Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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The Effect of LhGH on Hair Regeneration in C57BL/6CrN Mouse

LhGH가 마우스(C57BL/6CrN)의 모발 재성장에 미치는 영향

  • Kim, Yong-Ju (Department of Life Science, College of Natural Science, Hallym University) ;
  • Kim, Tae-Keun (Department of Life Science, College of Natural Science, Hallym University) ;
  • Min, Byoung-Hoon (Department of Parasitology, Korea University College of Medicine and Institute of Travel Medicine Korea University) ;
  • Kim, Soo-Jin (Department of Life Science, College of Natural Science, Hallym University)
  • 김용주 (한림대학교 자연과학대학 생명과학과) ;
  • 김태근 (한림대학교 자연과학대학 생명과학과) ;
  • 민병훈 (고려대학교 의과대학 기생충학교실 및 여행의학연구소) ;
  • 김수진 (한림대학교 자연과학대학 생명과학과)
  • Received : 2011.03.07
  • Accepted : 2011.03.24
  • Published : 2011.03.31
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Abstract

Hair is an appendage of skin which protects the body from outer physical and chemical stimuli. Hair is generated from the hair follicle lying on a sunken basal layer of epidermis. Hair cycling, which regenerates hair follicles throughout the life time of the organism. Numerous kinds of factors which exist at the hair follicle have been reported to regulate hair cycling, Human growth hormone secreted from pituitary gland, initially demonstrated to accelerate organ's growth, has been reported to play a role in the biology of organ size determination. We investigated the effect of 6-histidines residues tagged at amino-terminus of human growth hormone using light and electronmicroscopic methods. Human growth hormone encapsulated in nano-liposome (LhGH) was used to find how LhGH affects hair follicle cycling of mouse (C57BL6/CrN). Distilled water as a negative control, 3% Minoxidil as a positive control, and LhGH were applied to mouse for weeks. LhGH increased the number of exposed hairs per given areas ($1mm^2$). This result was also confirmed using a different breed of mice which show natural hair loss in an old age (about 17 months after birth). When LhGH was applied for 3 weeks after natural hair loss, natural hair loss on these mice was prevented, However, the control group mice on which LhGH was not applied showed further hair loss. This result indicates that LhGH may stimulate hair cycling of mouse. In clusion, it is cleat that the LhGH increased the number of hair on mice and help the depilated skin to grow new hair follicles again.

모발은 피부를 둘러싸고 있는 피부 부속기관이며, 외부로부터 들어오는 물리적, 화학적 자극에 대하여 보호하고, 미용 효과 등의 기능을 가지고 있다. 모발은 피부의 표피와 진피의 사이에서 발생하며, 모발성장주기를 가지고 있다. 모발성장주기는 모발이 성장하는 시기(anagen)와 성장을 끝내는 시기(catagen), 성장을 멈추고 휴식하는 시기(telogen)로 구분할 수 있다. 모발성장기 중, 성장기의 모낭 부위에는 많은 성장인자들이 분포하고 있다는 것이 이미 보고된 바 있다. 뇌하수체에서 분비되는 인간 성장 호르몬은 신진대사를 수행하는 신호를 전달하며, 기관의 성장을 촉진하는 역할을 수행한다. 성장호르몬은 혈류를 따라 온몸으로 이동되며, 여러 가지의 성장인자들을 활성하거나 억제하는 조절 작용을 한다는 연구결과가 보고된 바 있다. 본 연구에서는 인간성장호르몬을 유전자재조합기술을 이용하여 관찰이 용이하도록 6개의 히스티딘 아미노산을 첨가하였으며, 나노크기의 리포좀으로 캡슐화한 LhGH를 (주)리제론으로 부터 받아 사용하여 마우스(C57BL6/CrN)에서 LhGH에 의한 모발의 성장 관계를 알아보았다. 8주령의 마우스 3마리씩 증류수, Liposome, Minoxidil, His hGH, LhGH를 실험의 목적에 따라 20일, 6주 동안 도포하였고, 피부 속의 침투 경로는 형광면역반응법을 이용하여 형광현미경으로 관찰하였다. 또한, 광학현미경과 주사전자현미경을 이용하여 모발 수의 변화를 비교하여 보았다. 마우스 피부에 도포한 LhGH는 피부표피의 모공과 모발을 따라 피부 안으로 이동되는 것이 광학현미경으로 관찰되었다. 또한 LhGH는 성별에 관계없이 모발 수를 증가시켰으며, 피부 밖으로 도출된 모발 수를 증가시킴을 확인하였다. 자연적으로 탈모된 마우스에서도 발모를 증진시켰으며, 발모 후 3주 동안 새로운 탈모현상을 억제하였다. 이러한 결과로 LhGH는 마우스(C57BL/6CrN)에서 모발의 수를 증가시키며, 탈모된 피부에서 모발의 재성장을 촉진시키는 효과가 있다고 생각한다.

Keywords

References

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