The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Investigation of Nonylphenols Contamination in Solvents and Solid-phase Extraction Cartridge, and its Removal Protocols

정밀분석용 용매 및 SPE의 Nonylphenols 오염평가 및 제거

  • Park, Jong-Min (Division of Chemical Safety, National Academy of Agricultural Science, RDA) ;
  • Choi, Geun-Hyonng (Division of Chemical Safety, National Academy of Agricultural Science, RDA) ;
  • Kim, Jung-Im (Division of Chemical Safety, National Academy of Agricultural Science, RDA) ;
  • Hong, Su-Myeong (Division of Chemical Safety, National Academy of Agricultural Science, RDA) ;
  • Kwon, Oh-Kyung (Division of Chemical Safety, National Academy of Agricultural Science, RDA) ;
  • Im, Geon-Jae (Division of Chemical Safety, National Academy of Agricultural Science, RDA) ;
  • Kim, Jin-Hyo (Division of Chemical Safety, National Academy of Agricultural Science, RDA)
  • 박종민 (농촌진흥청 국립농업과학원 유해화학과) ;
  • 최근형 (농촌진흥청 국립농업과학원 유해화학과) ;
  • 김정임 (농촌진흥청 국립농업과학원 유해화학과) ;
  • 홍수명 (농촌진흥청 국립농업과학원 유해화학과) ;
  • 권오경 (농촌진흥청 국립농업과학원 유해화학과) ;
  • 임건재 (농촌진흥청 국립농업과학원 유해화학과) ;
  • 김진효 (농촌진흥청 국립농업과학원 유해화학과)
  • Received : 2011.02.17
  • Accepted : 2011.03.16
  • Published : 2011.03.31
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Abstract

Nonylphenols are toxic compounds classified as endocrine disruptors. We investigated the nonylphenols clean-up procedures for the contamination control in the quantitative analysis. In this research we analyzed the residual nonylphenols in the solvent and the SPE cartridges. First, at the analysis of HPLC grade solvents (n-hexane, diethyl ether, ethyl acetate and its mixture), diethyl ether was confirmed the residue as 0.963 ${\mu}g/mL$, and we eliminated the contaminant through the distillation with $CaH_2$, Second, at the analysis of SPE cartridges (silica gel and Florisil), all products were showed the residue at 0.046~13.0 ${\mu}g/mL$, but unfortunately the residue in the cartridge were not easily removed with referenced methods in all tested SPE cartridges except in silica gel SPE cartridge with glass ware.

계면활성제인 4-alkylphenol polyethoxylate는 한국 등 여러 국가에서 매우 광범위하계 사용되어 왔으나 이들 화합물 및 분해산물이 최근 내분비계에 장애를 일으키는 환경호르몬 물질로 분류됨에 따라 농약 등의 첨가물로 사용가능한 nonylphenols(NPs)에 대한 잔류 연구가 매우 활발하게 진행되고 있다. 본 연구에서는 극미량 정밀 잔류분석기술이 필요한 nonylphenols에 대하여 분석용매 몇 정제용 카트리지 선정에 필요한 전처리기술에 대해 보고하고자 한다. 분석 결과에 따르면, HPLC등급의 용매이라 할지라도 $Et_2O$의 경우 100배 농축 용매에서 NPs가 검출되어 시료 분석 시 방해요인으로 작용하였으며, 이의 제거를 위해 $CaH_2$를 사용한 증류 후 사용법을 개발하였다. 또한, 시료정제용 카트리지는 silica gel 및 Florisil 제품군, 그리고 glass ware과 plastic ware로 나누어 카트리지 내 nonylphenols 잔류량을 분석한 결과 silica gel 제품군 glass ware 제품이 전처리 정제용으로 적합한 것으로 확인되었다.

Keywords

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