Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Overexpression of jasmonic acid carboxyl methyltransferase increases tuber yield and size in transgenic potato

  • Sohn, Hwang-Bae (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University) ;
  • Lee, Han-Yong (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University) ;
  • Seo, Ju-Seok (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University) ;
  • Jung, Choon-Kyun (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University) ;
  • Jeon, Jae-Heung (Plant Cell Biotechnology Lab, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Jeong-Han (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University) ;
  • Lee, Yin-Won (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University) ;
  • Lee, Jong-Seob (School of Biological Sciences, Seoul National University) ;
  • Cheong, Jong-Joo (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University) ;
  • Choi, Yang-Do (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University)
  • Received : 2010.09.03
  • Accepted : 2010.10.15
  • Published : 2011.01.31
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Abstract

Jasmonates control diverse plant developmental processes, such as seed germination, flower, fruit and seed development, senescence and tuberization in potato. To understand the role of methyl jasmonate (MeJA) in potato tuberization, the Arabidopsis JMT gene encoding jasmonic acid carboxyl methyltransferase was constitutively overexpressed in transgenic potato plants. Increases in tuber yield and size as well as in vitro tuberization frequency were observed in transgenic plants. These were correlated with JMT mRNA level-- the higher expression level, the higher the tuber yield and size. The levels of jasmonic acid (JA), MeJA and tuberonic acid (TA) were also higher than those in control plants. Transgenic plants also exhibited higher expression of jasmonate-responsive genes such as those for allene oxide cyclase (AOC) and proteinase inhibitor II (PINII). These results indicate that JMT overexpression induces jasmonate biosynthesis genes and thus JA and TA pools in transgenic potatoes. This results in enhanced tuber yield and size in transgenic potato plants.

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References

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