Anti-metastatic Effects of Celastrus orbiculatus Extract in B16F10 Melanoma Cells

  • Jeon, Hoon (College of Pharmacy, Woosuk University)
  • Received : 2011.02.28
  • Accepted : 2011.05.28
  • Published : 2011.06.30

Abstract

Celastrus orbiculatus has been widely used as a traditional medicine for the treatment of many diseases including rheumatoid arthritis and odontalgia. In the present study, anti-metastatic activity of a methanolic extract from C. orbiculatus (MCO) was studied. A gelatin zymographic assay revealed that MCO has potent inhibitory effects on MMP-2 and MMP-9 activities in B16F10 melanoma cells. Moreover, MCO attenuated MMP expression via down-regulation of NF-${\kappa}$B translocation to the nucleus. Melanoma cell migration and invasion were also down-regulated by MCO. In addition, MCO significantly suppressed lung metastasis in an in vivo model. These results strongly suggest that MCO may possibly be used as a valuable anti-metastatic agent for cancer treatment.

Keywords

References

  1. Arvelo, F. and Cotte, C., Metalloproteinases in tumor progression. Review Invest. Clin. 47, 185-205 (2006).
  2. Baek, W.K., Park, J.W., Lim, J.H., Suh, S.I., Suh, M.H., Gabrielson, E., and Kwon, T.K., Molecular cloning and characterization of the human budding uninhibited by benomyl (BUB3) promoter. Gene 295, 117- 123 (2002). https://doi.org/10.1016/S0378-1119(02)00827-2
  3. Gao, Z., Sasaoka, T., Fujimori, T., Oya, T., Ishii, Y., Sabit, H., Kawaguchi, M., Kurotaki, Y., Naito, M., Wada, T., Ishizawa, S., Kobayashi, M., Nabeshima, Y., and Sasahara, M., Deletion of the PDGFR-beta gene affects key fibroblast functions important for wound healing. J. Biol. Chem. 280, 9375-9389 (2005). https://doi.org/10.1074/jbc.M413081200
  4. Guo, Y.Q., Li, X., Li, J.J., Xu, J., Li, N., Meng, D.L., and Wang, J.H., A new sesquiterpene ester inhibiting no production from the fruits of Celastrus orbiculatus. J. Asian Nat. Prod. Res. 8, 739-742 (2006). https://doi.org/10.1080/10286020412331286498
  5. Hrabec, E., Strek, M., Nowak, D., Greger, J., Suwalski, M., and Hrabec, Z., Activity of type IV collagenases (MMP-2 and MMP-9) in primary pulmonary carcinomas: a quantitative analysis. J. Cancer Res. Clin. Oncol. 128, 197-204 (2002). https://doi.org/10.1007/s00432-001-0320-3
  6. Hwang B.Y., Kim, H.S., Lee, J.H., Hong, Y.S., Ro, J.S., Lee, K.S., and Lee, J.J., Antioxidant benzoylated flavan-3-ol glycoside from Celastrus orbiculatus. J. Nat. Prod. 64, 82-84 (2001). https://doi.org/10.1021/np000251l
  7. Jiang, J., Grieb, B., Thyagarajan, A., and Sliva, D., Ganoderic acids suppress growth and invasive behavior of breast cancer cells by modulating AP-1 and NF-$\kappa$B signaling. Int. J. Mol. Med. 21, 577-584 (2008).
  8. Johnsen, M., Lund, L.R., Romer, J., Almholt, K., and Dano, K., Cancer invasion and tissue remodeling: common themes in proteolytic matrix degradation. Curr. Opin. Cell Biol. 10, 667-671 (1998). https://doi.org/10.1016/S0955-0674(98)80044-6
  9. Kohn, E.C. and Liotta, L.A., Molecular insights into cancer invasion: strategies for prevention and intervention. Cancer Res. 55, 1856-1862 (1995).
  10. Lee, J.H., Koo, T.H., Yoon, H., Jung, H.S., Jin, H.Z., Lee, K., Hong, Y.S., and Lee, J.J., Inhibition of NF-kappa B activation through targeting I kappa B kinase by celastrol, a quinone methide triterpenoid. Biochem. Pharmacol. 72, 1311-1321 (2006). https://doi.org/10.1016/j.bcp.2006.08.014
  11. Liabakk, N.B., Talbot, I., Smith, R.A., Wilkinson, K., and Balkwill, F., Matrix metalloprotease 2 (MMP-2) and matrix metalloprotease 9 (MMP-9) type IV collagenases in colorectal cancer. Cancer Res. 56, 190-196 (1996).
  12. Lind, D.S., Hochwald, S.N., Malaty, J., Rekkas, S., Heby, P., and Mishra, G., Nuclear factor-B is unregulated in colorectal cancer. Surgery 130, 363-369 (2001). https://doi.org/10.1067/msy.2001.116672
  13. McCawley, L.J. and Matrisian, L.M., Matrix metalloproteinases: multifunctional contributors to tumor progression. Mol. Med. Today 6, 149-156 (2000). https://doi.org/10.1016/S1357-4310(00)01686-5
  14. Min, K.R., Hwang, B.Y., Lim, H.S., Kang, B.S., Oh, G.J., Lee, J., Kang, S.H., Lee, K.S., Ro, J.S., and Kim, Y., (−)-Epiafzelechin: cyclooxygenase-1 inhibitor and anti-inflammatory agent from aerial parts of Celastrus orbiculatus. Planta Medica 65, 460-462 (1999). https://doi.org/10.1055/s-2006-960813
  15. Nakshatri, H., Bhat-Nakshatri, P., Martin, D.A., Goulet, R.J., and Sledge, G.W., Constitutive activation of $NF-{\kappa}B$ during progression of breast cancer to hormone independent growth. Mol. Cell Biol. 17, 3629-3639 (1997). https://doi.org/10.1128/MCB.17.7.3629
  16. Oppenheimer, S.B., Cellular basis of cancer metastasis: A review of fundamentals and new advances. Acta Histochem. 108, 327-334 (2006). https://doi.org/10.1016/j.acthis.2006.03.008
  17. Scorilas, A., Karameris, A., Arnogiannaki, N., Ardavanis, A., Bassilopoulos, P., Trangas, T., and Talieri, M., Overexpression of matrix-metalloproteinase-9 in human breast cancer: a potential favourable indicator in node-negative patients. Br. J. Cancer 84, 1488- 1496 (2001). https://doi.org/10.1054/bjoc.2001.1810
  18. Tryggvason, K., Hoyhtya, M., and Salo, T., Proteolytic degradation of extracellularmatrix in tumor invasion. Biochim. Biophys. 907, 191-217 (1987).
  19. Weiss, L., Metastatic inefficiency. Adv. Cancer Res. 54, 159-211 (1990).
  20. Yadav, V.R., Sung, B., Prasad, S., Kannappan, R., Cho, S.G., Liu, M., Chaturvedi, M.M., and Aggarwal, B.B., Celastrol suppresses invasion of colon and pancreatic cancer cells through the downregulation of expression of CXCR4 chemokine receptor. J. Mol. Med. 88, 1243- 1253 (2010). https://doi.org/10.1007/s00109-010-0669-3
  21. Yang, J. and Richmond, A., Constitutive IkappaB kinase activity correlates with nuclear factor-kappaB activation in human melanoma cells. Cancer Res. 61, 4901-4909 (2001).
  22. Yoon, S.O., Park, S.J., Yoon, S.Y., Yun, C.H., and Chung, A.S., Sustained production of H2O2 activates pro-matrixmetalloproteinase-2 through receptor tyrosine kinases/ phosphatidylinositol 3-kinase/NF-kappa B pathway. J. Biol. Chem. 277, 30271-30282 (2002). https://doi.org/10.1074/jbc.M202647200
  23. Yoon, S.O., Park, S.J., Yun, C.H., and Chung, A.S., Roles of matrix metalloproteinases in tumor metastasis and angiogenesis. J. Biochem. Mol. Biol. 36, 128-137 (2003). https://doi.org/10.5483/BMBRep.2003.36.1.128
  24. Zhang, J., Xu, Y.M., Wang, W.M., and Liu, Y.Q., Experimental study on antitumor effect of extract from Celastrus orbiculatus in vivo. China J. Chinese Materia Medica 31, 1514-1516 (2006).
  25. Zhang, L.J., Zhu, R.Q., Fei, Y., Feng, G., Lei, D.L., and Zhao, X.L., Experimental study on inhibiting angiogenesis in tumor by celastrol. Cancer Res. Prevent. Treat. 32, 719-720 (2005).
  26. Zhu, H., Liu, X.W., Cai, T.Y., Cao, J., Tu, C.X., Lu, W., He, Q.J., and Yang, B., Celastrol acts as a potent antimetastatic agent targeting beta1 integrin and inhibiting cell-extracellular matrix adhesion, in part via the p38 mitogen-activated protein kinase pathway. J. Pharmacol. Exp. Ther. 334, 489-499 (2010). https://doi.org/10.1124/jpet.110.165654