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노니 과실에 함유된 항산화물의 추출 공정

Extraction Procedures for Free Radical Scavenging Activity from Noni Fruit (Morinda citrifolia)

  • 곽미경 (자혜식품(주) 연구개발부) ;
  • 최희선 (인제대학교 식품생명과학부) ;
  • ;
  • 홍정화 (인제대학교 식품생명과학부)
  • Gwak, Mi-Kyung (Jahye Food Co. Ltd.) ;
  • Choi, Hee-Sun (Biohealth Products Research Center, School of Food and Life Science, Inje University) ;
  • Manochai, Benya (Faculty of Agriculture, Department of Horticulture, Kasetsart University) ;
  • Hong, Jeong-Hwa (Biohealth Products Research Center, School of Food and Life Science, Inje University)
  • 투고 : 2011.01.21
  • 심사 : 2011.02.25
  • 발행 : 2011.02.28

초록

In this study, we investigated maximum yield of radical scavenging activity (RSA) using each of various extraction solvents and procedure from noni friut (Morinda citrifolia). Noni fruits were either sun-dried (FS) or hot-air dried (FO) at $60^{\circ}C$ after steam blanching. For optimum aqueous extraction, noni fruits should be sun dried and autoclave extraction time should not be over 30 min to produce extraction with high RSA with low cost. In case of 50% ethanol extraction, reflux extracts of FS and FO resulted in $IC_{50}$ of 1.92 mg/mL and 3.06 mg/mL at 8 hr. When $IC_{50}$ values were lower than 5 mg/mL, coefficient of correlation was 0.71 indicating that 71% of the phenolic antioxidants in noni fruits were accounted for the activity by scavenging free DPPH. However, coefficient of correlation significantly decreased to 0.63 over $IC_{50}$ values of 5 mg/mL. Autoclave extraction contained chlorogenic acid of $14.69 \;{\mu}g/mL$ and scopoletin of $3.86 \;{\mu}g/mL$. Reflux extraction showed all three compounds, chlorogenic acid ($26.19 \;{\mu}g/mL$), quercetin ($19.59 \;{\mu}g/mL$), and scopoletin ($17.4 \;{\mu}g/mL$). Therefore, the result of this study indicated that the potential antioxidant activities and functional values were obtained significantly with reflux extraction from noni fruit.

키워드

참고문헌

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피인용 문헌

  1. Study on the Bioactive Characteristics of Morinda citrifolia as a Cosmetic Raw Material vol.42, pp.2, 2016, https://doi.org/10.15230/SCSK.2016.42.2.183