미생물학회지 (Korean Journal of Microbiology)

  • 제47권2호
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  • Pages.170-173
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  • 2011
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Sorangium cellulosum 균주들의 에포틸론 생합성 유전자 보존

Conservation of the Epothilone-Biosynthetic Genes in Sorangium cellulosum Strains

  • 현혜숙 (호서대학교 생명공학과 점액세균은행) ;
  • 윤진권 (호서대학교 생명공학과 점액세균은행) ;
  • 조경연 (호서대학교 생명공학과 점액세균은행)
  • Hyun, Hye-Sook (Myxobacteria Bank, Department of Biotechnology, Hoseo University) ;
  • Youn, Jin-Kwon (Myxobacteria Bank, Department of Biotechnology, Hoseo University) ;
  • Cho, Kung-Yun (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
  • 투고 : 2011.06.13
  • 심사 : 2011.06.21
  • 발행 : 2011.06.30
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초록

국내에서 분리된 에포틸론 생산 점액세균 Sorangium cellulosum KYC3013의 에포틸론 생합성 유전자군(epoA~F, epoK)을 클로닝하였다. 이 유전자들이 암호화하고 있는 단백질들의 아미노산 서열을 다른 대륙 또는 나라에서 분리된 S. cellulosum SMP44, S. cellulosum So ce90, S. cellulosum So0157-2의 단백질들과 비교한 결과 서로 97.4-99.8% 동일하였다. 이러한 결과는 에포틸론 생합성 유전자들이 S. cellulosum 균주들 사이에서 잘 보존되어 있음을 보여주었다.

The epothilone biosynthetic gene cluster (epoA~F, epoK) of Sorangium cellulosum KYC3013, an epothilone producing myxobacterium isolated in Korea, was cloned. When the amino acid sequences of the encoded proteins were compared with those from S. cellulosum SMP44, S. cellulosum So ce90, and S. cellulosum So0157-2, which were isolated in other continents or country, the proteins from different strains were 97.4-99.8% identical each other. This suggested that the epothilone-biosynthetic gene clusters are well conserved in S. cellulosum strains.

키워드

참고문헌

  1. Bollag, D.M., P.A. McQueney, J. Zhu, O. Hensens, L. Koupal, J. Liesch, M. Goetz, E. Lazarides, and C.M. Woods. 1995. Epothilones, a new class of microtubule-stabilizing agents with a taxol-like mechanism of action. Cancer Res. 55, 2325-2333.
  2. Cheng, K.L., T. Bradley, and D.R. Budman. 2008. Novel microtubule-targeting agents - the epothilones. Biologics. 2, 789-811.
  3. Gerth, K., N. Bedorf, G. Hofle, H. Irschik, and H. Reichenbach. 1996. Epothilones A and B: antifungal and cytotoxic compounds from Sorangium cellulosum (Myxobacteria). production, physicochemical and biological properties. J. Antibiot. 49, 560-563. https://doi.org/10.7164/antibiotics.49.560
  4. Gong, G.L., X. Sun, X.L. Liu, W. Hu, W.R. Cao, H. Liu, W.F. Liu, and Y.Z. Li. 2007. Mutation and a high-throughput screening method for improving the production of epothilones of Sorangium. J. Ind. Microbiol. Biotechnol. 34, 615-623. https://doi.org/10.1007/s10295-007-0236-2
  5. Hardt, I.H., H. Steinmetz, K. Gerth, F. Sasse, H. Reichenbach, and G. Hofle. 2001. New natural epothilones from Sorangium cellulosum, strains So ce90/B2 and So ce90/D13: isolation, structure elucidation, and SAR studies. J. Nat. Prod. 64, 847-856 https://doi.org/10.1021/np000629f
  6. Hofle, G., N. Bedorf, K. Gerth, and H. Reichenback. 1993. Patent DE 4138042.
  7. Hyun, H., J. Chung, J. Kim, J.S. Lee, B.M. Kwon, K.H. Son, and K. Cho. 2008. Isolation of Sorangium cellulosum carrying epothilone gene clusters. J. Microbiol. Biotechnol. 18, 1416-1422.
  8. Julien, B., S. Shah, R. Ziermann, R. Goldman, L. Katz, and C. Khosla. 2000. Isolation and characterization of the epothilone biosynthetic gene cluster from Sorangium cellulosum. Gene 16, 153-160.
  9. Lee, C., H. Hyun, and K. Cho. 2009. Isolation of myxobacteria carrying soraphen biosynthetic gene clusters. Korean J. Microbiol. Biotechnol. 37, 10-16.
  10. Lee, H., J. Youn, and K. Cho. 2011. Phylogenetic analysis of Sorangium cellulosum strains based on cellulase gene sequences. Korean J. Microbiol. Biotechnol. 39, 20-28.
  11. Molnar, I., T. Schupp, M. Ono, R. Zirkle, M. Milnamow, B. Nowak-Thompson, N. Engel, C. Toupet, A. Stratmann, D.D. Cyr, and et al. 2000. The biosynthetic gene cluster for the microtubule- stabilizing agents epothilones A and B from Sorangium cellulosum So ce90. Chem. Biol. 7, 97-109. https://doi.org/10.1016/S1074-5521(00)00075-2
  12. Schneiker, S., O. Perlova, O. Kaiser, K. Gerth, A. Alici, M.O. Altmeyer, D. Bartels, T. Bekel, S. Beyer, E. Bode, and et al. 2007. Complete genome sequence of the myxobacterium Sorangium cellulosum. Nat. Biotechnol. 25, 1281-1289. https://doi.org/10.1038/nbt1354
  13. Stein, A. 2010. Ixabepilone. Clin. J. Oncol. Nurs. 14, 65-71. https://doi.org/10.1188/10.CJON.65-71