Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Identification and quantification of glucosinolates in rocket salad (Eruca sativa)

Rocket salad(Eruca sativa) 중 glucosinolate 동정 및 정량

  • Kim, Cho-Rok (Department of Bio-Environmental Chemistry, Chungnam National University) ;
  • Lim, Young-Sil (Department of Bio-Environmental Chemistry, Chungnam National University) ;
  • Lee, Sang-Won (Herbal Crop Research Team, International Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Sun-Ju (Department of Bio-Environmental Chemistry, Chungnam National University)
  • 김초록 (충남대학교 생물환경화학과) ;
  • 임영실 (충남대학교 생물환경화학과) ;
  • 이상원 (농촌진흥청 국립원예특작과학원 인삼특작부 인삼약초가공팀) ;
  • 김선주 (충남대학교 생물환경화학과)
  • Received : 2011.03.14
  • Accepted : 2011.06.20
  • Published : 2011.06.30
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Abstract

Glucosinolate (GSL) contents were investigated (i) at 1~7 days after sowing (DAS) in seed sprouts and (ii) at 3-7 weeks after sowing for the time-course. Moreover, (iii) They were compared with five different cultivars of rocket salad (Eruca sativa). Seventeen GSLs were separated by HPLC analysis, and 10 GSLs among them were identified as glucoraphanin, sinigrin, glucoalyssin, diglucothiobeinin, glucobrassicanapin, glucoerucin, glucobrassicin, dimeric, 4-mercaptobutyl GSL, 4-methoxy glucobrassicin, gluconasturttin by using LC-APCI-MS analysis, but 7 compounds were not identified. (i) The total GSL content in seed sprouts initially increased up to 3 DAS and then decreased according to their seedling growth. In particular, glucoraphanin known as a strong anti-cancer reagent was found the highest level (5.05 ${\mu}mol/g$ dry wt.) at 3 DAS. The most abundant GSL was glucoerucin ranged from 26.0~49.6 ${\mu}mol/g$ dry wt. (ii) In the time-course, the total GSL contents increased dramatically from 3-week (5.91 ${\mu}mol/g$ dry wt.) to 7-week after sowing (32.2 ${\mu}mol/g$ dry wt.). The major GSLs were glucoraphanin, glucoerucin and 4-methoxy glucobrassicin. (iii) By comparing GSL contents with five different cultivars, the total GSL contents increased from 4-week to 6-week after sowing, regardless of cultivar. In 4-week-old, the order with the total GSL content was "Rucola" > "Rocket Herbs" ${\geqq}$ "Odyssey" > "Takii" > "Herb", but in 6-week-old it is changed as "Takii" > "Herb" > "Odyssey" > "Rucola" > "Rocket Herbs" even there was almost no significantly difference between them.

Keywords

References

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