대한한방부인과학회지 (The Journal of Korean Obstetrics and Gynecology)

  • 제24권2호
  • /
  • Pages.52-67
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  • 2011
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  • 1229-4292(pISSN)
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  • 2508-3619(eISSN)
대한한방부인과학회 (The Society of Korean Medicine Obstetrics and Gynecology)
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조경종옥탕(調經種玉湯)이 Estradiol Valerate로 유발된 다낭성 난소 모델에서 난포의 성숙 및 NGF 발현에 미치는 영향(影響)

Effects of J okyeongjongok-Tang on the Maturation of Follicles and NGF Expression in Rats with Estradiol Valerate-induced Polycystic Ovaries

  • 양준모 (세명대학교 한의과대학 한방부인과교실) ;
  • 서일복 (세명대학교 한의과대학 한방부인과교실) ;
  • 이동녕 (세명대학교 한의과대학 한방부인과교실) ;
  • 김형준 (세명대학교 한의과대학 한방부인과교실)
  • Yang, Joon-Mo (Dept. of Oriental Gynecology, College of Oriental Medicine, Se-Myung University) ;
  • Seo, Il-Bock (Dept. of Oriental Gynecology, College of Oriental Medicine, Se-Myung University) ;
  • Lee, Dong-Nyung (Dept. of Oriental Gynecology, College of Oriental Medicine, Se-Myung University) ;
  • Kim, Hyung-Jun (Dept. of Oriental Gynecology, College of Oriental Medicine, Se-Myung University)
  • 투고 : 2011.04.30
  • 심사 : 2011.05.11
  • 발행 : 2011.05.28
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초록

Objectives: This study was designed to investigate the effects of Jokyeongjongok -Tang(JJT) on the progression of the estradiol valerate(EV)-induced polycystic ovaries(PCO) in rats. Methods: PCO was induced by single intramuscular injection with estradiol valerate(EV)(4 mg) in female rats. Normal group(n=8) were injected with sesame oil and orally administrated distilled water for sixty days. PCO control group (n=8) were injected with EV and orally administrated distilled water for sixty days. JJT treated group(n=8) were injected with EV and orally administrated JJT for same duration. At the end day of experiment, we measured weights of body, ovaries, adrenal glands and uterus. The histopathological changes of ovaries were also evaluated. And we observed the NGF and CRF expression by immunohistochemistry. Results: The results were as follows - The weights(mg) of ovaries of JJT treated group($58.4{\pm}9.4$) were significantly increased(p<0.01) compared with PCO control group($42.3{\pm}8.5$). - The numbers of mature follicles of JJT treated group($10.1{\pm}2.5$) were significantly increased(p<0.01) compared with PCO control group($6.1{\pm}2.1$). - The numbers of cystic follicles of JJT treated group($1.8{\pm}1.4$) were significantly decreased(p<0.05) compared with PCO control group($3.8{\pm}1.5$). - The expressions of NGF-immunoreactive cells in the ovaries of JJT treated group were weaker than PCO control group. Conclusions: From the above results, we concluded that Jokyeongjongok-Tang (JJT) contributes to a normal maturation of follicles and has the effects of promoting a normal ovulation. And these effects may be related with the decreased NGF activities in the ovaries.

키워드

참고문헌

  1. Dunaif A et al. Current issues in endocrinology and metabolism: polycystic ovary syndrome. Cambridge: Blackwell Scientific Co. 1992;27:347-58.
  2. Stein IF, Leventhal ML. Amenorrhea associated with bilateral polycystic ovaries. Am J Obstet Gynecol. 1935; 29:181-91. https://doi.org/10.1016/S0002-9378(15)30642-6
  3. 민응기. 다낭성 난소 증후군. 대한산부회지. 2008;51(8):805.
  4. 金曈一, 尹鐘元, 李泰均. 多囊性卵巢症候群에 關한 文獻的考察. 大韓韓方婦人科學會誌. 1997;10(1):73-91.
  5. 尹素英, 姜明子. 多囊性卵巢症候群患者의 韓方治療에 대한 臨床的考察. 大韓韓方婦人科學會誌. 2000;13(2):437-51.
  6. 陳貴廷, 楊思澍. 實用中西醫結合診斷治療學. 北京:中國醫學科技出版社. 1991 :1004-9.
  7. 張鳳嬋, 薛耀. 中醫藥治療多囊卵巢綜合症方法初探. 新中醫. 1991;1:14-6.
  8. 周羽珍. 蔡小蓀治療婦科痼疾驗方拾萃. 江蘇中醫. 1996;17(1):9-10.
  9. 吳桂芳, 李祥云. 中醫藥治療多囊卵巢綜合症硏究槪況. 河南中醫. 1994;14(2) :123-5.
  10. 金維新. 不孕症的診斷與中醫治療. 北京科學出版社. 1992;172-7.
  11. 施令義. 中西醫結合硏究學術論文編. 婦産科專緝. 1983;10:32.
  12. 許俊. 東醫寶鑑. 서울:南山堂. 1991; 603-5.
  13. 韓醫科大學本草學編纂委員會. 本草學. 서울:永林社. 2000:124, 136, 193-4, 302-3, 336-8, 347-8, 354-5, 405-9, 413-4, 578-82.
  14. Brawer JR, Munoz M, Farookhi R. Development of the polycystic ovarian condition(PCO) in the estradiol valerate -treated rat. Biol Reprod. 1986;35 :647-55. https://doi.org/10.1095/biolreprod35.3.647
  15. 김정훈. 다낭성난소증후군 치료의 최신지견. 대한산부인과학회지. 2005;48(8) :1851-69.
  16. 羅元愷. 實用中醫婦科學. 上海:上海科學技術出版社. 1994;262, 271-5.
  17. 陳玲, 王明英, 王君. 健脾益腎化痰湯治療多囊卵巢綜合症30例. 江蘇中醫. 1997;7:31.
  18. 周羽珍. 蔡小蓀治療婦科痼疾驗方拾萃. 江蘇中醫. 1999;7:31.
  19. 양승정 등. 蒼附導痰湯이 estradiol valerate로 유발된 백서의 다낭성 난소에 미치는 影響. 大韓韓方婦人科學會誌. 2002;15(2):1-11.
  20. 남은정 등. 定經湯이 estradiol valerate로 유도된 흰쥐의 다낭성 난소에 미치는 影響. 大韓韓方婦人科學會誌. 2007; 20(1):99-113.
  21. 유영기등. 腎氣丸合蒼附導痰湯이estradiol valerate로 유도된 흰쥐의 다낭성 난소에 미치는 影響. 大韓韓方婦人科學會誌. 2007;20(1):84-98.
  22. 김희주, 김윤상, 임은미. 蒼附六君湯 이 estradiol valerate로 유도된 흰쥐의 다낭성 난소에 미치는 影響. 大韓 韓方婦人科學會誌. 2008;21(2):1-16.
  23. 이연경 등. 歸脾湯이 estradiol valerate로 유도된 흰쥐의 다낭성 난소에 미치는 影響. 大韓韓方婦人科學會誌. 2008; 21(3):60-74.
  24. 윤문희 등. 開鬱二陳湯이 estradiol valerate로 유도된 흰쥐의 다낭성 난소 발달에 미치는 影響. 大韓韓方婦人科學會誌. 2010;23(2):1-19.
  25. 구희준, 조성희. 皂角刺가 estradiol valerate로 유발된 白鼠의 다낭성 난소에 미치는 影響. 大韓韓方婦人科學會誌. 2010;23(2):71-84.
  26. 梁東仙등. 香附子가 estradiol valerate로 유발된 백서의 다낭성 난소에 미치는 影響. 東新大學校大學院碩士學位論文. 2010.
  27. 金性銓. 調經種玉湯의 抽出方法에 따른 家免의 卵巢變化에 미치는 영향. 慶熙韓醫大論文集. 1978;1:69-73.
  28. 張榮姬, 林動潤. 調經種玉湯의 藥理作用에 關한 硏究. 朝鮮醫大論文集. 1984;9(1):193-206.
  29. 宋柄基, 李京變. 調經種玉湯이 卵巢機能에 미치는 影響. 慶熙韓醫大論文集. 1983;6:139-51.
  30. 黃度淵原著. 辛民敎, 永林社編輯部編著. 新增方藥合編. 서울:永林社. 2002:157-8.
  31. Barbier RL, Ryan KJ. Hyperandrogenism, insulin resistance, and acanthosis nigricans syndrome : a common endocrinopathy with distinct pathophysiologic features. Am J Obstet Gynecol. 1983;147:90-4. https://doi.org/10.1016/0002-9378(83)90091-1
  32. Goldzeiher JW, Axelrod LR. Clinical and biochemical features of polycystic ovarian disease. Fertil Steril. 1963;14 :631-3. https://doi.org/10.1016/S0015-0282(16)35047-6
  33. 구윤희, 나용진. 다낭성난소증후군 환자의 불임치료. 대한산부인과학회지. 2010;53(10):869-80.
  34. Quirarte GL et al. Estradiol Valerate and alcohol intake : dose-response assessments. BMC Pharmacol. 2007 ;7:3.
  35. Stener VE et al. Rats with steroid -induced polycystic ovaries develop hypertension and increased sympathetic nervous system activity. Reprod Biol Endocrinol. 2005;3:44. https://doi.org/10.1186/1477-7827-3-44
  36. 김탁. 다낭성 난소 증후군의 최신 지견. 대한산부인과학회. 연수강좌. 2002; 31:38-44.
  37. Levi MR. Angrlrtti PU. Nerve growth factor. Physiol Rev. 1968;48:534-69.
  38. Levi MR. The nerve growth factor 35 years later. Science. 1987;237: 1154-62. https://doi.org/10.1126/science.3306916
  39. Dissen GA et al. A role for trkA nerve growth factor receptors in mammalian ovulation. Endocrinology. 1996;137(1):198-209. https://doi.org/10.1210/en.137.1.198
  40. Dissen GA et al. Intraovarian excess of nerve growth factor increase androgen secretion and disrubts estrous cyclicity in the rat. Endocrinology. 2000;141(3) :1073-82. https://doi.org/10.1210/en.141.3.1073
  41. Dyer CA, Erickson GF. Norepinephrine amplifies human chorionic gonadotropin -stimulated androgen biosynthesis by ovarian theca-interstitial cells. Endocrinology. 1985;116(4):1645-52. https://doi.org/10.1210/endo-116-4-1645
  42. Nestler EJ et al. Chronic antidepressant administration decreases the expression of tyrosine hydroxylase in the rat locus coeruleus. Proc Natl Acad Sci USA. 1990;87:7522-6. https://doi.org/10.1073/pnas.87.19.7522
  43. Monnikes H et al. CRF in the paraventricular nucleus mediate gastric and colonic motor response to restraint stress. Am J Physiol. 1992;262:G137-43.

피인용 문헌

  1. 여성불임환자 6례의 임상보고 vol.27, pp.2, 2011, https://doi.org/10.15204/jkobgy.2014.27.2.103