Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Determination of hesperidin in mixed tea by HPLC

HPLC를 이용한 혼합차의 Hesperidin 정량

  • Han, Saem (Department of Integrative Plant Science, Chung-Ang University) ;
  • Mok, So-Youn (Department of Integrative Plant Science, Chung-Ang University) ;
  • Kim, Hye-Min (Department of Integrative Plant Science, Chung-Ang University) ;
  • Lee, Jeong-Min (Department of Integrative Plant Science, Chung-Ang University) ;
  • Lee, Dong-Gu (Department of Integrative Plant Science, Chung-Ang University) ;
  • Lee, Sook-Young (Department of Food and Nutrition, Chung-Ang University) ;
  • Kim, Jong-Kee (Department of Integrative Plant Science, Chung-Ang University) ;
  • Kim, Sun-Ju (Department of Bio-Environmental Chemistry, Chungnam National University) ;
  • Lee, Sang-Hyun (Department of Integrative Plant Science, Chung-Ang University)
  • 한샘 (중앙대학교 식물시스템과학과) ;
  • 목소연 (중앙대학교 식물시스템과학과) ;
  • 김혜민 (중앙대학교 식물시스템과학과) ;
  • 이정민 (중앙대학교 식물시스템과학과) ;
  • 이동구 (중앙대학교 식물시스템과학과) ;
  • 이숙영 (중앙대학교 식품영양학과) ;
  • 김종기 (중앙대학교 식물시스템과학과) ;
  • 김선주 (충남대학교 생물환경화학과) ;
  • 이상현 (중앙대학교 식물시스템과학과)
  • Received : 2011.05.03
  • Accepted : 2011.06.20
  • Published : 2011.06.30
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Abstract

The content of hesperidin in the mixed tea, which was composed of dried orange peel, laurel leaf, mulberry leaf, silver magnolia leaf, oriental melon tap, cassia seed, and licorice root, was determined by high performance liquid chromatography (HPLC). Hesperidin was quantified by a reverse phase column with gradient solvent system (watcr:acetonitrile = 80:20 to 35:65 for 30 min) and UV/VIS detection (280 nm). The How rate was kept constant at 1.0 ml/min. The content of hesperidin in the mixed tea was measured in depending on extraction time 1, 2, 3, and 4 min (29.07, 52.39, 52.45, and 88.35 mg/g, respectively).

Keywords

References

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