한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제19권2호
  • /
  • Pages.103-110
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  • 2011
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
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초고압 가공 공정을 통한 지치 추출물의 항암 활성 증진

Enhancement of Anticancer Activities from Lithospermum erythrorhizon Extracts by Ultra High Pressure Process

  • 서용창 (의료바이오신소재융복합연구센터 강원대학교 생물소재공학과) ;
  • 최운용 (강원대학교 생물소재공학과) ;
  • 김지선 (의료바이오신소재융복합연구센터 강원대학교 생물소재공학과) ;
  • 조정섭 (두산에코비즈넷) ;
  • 김영옥 (농진청 국립원예특작과학원 인삼특작부) ;
  • 김진철 (강원대학교 생물소재공학과) ;
  • 이현용 (강원대학교 생물소재공학과)
  • Seo, Yong-Chang (Medical & Bio-Material Research Center and Department of Biomaterials Engineering, Kangwon National University) ;
  • Choi, Woon-Yong (Department of Biomaterials Engineering, Kangwon National University) ;
  • Kim, Ji-Seon (Medical & Bio-Material Research Center and Department of Biomaterials Engineering, Kangwon National University) ;
  • Cho, Jeong-Sub (DooSan EcoBizNet) ;
  • Kim, Young-Ock (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Jin-Chul (Department of Biomaterials Engineering, Kangwon National University) ;
  • Lee, Hyeon-Yong (Department of Biomaterials Engineering, Kangwon National University)
  • 투고 : 2010.10.02
  • 심사 : 2011.04.13
  • 발행 : 2011.04.30
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초록

This study was performed to enhance anticancer activities of Lithospermum erythrorhizon by eluting high amount of shikonin through ultra high pressure process. Extraction yield was increased up to 5~10% by ultra high pressure process, compare to the normal extraction processes such as water solvent extraction, 70% ethyl alcohol solvent extraction. The cytotoxicity of the extracts ($1.0{\mu}g/m{\ell}$) from ultra high pressure process was showed the lowest cytotoxicity 13.4% for human lung cell (HEL299). The anticancer activities showed 80~85% by adding $1.0{\mu}g/m{\ell}$ of the extracts from ultra high pressure process in several cancer cell lines such as AGS, Hep3B, MCF-7 and HeLa cells. Among them, MCF-7 cell of the endocrine system was highest inhibited than other cells. The anticancer activities of the extracts from ultra high pressure extraction process showed 10~15%, which was higher than the extracts from normal extraction processes. From HPLC analysis of the extracts, the contents of shikonin in the extracts from ultra high pressure process was 11.42% (w/w), which was 20% higher than others. This results indicate that ultra high pressure process could increase the extraction yield of shikonin and other contents, which resulted in higher anticancer activities.

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참고문헌

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피인용 문헌

  1. Enhancement of Skin Immune Activities of Curcuma longa L. Leaf Extract by Ultra High Pressure Process vol.22, pp.5, 2014, https://doi.org/10.7783/KJMCS.2014.22.5.378
  2. Effect of High Pressure and Steaming Extraction Processes on Ginsenosides Rg3 and Rh2 Contents of Cultured-Root in Wild Ginseng (Panax ginseng C. A. Meyer) vol.20, pp.4, 2012, https://doi.org/10.7783/KJMCS.2012.20.4.270
  3. Cytotoxicity of Methanol Extracts from Cudrania tricuspidata Bureau vol.20, pp.3, 2012, https://doi.org/10.7783/KJMCS.2012.20.3.153
  4. Identification of Fungal Pathogen Causing Seedling Rot of Lithospermum erythrorhizon and Study on the Optimum Growing Temperature for Decreasing of the Seedling Rot vol.21, pp.1, 2013, https://doi.org/10.7783/KJMCS.2013.21.1.27
  5. Protective Effect of Mulberry and Lithospermum erythrorhizon Extracts on Anti-aging against Photodamage vol.42, pp.11, 2013, https://doi.org/10.3746/jkfn.2013.42.11.1744