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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Screening of Functional Materials from Solvent Fractions of Apple Flower Leaf Extract

사과꽃잎 추출물의 용매 분획으로부터 기능성 소재의 탐색

  • Choi, Sun-Ju (Department of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University) ;
  • Cho, Eun-Ah (Department of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University) ;
  • Cho, Eun-Hye (Department of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University) ;
  • Jeong, Yoon-Joo (R&D Center, Cotde Co., Ltd.) ;
  • Ku, Chang-Sub (R&D Center, Cotde Co., Ltd.) ;
  • Ha, Byung-Jhip (R&D Center, Cotde Co., Ltd.) ;
  • Chae, Hee-Jeong (Department of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University)
  • 최선주 (호서대학교 식품생물공학과 및 식품기능안전연구센터) ;
  • 조은아 (호서대학교 식품생물공학과 및 식품기능안전연구센터) ;
  • 조은혜 (호서대학교 식품생물공학과 및 식품기능안전연구센터) ;
  • 정윤주 ((주)콧데 기업부설연구소) ;
  • 구창섭 ((주)콧데 기업부설연구소) ;
  • 하병집 ((주)콧데 기업부설연구소) ;
  • 채희정 (호서대학교 식품생물공학과 및 식품기능안전연구센터)
  • Received : 2011.03.10
  • Accepted : 2011.04.25
  • Published : 2011.04.30
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Abstract

Fractional solvent extraction by organic solvents such as hexane, chloroform, ethylacetate, and butanol was carried out using 70% ethanol extract of apple flower leaves. Biological activities including antioxidant, whitening, antimicrobial and anti-wrinkle activities were investigated and bio-active materials of the extracts were identified using GC/MSD. Among the tested solvent fractions, ethylacetate fraction showed the highest total polyphenol content (1218.94 ${\mu}g/mL$), and flavonoid (140 ${\mu}g/mL$). The DPPH radical scavenging activities was over 80% at a dry matterbased concentration of 200 ${\mu}g/{\mu}L$ and SOD-like activity was over 90% at 50 ${\mu}g/mL$ concentration in ethylacetate fraction that was slightly lower than of ascorbic aicd. Tyrosinase inhibition activity related to skin-whitening was over 60% by ethylacetate fraction of 100 ${\mu}g/mL$. As an anti-aging effect, elastase inhibitory activity was about 45% in ethylacetate fraction. Also, it showed a significantly antimicrobial activity against P. acenes. From GC/MSD analysis, a characteristic peak of high content in ethylacetate fraction was identified as kaempferol, which has been reported as a bioactive compound.

Keywords

References

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