Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Monitoring of Microorganisms in Commercial Liquid Pig Manures in Korea

국내 유통 돈분 액비의 미생물 함량 모니터링

  • Lim, Seong-Mook (Chemical Safety Division, National Academy of Agricultural Science, RDA) ;
  • Lee, Ji-Ho (Chemical Safety Division, National Academy of Agricultural Science, RDA) ;
  • Go, Woo-Ri (Chemical Safety Division, National Academy of Agricultural Science, RDA) ;
  • Kunhikrishnan, Anitha (Chemical Safety Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Won-Il (Chemical Safety Division, National Academy of Agricultural Science, RDA)
  • 임성묵 (국립농업과학원 유해화학과) ;
  • 이지호 (국립농업과학원 유해화학과) ;
  • 고우리 (국립농업과학원 유해화학과) ;
  • ;
  • 김원일 (국립농업과학원 유해화학과)
  • Received : 2011.11.24
  • Accepted : 2011.12.16
  • Published : 2011.12.31
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Abstract

Total aerobic bacteria, Esherichia coli O157:H7, and Salmonella spp. were examined in commercial liquid pig manures. Commercial liquid pig manures (n=33) were collected from muck joint resource recovery plant at April, June, August, October 2009, Korea. Total aerobic bacteria were incubated at $37^{\circ}C$ for 24-48 hrs, and quantified as a colony-forming unit (CFU) $mL^{-1}$. Analysis of Esherichia coli O157:H7 and Salmonella spp. were followed by Korean Food Standards Codex method. Colony of Salmonella spp. was confirmed by API kit and real time polymerase chain reaction (PCR). Total aerobic bacteria isolated from fermented commercial liquid pig manures ranged from 2.8 to $24.3{\times}10^4\;CFU\;mL^{-1}$. Esherichia coli O157:H7 was not detected, and Salmonella spp. showed the low detection frequency at only 1 sample. This study suggests that continuous monitoring in commercial liquid pig manures is required to improve the agricultural food through management of agricultural land contaminated with liquid pig manures.

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References

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