Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Antibacterial Effect of Photodynamic Therapy using Photogem and a 632 nm Diode Laser on $Helicobacter$ $pylori$

  • Ahn, Jin-Chul (Medical Laser Research Center, Dankook University) ;
  • Shin, Jang-In (Medical Laser Research Center, Dankook University) ;
  • Chang, So-Young (Medical Laser Research Center, Dankook University) ;
  • Kim, Hong-Ja (Department of Internal Medicine, Dankook University) ;
  • Hong, Seong-No (Department of Clinical Laboratory Science, Dongnam Health College)
  • Received : 2011.06.21
  • Accepted : 2011.12.13
  • Published : 2011.12.31
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Abstract

Eradication of $H.$ $pylori$, usually carried out by using antimicrobial agents, is essential for preventing gastric ulcers and cancers. The $H.$ $pylori$ isolates, however, have continuously grown antimicrobial resistance, which have caused difficulty in treating the bacteria and in turn, photodynamic therapy (PDT) has been found to be effective in inducing deaths of variety of bacteria. After PDT treatment, the number of colony forming units (CFU), the morphologic changes, and flow cytometry were observed. In the PDT group containing 100 and 200 ${\mu}g$/ml photogem, no live $H.$ $pylori$ was observed, while 10 and 50 ${\mu}g$/ml photogem were only partially effective. $H.$ $pylori$ of the PDT group also displayed distortion and shrinkage in morphology. This study demonstrated that photogem-mediated PDT effectively induces deaths of $H.$ $pylori$.

Keywords

References

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