Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Effect of Estrogenic Xonibiotics on Boar Sperm Motility and Motion Kinematics

Estrogenic Xenobiotics가 돼지 정자의 운동성 및 운동역학에 미치는 영향

  • Oh, Shin-Ae (Department of Animal Science and Technology, School of Bioresource & Bioscience, Chung-Ang University) ;
  • Park, Yoo-Jin (Department of Animal Science and Technology, School of Bioresource & Bioscience, Chung-Ang University) ;
  • Song, Won-Hee (Department of Animal Science and Technology, School of Bioresource & Bioscience, Chung-Ang University) ;
  • Mohamed, El-Sayed A. (Department of Animal Science and Technology, School of Bioresource & Bioscience, Chung-Ang University) ;
  • Pang, Myung-Geol (Department of Animal Science and Technology, School of Bioresource & Bioscience, Chung-Ang University)
  • 오신애 (중앙대학교 생명자원공학부 동물생명공학) ;
  • 박유진 (중앙대학교 생명자원공학부 동물생명공학) ;
  • 송원희 (중앙대학교 생명자원공학부 동물생명공학) ;
  • ;
  • 방명걸 (중앙대학교 생명자원공학부 동물생명공학)
  • Received : 2011.02.21
  • Accepted : 2011.02.28
  • Published : 2011.03.31
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Abstract

Endocrine disruptors bind to hormone receptors on sperm membrane, therefore spermatozoa are potentially a useful model for examining estrogenic activities of endocrine disruptors. The objective of this study was to compare the effects of two xenoestrogenic compounds [genistein (Gen) and 4-tert-octylphenol (OP)] to those of two steroids [estrogen ($E_2$) and progesterone ($P_4$)] on boar sperm % motility and motion kinematics of in vitro. Porcine spermatozoa were incubated with various concentrations ($0.001{\sim}100\;{\mu}M$) of each chemical for 15 or 30 min, and then assessed % motility and sperm motion kinematics using computer assisted sperm analyzer (CASA). Each chemical decreased sperm % motility, and OP decreased VSL and VAP compared with untreated control(p<0.05). $E_2$ stimulated the motion kinematic changes except VCL. Moreover, Gen had effects on VCL and VAP alterations after 30 min incubation. In summary, since all chemicals studied effectively altered sperm % motility and motion kinematics, it was concluded that porcine spermatozoa could be a useful model for in vitro screening of potential endocrine disruptors.

Keywords

References

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