Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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Animal Skin Pigmentation Model Using Full Thickness Skin Graft in C57BL/6 Mouse

C57BL/6 마우스의 등에 시행한 자가 전층피부이식편을 이용한 색소침착 동물모델

  • Lee, Hong-Ki (Image Plastic Surgery Clinic) ;
  • Park, Jong-Lim (Banobagi Plastic and Aesthetic Clinic) ;
  • Heo, Eun-Ju (Department of Plastic and Reconstructive Surgery, Seoul National University College of Medicine) ;
  • Kim, Suk-Wha (Department of Plastic and Reconstructive Surgery, Seoul National University College of Medicine)
  • 이홍기 (이미지 성형외과) ;
  • 박종림 (바노바기 성형외과) ;
  • 허은주 (서울대학교 의과대학 성형외과학교실) ;
  • 김석화 (서울대학교 의과대학 성형외과학교실)
  • Received : 2010.01.08
  • Accepted : 2011.06.21
  • Published : 2011.11.10
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Abstract

Purpose: Skin grafting is one of the most commonly used methods in reconstructive plastic surgery field, but complications such as color change, contracture or hypertrophy are common problems. However, pathophysiology of the color change after skin graft is not yet determined and no animal model is established. Methods: Full thickness skin grafts were performed on the dorsum of C57BL/6 mice. Serial chronological gross inspection for color change and pigmentation were examined. Melanin pigments were traced by Fontana-Masson staining and semi-quantitative analysis was performed. In addition, immunohistochemical staining of S-100, Micropthalmia related Transcription Factor (MITF) and Melan-A antibodies were also performed to observe melanocytes and their changes. Results: After skin graft, color change and pigment spots were observed in the graft. Fontana-Masson staining showed melanin pigments in the epidermal and dermal layers in all mice. Immunohistochemistry staining to S-100, MITF, Melan-A antibodies showed melanocytes at the basal layer of epidermis and dermis. Conclusion: In conclusion, we have established an animal model for skin pigmentation after skin graft. We believe this study may be useful in understanding of the behavior of melanocytes after skin graft.

Keywords

References

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