농업과학연구 (Korean Journal of Agricultural Science)

  • 제38권1호
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  • Pages.51-63
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  • 2011
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  • 2466-2402(pISSN)
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  • 2466-2410(eISSN)
충남대학교 농업과학연구소 (Institute of Agricultural Science, CNU)
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홀스타인종과 한우에 있어서 BoLA-DRB3 유전자의 단일염기다형과 반수체 분석

Analyses of single nucleotide polymorphisms and haplotypes of BoLA-DRB3 gene in Holstein and Hanwoo

  • 정행진 (충남대학교 원예학과) ;
  • 유성란 (충남대학교 동물바이오시스템과학과) ;
  • 라세둘 (충남대학교 동물바이오시스템과학과) ;
  • 이준헌 (충남대학교 동물바이오시스템과학과) ;
  • 도창희 (충남대학교 원예학과) ;
  • 류승희 (충청남도 축산기술연구소) ;
  • 상병찬 (충남대학교 원예학과)
  • Jeong, Hang-Jin (Dept. of Animal Biosystem Science, Chungnam National University) ;
  • Yu, Seong-Lan (Dept. of Animal Science and Biotechnology, Chungnam National University) ;
  • Hoque, M.R. (Dept. of Animal Science and Biotechnology, Chungnam National University) ;
  • Lee, Jun-Heon (Dept. of Animal Science and Biotechnology, Chungnam National University) ;
  • Do, Chang-Hee (Dept. of Animal Biosystem Science, Chungnam National University) ;
  • Ryoo, Seung-Heui (Government of Changcheongnam-Do, Livestock Research Institute) ;
  • Sang, Byung-Chan (Dept. of Animal Biosystem Science, Chungnam National University)
  • 투고 : 2011.02.10
  • 심사 : 2011.03.09
  • 발행 : 2011.03.30
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초록

BoLA (bovine leukocyte antigens) have been known as gene complex related with bovine diseases and immunological traits. This study was conducted to find out the characteristics of BoLA-DRB3 gene related to mastitis and BL(bovine leukocyte) from 280 cattle [193 animals of Holstein cattle and 87 animals of Hanwoo]. As a result, five PCR-RFLP types (b, d, e, f and g) using HaeIII restriction enzyme, three BstYI restriction patterns (b, d and e) and eight RsaI restriction types(b, d, f, I, j, n, o and w) were identified. Moreover, we identified new d' type ($197{\rightarrow}175$/22), having one more cutting site by BstYI enzyme than d type allele and n' type ($180{\rightarrow}169$/11) having one more cutting site by RsaI enzyme than n allele was additionally identified. Next, we identified 53 SNPs in BoLA-DRB3 exon2 from 280 cattle. SNP frequency and heterozygosity of Holstein and Hanwoo were investigated in all the SNP genotype. These results might be based on research for identifying marker associated with bovine diseases.

키워드

참고문헌

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