Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Surveillance of Newcastle Disease Virus in Chicken Slaughterhouses

닭 도축장에서의 뉴캣슬병 바이러스 오염 실태 조사

  • Choi, Kang-Seuk (Avian Disease Division, National Veterinary Research and Quarantine Service) ;
  • Lee, Eun-Kyoung (Avian Disease Division, National Veterinary Research and Quarantine Service) ;
  • Jeon, Woo-Jin (Avian Disease Division, National Veterinary Research and Quarantine Service) ;
  • Kwon, Jun-Hun (Avian Disease Division, National Veterinary Research and Quarantine Service) ;
  • Lee, Jin-Hwa (College of Veterinary Medicine, Kangwon National University) ;
  • Sung, Haan-Woo (College of Veterinary Medicine, Kangwon National University)
  • 최강석 (국립수의과학검역원 조류질병과) ;
  • 이은경 (국립수의과학검역원 조류질병과) ;
  • 전우진 (국립수의과학검역원 조류질병과) ;
  • 권준헌 (국립수의과학검역원 조류질병과) ;
  • 이진화 (강원대학교 수의과대학) ;
  • 성환우 (강원대학교 수의과대학)
  • Received : 2011.03.02
  • Accepted : 2011.06.07
  • Published : 2011.06.30
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Abstract

We conducted a 10-month (March to October 2009) surveillance of Newcastle disease virus (NDV) in 13 slaughterhouses in Korea. NDV was isolated in 13.0%, 13.3%, 16.0%, and 10.8% of chicken farms, transport vehicles, hang rooms, and chilling water, respectively. Of NDV isolates from slaughterhouses, 37% were isolated in July. All NDV isolates were determined to be lentogenic viruses by RT-PCR-based pathotyping, and all NDV isolates had the $^{112}GKQGR/L^{117}$ motif at the cleavage site of the F protein. Phylogenetic analysis based on the hypervariable region of the F protein gene classified all 25 NDV isolates examined into genotype I within class II. Of these, 24 were clustered together with the NDV V4 strain, while the remaining isolate was placed in the cluster belonging to the NDV Ulster 2C strain. Our results indicate that lentogenic NDV was a high-frequency contaminant in the serial process of ranging live birds to slaughtering at slaughterhouses.

국내 도축장 및 도축장 출하 닭을 대상으로 NDV 오염 실태를 조사하였다. 조사결과, 닭출하농장, 닭수송용차량, 도축장 현수실, 도축장 냉각수에서 각각 조사 대상건수의 13.0%, 13.3%, 16.0%, 10.8%에서 NDV가 분리되었다. 시기별로 보면 차량, 현수실, 냉각수 모두에서 7월에 가장 많이 바이러스가 분리되었다. Pathotyping RT-PCR을 실시한 결과, 이들 분리된 NDV 분리주는 모두 저병원성 NDV 양성 반응을 나타내었으며, F 단백질 분절 부위에 모두 $^{112}GKQGR/L^{117}$ motif를 가지고 있었다. 본 연구에서 분리된 NDV 분리주의 F 단백질 유전자를 분석하여 보았을 때, 조사한 NDV 분리주 25주 중 24주가 NDV V4주와 같은 유전적 cluster를 형성하였으며, 나머지 1주는 NDV Ulster 2C주와 같은 유전적 cluster를 형성하였다. 이와 같은 연구 결과로 보아 국내에서는 출하 닭, 수송 차량, 현수실, 냉각수 등 생산에서 도축 단계까지 일련의 과정에서 높은 빈도로 저병원성 NDV 오염이 이루어지고 있음을 알 수 있었다.

Keywords

References

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