Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Enhancement of Anticancer Activities from Lithospermum erythrorhizon Extracts by Ultra High Pressure Process

초고압 가공 공정을 통한 지치 추출물의 항암 활성 증진

  • Seo, Yong-Chang (Medical & Bio-Material Research Center and Department of Biomaterials Engineering, Kangwon National University) ;
  • Choi, Woon-Yong (Department of Biomaterials Engineering, Kangwon National University) ;
  • Kim, Ji-Seon (Medical & Bio-Material Research Center and Department of Biomaterials Engineering, Kangwon National University) ;
  • Cho, Jeong-Sub (DooSan EcoBizNet) ;
  • Kim, Young-Ock (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Jin-Chul (Department of Biomaterials Engineering, Kangwon National University) ;
  • Lee, Hyeon-Yong (Department of Biomaterials Engineering, Kangwon National University)
  • 서용창 (의료바이오신소재융복합연구센터 강원대학교 생물소재공학과) ;
  • 최운용 (강원대학교 생물소재공학과) ;
  • 김지선 (의료바이오신소재융복합연구센터 강원대학교 생물소재공학과) ;
  • 조정섭 (두산에코비즈넷) ;
  • 김영옥 (농진청 국립원예특작과학원 인삼특작부) ;
  • 김진철 (강원대학교 생물소재공학과) ;
  • 이현용 (강원대학교 생물소재공학과)
  • Received : 2010.10.02
  • Accepted : 2011.04.13
  • Published : 2011.04.30
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Abstract

This study was performed to enhance anticancer activities of Lithospermum erythrorhizon by eluting high amount of shikonin through ultra high pressure process. Extraction yield was increased up to 5~10% by ultra high pressure process, compare to the normal extraction processes such as water solvent extraction, 70% ethyl alcohol solvent extraction. The cytotoxicity of the extracts ($1.0{\mu}g/m{\ell}$) from ultra high pressure process was showed the lowest cytotoxicity 13.4% for human lung cell (HEL299). The anticancer activities showed 80~85% by adding $1.0{\mu}g/m{\ell}$ of the extracts from ultra high pressure process in several cancer cell lines such as AGS, Hep3B, MCF-7 and HeLa cells. Among them, MCF-7 cell of the endocrine system was highest inhibited than other cells. The anticancer activities of the extracts from ultra high pressure extraction process showed 10~15%, which was higher than the extracts from normal extraction processes. From HPLC analysis of the extracts, the contents of shikonin in the extracts from ultra high pressure process was 11.42% (w/w), which was 20% higher than others. This results indicate that ultra high pressure process could increase the extraction yield of shikonin and other contents, which resulted in higher anticancer activities.

Keywords

References

  1. Bai JH. (2004). Antimicrobial effect of Lithospermum erythorhizon extract on the food-borne pathogens. Korean Journal of Food Science and Technology. 36:823-827.
  2. Cha JY, Jeon BS, Park JW, Moon JC and Cho YS. (2004). Effect of fermented compositions containing Inonotus obliquus with Houttuynia cordata on growth of human AGS Gastric and HCT-15 colon cancer cells. Journal of The Korean Society for Applied Biological Chemistry. 47:202-207.
  3. Chen X, Yang L, Zhang N, Turpin JA, Buckheit RW, Osterling C, Oppenheim JJ and Howard OM. (2003). Shikonin, a component of Chinese herbal medicine, inhibits chemokine receptor function and suppresses human immunodeficiency virus type 1. Antimicrobial Agents and Chemotherapy. 47:2810- 2816. https://doi.org/10.1128/AAC.47.9.2810-2816.2003
  4. Dool R and Peto R. (1981). The causes of cancer : Quantitative estimates of avoidable risks of cancer in the Unitede States today. Journal of the National Cancer Institute. 66:1191-1208.
  5. Jeon YH, Choi SW and Kim MR. (2009). Antimutagenic and cytotoxic activity of ethanol and water extracts from Rubus coreanum. Korean Journal of Food and Cookery Science. 25:379-386.
  6. Jeong HS, Han JG, Ha JH, Kim Y, Oh SH, Kim SS, Jeong MH, Choi GP, Park UY and Lee HY. (2009). Enhancement of anticancer activities of Ephedra sinica, Angelica gigas by ultra high pressure extraction. Korean Journal of Medicinal Crop Science. 17:102-108.
  7. Jo MK and Min KJ. (2007). Anti-microbial activites against oral microbes and growth-inhibitory effect on oral tumor cell of extracts of Perilla and Mugwort. Korean Journal of Environmental Health. 33:115-122. https://doi.org/10.5668/JEHS.2007.33.2.115
  8. Kim CH, Kim HS, Kwon MC, Bae GJ, Ahn JH, Lee HJ, Kang HY and Lee HY. (2006). Increase effect of anticancer activities on Rhodiola sachalinensis A. Bor by the change of extraction process. Korean Journal of Medicinal Crop Science. 14:317-321.
  9. Kim CH, Kwon MC, Han JG, Na CS, Kwak HG, Choi GP, Park UY and Lee HY. (2008). Skin-whitening and UVprotective effects of Angelica gigas Nakai extracts on ultra high pressure extraction process. Korean Journal of Medicinal Crop Science. 16:255-260.
  10. Kim CH, Kwon MC, Syed AQ, Hwang B, Nam JH and Lee HY. (2007). Toxicity reduction and improvement of anticancer activities from Rhodiola sachalinensis A. Bor by ultra high pressure extracts process. Korean Journal of Medicinal Crop Science. 15:411-416.
  11. Lee CB. (2003). Korea Plant Picture book. HyangMoon Press. Seoul, Korea. p. 97-107.
  12. Lee HJ, Lee HJ, Magesh V, Nam DW, Lee EO, Ahn KS, Jung MH, Ahn KS, Kim DK, Kim JY and KIM SH. (2008). Shikonin, acetylshikonin, and isobutyroylshikonin inhibit VEGFinduced angiogenesis and suppress tumor growth in lewis lung carcinoma-breaing Mice. Yakugaku Zasshi. 128:1681-1688. https://doi.org/10.1248/yakushi.128.1681
  13. Lee HK, Kim JS, Kim NY, Kim MJ, Park SU and Yu CY. (2003). Antioxidant, antimutagenicity and anticancer activities of extracts from Cirsium japonicum var. Ussuriense Kitamura. Korean Journal of Medicinal Crop Science. 11:53-61.
  14. Lee KG, Mitchell AE and Shibamoto T. (2000). Determination of antioxidant properties of aroma extracts from various beans. Journal of Agriculture and Food Chemistry 43:4817-4820.
  15. Michael CA, Domnic AS and Ahne M. (1998). Feasibility of drug screening with panels of human tumor cell line using a microculture tetrazolium assay. Cancer Research. 48:589-601.
  16. Park HS, Min KJ, Cha CG, Song JW and Son JC. (2007). Antimicrobial activities against oral microbes and growthinhibitory effect on oral tumor cell by extract of Paeonia lactiflora. Korean Journal of Environmental Health. 33:21-29. https://doi.org/10.5668/JEHS.2007.33.1.021
  17. Park YJ, Cho JY, Kim SH and Heo BG. (2003). Natural dyeing of skeletonizing leaves using dyestuffs extrated from Lithospermum erythorhizon. Korean of Journal Horticultural Science and Technology. 21:422-427.
  18. Sankawa U, Ebizuka Y, Miyazaki T, Isomura Y and Otsuka H. (1977). Antitumor activity of shikonin and its derivatives. Chemical and Pharmaceutical Bulletin. 25:1392-1395.
  19. Sasaki K, Abe H and Yoshizaki F. (2002). In vitro antifungal activity of naphthoquinone derivatives. Biological and Pharmaceutical Bulletin Science. 25:669-670. https://doi.org/10.1248/bpb.25.669
  20. Staniforth V, Wang SY, Shyur LF and Yang NS. (2004). Shikonons, phytocompounds from Lithospermum erythorhizon, inhibit the transcriptional activation of human tumor necrosis factor alpha promoter in vivo. Journal of Biological Chemistry. 279:5877-5885. https://doi.org/10.1074/jbc.M309185200
  21. Yun KJ, Kim DH, Ryu JH and Yook CS. (1999). Studies on the constituents and their antibacterial effect of the root of Lithospermum erythorhizon. Bulletin Kyung Hee Pharmaceutical Science. 27:27-31.

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