The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Effects of Electroacupuncture on Immobilization Stress Responses : A Study on Inhibitory Avoidance Task, Forced Swimming Test, and Stress Hormones

전기침이 결박 스트레스 반응에 미치는 영향 : 억제성 회피 과제, 강제 수영 시험, 스트레스 호르몬 반응 연구

  • Kwon, So-Yeon (Dept. of Internal medicine, College of Oriental medicine Won-Kwang) ;
  • Kim, Min-Soo (Dept. of Statistics, Chonnam National University) ;
  • Lee, Sang-Kwan (Dept. of Internal medicine, College of Oriental medicine Won-Kwang) ;
  • Je, Jun-Tae (Dept. of Internal medicine, College of Oriental medicine Won-Kwang) ;
  • Oh, Jae-Gun (Dept. of Internal medicine, College of Oriental medicine Won-Kwang) ;
  • Lee, Jong-Deok (Dept. of Internal medicine, College of Oriental medicine Won-Kwang) ;
  • Sung, Kang-Keyng (Dept. of Internal medicine, College of Oriental medicine Won-Kwang)
  • 권소연 (원광대학교 한의과대학 심계내과학 교실) ;
  • 김민수 (전남대학교 통계학과) ;
  • 이상관 (원광대학교 한의과대학 심계내과학 교실) ;
  • 제준태 (원광대학교 한의과대학 심계내과학 교실) ;
  • 오재건 (원광대학교 한의과대학 심계내과학 교실) ;
  • 이종덕 (원광대학교 한의과대학 심계내과학 교실) ;
  • 성강경 (원광대학교 한의과대학 심계내과학 교실)
  • Published : 2011.03.30
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Abstract

Objectives : This study investigated the effects of electroacupuncture on memory, depression, and plasma stress hormone levels in rats that were under immobilization stress. Materials and Methods : The immobilization-only group was given two hours of immobilization stress for 10 consecutive days. The immobilization and high frequency EA group was given two hours of immobilization stress simultaneously with high frequency (100Hz) electroacupuncture stimulation on the right ST-36 (Zusanli) for 10 consecutive days. We conducted the inhibitory avoidance and forced swimming tests to recognize whether immobilization stress and electroacupuncture have effects on memory and depression. We collected blood samples from the tail of each rat at 30, 60, 90, and 120-minute intervals during the immobilization stress and EA stimulation to measure plasma concentrations of ACTH, corticosterone, melatonin, and norepinephrine induced by immobilization stress and electroacupuncture. Results : There was a significant effect of high frequency on the increase in anamnesis based on the result of the inhibitory avoidance test, but there was no significant effect of decreasing depression based on the result of the forced swim test. Also, there was no significant effect on the response indicated by stress hormones. Conclusions : 1. High frequency electroacupuncture (100Hz) improved anamnesis in immobilization stress states under the inhibitory avoidance task. 2. High frequency electroacupuncture (100Hz) did not reduce depression induced by immobilization stress under the forced swimming test. 3. High frequency electroacupuncture (100Hz) did not decrease stress hormones through blood sampling.

목적 : 본 연구는 결박 스트레스를 시행한 백서의 기억력, 우울, 스트레스 호르몬의 혈중 농도에 대한 전기침의 효과를 관찰하였다. 재료와 방법 : 결박 그룹은 10일 동안 2시간의 결박 스트레스를 받았다. 결박 스트레스 및 고주파수 전침 그룹은 2시간의 결박 스트레스를 받는 동시에 100Hz의 전침을 우측 족삼리 (ST-36)에 시행하였다. 기억력과 우울에 미치는 결박 스트레스와 침의 효과를 비교하기 위하여 억제성 회피 과제와 강제 수영 검사를 실시하였다. 결박 스트레스 및 전기침으로 유도된 코르티코스테론과 멜라토닌, 부신피질자극호르몬, 노르아드레날린의 농도를 측정하기 위하여 결박 스트레스 및 전기침 자극이 시작된 30분, 60분, 90분, 120분 후에 백서의 꼬리에서 혈액 샘플을 채취하였다. 결과 : 억제성 회피 과제에서 전기침을 시행한 그룹은 시행하지 않은 그룹에 대해서 장기 기억의 증진에 유의한 변화가 있었다. 강제 수영 검사 결과에서는 우울에 유의한 변화가 없었으며, 스트레스 호르몬 반응 결과에서도 유의한 변화가 없었다. 결론 : 1. 고주파수 전기침 (100Hz)은 억제성 회피 과제 결과 결박 스트레스에 기억력을 상승시켰다. 2. 고주파수 전기침 (100Hz)은 강제 수영 시험 결과 결박 스트레스로 유도된 우울을 감소시키지 않았다. 3. 고주파수 전기침 (100Hz)은 혈액 채취 결과 스트레스 호르몬을 감소시키지 않았다.

Keywords

References

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