Journal of Korean Academy of Nursing (대한간호학회지)

Korean Society of Nursing Science (한국간호과학회)
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The Effect of Treadmill Exercise on Ischemic Neuronal Injury in the Stroke Animal Model: Potentiation of Cerebral Vascular Integrity

중풍 동물 모델에서의 트레드밀 운동이 허혈성 신경손상에 미치는 효과: 뇌혈관 통합성 강화

  • Kang, Kyoung-Ah (Department of Nursing, Mokpo Catholic University) ;
  • Seong, Ho-Hyun (College of Nursing Science, Kyung Hee University) ;
  • Jin, Han-Byeol (College of Nursing Science, Kyung Hee University) ;
  • Park, Jong-Min (College of Nursing Science, Kyung Hee University) ;
  • Lee, Jong-Min (Department of Rehabilitation, College of Medicine, Konkuk University) ;
  • Jeon, Jae-Yong (Department of Rehabilitation Medicine, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Kim, Youn-Jung (College of Nursing Science, East West Nursing Institute, Kyung Hee University)
  • 강경아 (목포가톨릭 대학교 간호학과) ;
  • 성호현 (경희대학교 간호과학대학) ;
  • 진한별 (경희대학교 간호과학대학) ;
  • 박종민 (경희대학교 간호과학대학 대학원) ;
  • 이종민 (건국대학교 의과대학 재활의학과) ;
  • 전재용 (울산대학교 의과대학 서울아산병원 재활의학교실) ;
  • 김연정 (경희대학교 간호과학대학·동서간호학연구소)
  • Received : 2010.06.26
  • Accepted : 2011.04.15
  • Published : 2011.04.30
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Abstract

Purpose: This study was done to identify whether pre-conditioning exercise has neuroprotective effects against cerebral ischemia, through enhance brain microvascular integrity. Methods: Adult male Sprague-Dawley rats were randomly divided into four groups: 1) Normal (n=10); 2) Exercise (n=10); 3) Middle cerebral artery occlusion (MCAo), n=10); 4) Exercise+MCAo (n= 10). Both exercise groups ran on a treadmill at a speed of 15 m/min, 30 min/day for 4 weeks, then, MCAo was performed for 90 min. Brain infarction was measured by Nissl staining. Examination of the remaining neuronal cell after MCAo, and microvascular protein expression on the motor cortex, showed the expression of Neuronal Nuclei (NeuN), Vascular endothelial growth factor (VEGF) & laminin. Results: After 48 hr of MCAo, the infarct volume was significantly reduced in the Ex+MCAo group ($15.6{\pm}2.7%$) compared to the MCAo group ($44.9{\pm}3.8%$) (p<.05), and many neuronal cells were detected in the Ex+ MCAo group ($70.8{\pm}3.9%$) compared to the MCAo group ($43.4{\pm}5.1%$) (p<.05). The immunoreactivity of laminin, as a marker of microvessels and Vascular endothelial growth factor (VEGF) were intensively increased in the Ex+MCAo group compared to the MCAo group. Conclusion: These findings suggest that the neuroprotective effects of exercise pre-conditioning reduce ischemic brain injury through strengthening the microvascular integrity after cerebral ischemia.

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References

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