Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Development of Probiotic Dairy Product for the Normalization of Microbial Flora in Korean Infants

한국인 영유아 장내균총 정상화를 위한 프로바이오틱 유제품의 개발

  • 김민경 (농촌진흥청 국립축산과학원) ;
  • 최아리 (농촌진흥청 국립축산과학원) ;
  • 한기성 (농촌진흥청 국립축산과학원) ;
  • 정석근 (농촌진흥청 국립축산과학원) ;
  • 채현석 (농촌진흥청 국립축산과학원) ;
  • 장애라 (농촌진흥청 국립축산과학원) ;
  • 설국환 (농촌진흥청 국립축산과학원) ;
  • 오미화 (농촌진흥청 국립축산과학원) ;
  • 김동훈 (농촌진흥청 국립축산과학원) ;
  • 함준상 (농촌진흥청 국립축산과학원)
  • Received : 2010.12.15
  • Accepted : 2011.03.30
  • Published : 2011.04.30
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Abstract

This study was conducted to develop an effective probiotic dairy product to normalize the microbial flora in Korean infants. A total of 2,200 colonies were isolated from 25 Korean neonates, and 16S rRNA of 348 isolates was analyzed. Approximately 40% of the lactic-acid producing bacterial isolates were Enterococcus faecalis, and 34.2% of them were strains similar to XR7 in the GenBank database. The fastest growing strain in MRS broth was registered as 91532 by the KACC. The selected strain was freeze-dried and utilized to ferment a milk-containing rice soup, tarakjuk. Microbiological, physico-chemical, and sensory characteristics of the fermented tarakjuk were compared with fermented milk and tarakjuk. E. faecalis KACC 91532 increased from $6.14{\pm}0.19$ to $7.36{\pm}0.13$ Log CFU/mL, and can be useful as a probiotic, as described in the Standards for Functional Health Foods.

유아의 장내 균총 정상화에 유용한 새로운 발효유제품을 개발하고자 본 실험을 수행하였다. 신생아 분변시료에서 2,200균주를 분리하고 348 균주의 16S rRNA를 분석한 결과, 유산균의 37.8%가 E. faecalis로 확인되었다. 가장 많은 유전자형을 나타낸 E. faecalis 중에서 MRS 액체배지에서 가장 생장이 우수한 E. faecalis KACC 91532를 선발하였다. 선발 유산균을 이용하여 전통식품인 타락죽을 발효하고 이화학적, 관능적 특성을 분석하였다. 타락죽의 발효시 E. faecalis KACC 91532는 $6.14{\pm}0.19$에서 $7.36{\pm}0.13$ Log CFU/mL로 증가하여, 섭취량과 E. faecalis가 건강기능식품기준 및 규격에 고시되어 있는 점을 고려시 건강기능식품으로 판매가 가능하며, 특히 유아의 장내 균 총 정상화에 기여할 수 있을 것으로 생각된다.

Keywords

References

  1. Fanaro, S., Chierici, R., Guerrini, P., and Vigi, V. (2003) Intestinal microflora in early infancy: composition and development. Acta Paediatr. Suppl. 91, 48-55.
  2. Kang, D. G., Kang, S. P., Chang, D. H., Kim, S. H., and Yoon, S. S. (2001) Isolation and characterization of Lactobacillus strains isolated from Korean feces. Korean J. Food Sci. Technol. 33, 567-573.
  3. Klevin, G. (2003) Taxonomy, ecology and antibiotic resistance of enterococci from food and gastro-intestinal tract. Int. J. Food Microbiol. 88, 123-131. https://doi.org/10.1016/S0168-1605(03)00175-2
  4. KFDA (Korea Food and Drug Administration) (2008) Standard for Functional Health Foods. KFDA Notice 2008-64.
  5. KDIA (Korea Dairy Industries Association) (2008) Domestic Statistics. Available from: http://www.koreadia.or.kr/statistics/sub04.html. http://www.koreadia. or.kr/statistics/sub11.html Accessed May. 7. 2010.
  6. Lee, S. G., Han, G. S., Jeong, S. G., Oh, M. H., Jang, A., Kim, D. H., Bae, I. H., and Ham, J. S. (2010) A study on the sensory characteristics of yogurt and antimicrobial activity of Lactobacillus plantarum LHC52 isolated from Kimchi. Korean J. Food Sci. Ani. Resour. 30, 328-335. https://doi.org/10.5851/kosfa.2010.30.2.328
  7. Lee, S. H., Yang, E. H., Kwon, H. S., Kang, J. H., and Kang, B. H. (2008) Potential probiotic properties of Lactobacillus johnsonii IDCC 9203 isolated from infant feces. Korean J. Microbiol. Biotechnol. 36, 121-127.
  8. Mainville, I., Y. Arcand, and E. R. Farnworth. (2005) A dynamic model that simulates the human upper gastrointestinal tract for the study of probiotics. Int. J. Food Microbiol. 99, 287-296. https://doi.org/10.1016/j.ijfoodmicro.2004.08.020
  9. McKinley, M. C. (2005) Effects of probiotics on biotechnological characteristics of yogurt. Int. J. Dairy Technol. 58, 1-12. https://doi.org/10.1111/j.1471-0307.2005.00180.x
  10. Mishra, V. and Prasad, D. N. (2005) Application of in vitro methods for selection of Lactobacillus casei strains as potential probiotics. Int. J. Food. Microbiol. 103, 109-115. https://doi.org/10.1016/j.ijfoodmicro.2004.10.047
  11. Moon, B. Y., Lee, S. K., and Park, J. H. (2006) Antibiotic resistant characteristics of Bifidobacterium from Korean institute origin and commercial yoghurts. Korean J. Food Sci. Technol. 38, 313-316.
  12. Park, J. G., Yun, S. Y., Oh, S, Shin, J. G., and Baek, Y. J. (2003) Probiotic characteristics of Lactobacillus acidophilus KY 1909 isolated from Korean breast-fed infant. Korean J. Food Sci. Technol. 35, 1244-1247.
  13. Penders, J., Thijs, C., Vink, C., Stelma, F. F., Snijders, B., Kummeling, I, van den Brand, P. A., and Stobberingh, E. E. (2009) Factors influencing the composition of the intestinal microbiota in early infancy. Pediatrics 118, 511-521.
  14. Pinto, M. G. V., Franz, C. M. A. P., Schillinger, U., and Holzapfel, W. H. (2006) Lactobacillus sp. with in vitro probiotic properties from human faeces and traditional fermented products. Int. J. Food Microbiol. 109, 205-214. https://doi.org/10.1016/j.ijfoodmicro.2006.01.029
  15. Schillinger, U., Guigas, C., and Holzapfel, W. H. (2005) In vitro adherence and other properties of lactobacilli used in probiotic yoghurt-like products. Int. Dairy J. 15, 1289-1297. https://doi.org/10.1016/j.idairyj.2004.12.008

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