Journal of the Korean Wood Science and Technology

  • 제39권2호
  • /
  • Pages.187-195
  • /
  • 2011
  • /
  • 1017-0715(pISSN)
  • /
  • 2233-7180(eISSN)
한국목재공학회 (The Korean Society of Wood Science & Technology)
권호 표지
DOI QR코드

DOI QR Code

Effects of Dilute Acid Pretreatment on Enzyme Adsorption and Surface Morphology of Liriodendron tulipifera

  • Min, Byeong-Cheol (Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Koo, Bon-Wook (Department of Wood and Paper Science, College of Natural Resource, 2105 Biltmore Hall, North Carolina State University) ;
  • Gwak, Ki-Seob (Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Yeo, Hwan-Myeong (Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Choi, Joon-Weon (Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Choi, In-Gyu (Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University)
  • 투고 : 2011.02.28
  • 심사 : 2011.03.14
  • 발행 : 2011.03.25
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In this study, dilute acid pretreatment of $Liriodendron$ $tulipifera$ was performed for enzymatic hydrolysis. As the pretreatment temperature was increased, enzymatic hydrolysis and enzyme adsorption yield also increased. The highest enzymatic hydrolysis yield was 57% (g/g) and enzyme adsorption was 44% (g/g). Enzymatic hydrolysis yield was determined with weight loss of pretreated biomass by enzyme, and enzyme adsorption was a percentage of enzyme weight attaching on pretreated biomass compared with input enzyme weight. When $L.$ $tulipifera$ was pretreated with 1% sulfuric acid at $160^{\circ}C$ for 5 min., hemicellulose was significantly removed in pretreatment, but the lignin contents were constant. Other changes in surface morphology were detected on biomass pretreated at $160^{\circ}C$ by a field emission scanning electron microscope (FESEM). A large number of spherical shapes known as lignin droplets were observed over the entire biomass surface after pretreatment. Hemicellulose removal and morphological changes improved enzyme accessibility to cellulose by increasing cellulose exposure to enzyme. It is thus evidence that enzyme adsorption is a significant factor to understand pretreatment effectiveness.

키워드

참고문헌

  1. Galbe, M. and G. Zacchi. 2002. A review of the production of ethanol from softwood. Applied Microbiology and Biotechnology 59: 618-628. https://doi.org/10.1007/s00253-002-1058-9
  2. Sun, Y. and J. Cheng. 2002. Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresource Technology 83: 1-11. https://doi.org/10.1016/S0960-8524(01)00212-7
  3. Cosgrove, D. 2005. Growth of the plant cell wall. Nature Reviews Molecular Cell Biology 6: 850. https://doi.org/10.1038/nrm1746
  4. Himmel, M., M. Ruth, and C. Wyman. 1999. Cellulase for commodity products from cellulosic biomass. Current Opinion in Biotechnology 10: 358-364. https://doi.org/10.1016/S0958-1669(99)80065-2
  5. Wyman, C., B. Dale, R. Elander, M. Holtzapple, M. Ladisch, and Y. Lee. 2005. Coordinated development of leading biomass pretreatment technologies. Bioresource Technology 96: 1959 -1966. https://doi.org/10.1016/j.biortech.2005.01.010
  6. Mosier, N., C. Wyman, B. Dale, R. Elander, Y. Lee, M. Holtzapple, et al. 2005. Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresource Technology 96: 673-686. https://doi.org/10.1016/j.biortech.2004.06.025
  7. Hendriks, A. and G. Zeeman. 2009. Pretreatments to enhance the digestibility of lignocellulosic biomass. Bioresource Technology 100: 10-18. https://doi.org/10.1016/j.biortech.2008.05.027
  8. Lee, J. W. 2007. Enzymatic saccharification of lignocellulosic biomass using wood rot fungi and characterization of related enzyme: Seoul National University.
  9. Sharma, S., K. Kalra, and H. Grewal. 2002. Enzymatic saccharification of pretreated sunflower stalks. Biomass and Bioenergy 23: 237- 243. https://doi.org/10.1016/S0961-9534(02)00050-8
  10. Sheehan, J. and M. Himmel. 1999. Enzymes, energy, and the environment: a strategic perspective on the US Department of Energy's research and development activities for bioethanol. Biotechnology Progress 15.
  11. Jeoh, T., C. Ishizawa, M. Davis, M. Himmel, W. Adney, and D. Johnson. 2007. Cellulase digestibility of pretreated biomass is limited by cellulose accessibility. Biotechnology and Bioengineering 98.
  12. Desvaux, M., E. Guedon, and H. Petitdemange. 2000. Cellulose Catabolism by Clostridium cellulolyticum Growing in Batch Culture on Defined Medium. Applied and Environmental Microbiology 66: 2461-2470. https://doi.org/10.1128/AEM.66.6.2461-2470.2000
  13. Kennedy, M., M. Thakur, D. Wang, and G. Stephanopoulos. 1992. Techniques for the estimation of cell concentration in the presence of suspended solids. Biotechnology Progress 8: 375 -381. https://doi.org/10.1021/bp00017a001
  14. Weimer, P., Y. Shi, and C. Odt. 1991. A segmented gas/liquid delivery system for continuous culture of microorganisms on insoluble substrates and its use for growth of Ruminococcus flavefaciens on cellulose. Applied Microbiology and Biotechnology 36: 178-183. https://doi.org/10.1007/BF00164416
  15. Findlay, R., G. King, and L. Watling. 1989. Efficacy of Phospholipid Analysis in Determining Microbial Biomass in Sediments. Applied and Environmental Microbiology 55: 2888-2893.
  16. Hashimoto, S., M. Fujita, and R. A. Baccay. 1982. Biomass Determination in the Anaerobic Digestion of Night Soil: Kinetic Studies on the Anaerobic Digestion of Night Soil (I). Journal of fermentation technology 60: 51-54.
  17. Kumar, R. and C. Wyman. 2008. An improved method to directly estimate cellulase adsorption on biomass solids. Enzyme and Microbial Technology 42: 426-433. https://doi.org/10.1016/j.enzmictec.2007.12.005
  18. Himmel, M., T. Vinzant, S. Bower, and J. Jecura. 2005. BSCL Use Plan : Solving biomass recalcitrance. In: NREL; 2005.
  19. Himmel, M., S. Ding, D. Johnson, W. Adney, M. Nimlos, J. Brady, et al. 2007. Biomass recalcitrance: engineering plants and enzymes for biofuels production. Science 315: 804. https://doi.org/10.1126/science.1137016
  20. TAPPI. 1991. Tappi Test Methods In: Tappi Press, Atlanta, GA.
  21. NREL. 2005. Biomass Analysis Technology Team Laboratory Analytical Procedure. US Department of Energy, National Renewable Energy Laboratory, Golden, CO.
  22. Baklanov, M. R., K. P. Mogilnikov, V. G. Polovinkin, and F. N. Dultsev. 2000. Determination of pore size distribution in thin films by ellipsometric porosimetry. Journal of Vacuum Science & Technology B: Microelectronics and Nanometer Structures 18: 1385. https://doi.org/10.1116/1.591390
  23. Lynd, L., P. Weimer, W. van Zyl, and I. Pretorius. 2002. Microbial Cellulose Utilization: Fundamentals and Biotechnology. Microbiology and Molecular Biology Reviews 66: 506. https://doi.org/10.1128/MMBR.66.3.506-577.2002
  24. Knappert, D., H. Grethlein, and A. Converse. 1981. Partial acid hydrolysis of poplar wood as a pretreatment for enzymatic hydrolysis[Populus, Trichoderma reesei C30, biomass fuels]. In: Wiley New York (USA).
  25. Nguyen, Q., M. Tucker, F. Keller, and F. Eddy. 2000. Two-stage dilute-acid pretreatment of softwoods. Applied Biochemistry and Biotechnology 84: 561-576. https://doi.org/10.1385/ABAB:84-86:1-9:561
  26. Cowling, E. and T. Kirk. 1976. Properties of cellulose and lignocellulosic materials as substrates for enzymatic conversion processes.
  27. Grethlein, H. 1985. The effect of pore size distribution on the rate of enzymatic hydrolysis of cellulosic substrates. Nature Biotechnology 3: 155-160. https://doi.org/10.1038/nbt0285-155
  28. Donohoe, B., S. Decker, M. Tucker, M. Himmel, and T. Vinzant. 2008. Visualizing lignin coalescence and migration through maize cell walls following thermochemical pretreatment. Biotechnology and Bioengineering 101.