH (1996) Diversity and inheritance of inter-simple sequence repeat polymorphisms in Douglas-fir (Pseudotsuga menziesii) and sugi (Cryptomeria japonica). Theor Appl Genet 92:40-45 https://doi.org/10.1007/BF00222949
  25. Yasodha R, Sumathi R, Malliga P, Gurumurthi K (1997) Genetic enhancement and mass production of quality propagules of Bambusa nutans and Dendrocalamus membranaceous. Indian For 123:303-306
  26. Yasodha R, Kamala S, Anand Kumar SP, Durai Kumar P, Kalaiarasi K (2008) Effect of glucose on in vitro rooting of mature plants of Bambusa nutans. Sci Hortic 116(1):113-116 https://doi.org/10.1016/j.scienta.2007.10.025
  27. Zimmerman TW, Rogers SMD, Cobb BG (1991) Controlling vitrification of Petunia in vitro. In Vitro Cell Dev Biol Plant 27:165-167 https://doi.org/10.1007/BF02632211
  28. Ziv M (1991) Quality of micropropagated plants—vitrification. In Vitro Cell Dev Biol Plant 27:64-69 https://doi.org/10.1007/BF02632130

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