Journal of Plant Biotechnology

  • 제38권1호
  • /
  • Pages.94-104
  • /
  • 2011
  • /
  • 1229-2818(pISSN)
  • /
  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

A transcription factor "OsNAC075" is essential for salt resistance in rice (Oryza sativa L.)

  • Jung, Yu-Jin (Institute of Genetic Engineering, Hankyong National University) ;
  • Lee, Myung-Chul (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Kang, Kwon-Kyoo (Institute of Genetic Engineering, Hankyong National University)
  • 투고 : 2011.03.16
  • 심사 : 2011.03.21
  • 발행 : 2011.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Salt stress is a major environmental factor influencing plant growth and development. To identify salt tolerance determinants, we systematically screened salt sensitive rice mutants by use of the Activator/Dissociation (Ac/Ds) transposon tagging system. In this study, we focused on the salt sensitive mutant line, designated SSM-1. A gene encoding a NAC transcription factor homologue was disrupted by the insertion of a Ds transposon into SSM-1 line. The OsNAC075 gene (EU541472) has 7 exons and encodes a protein (486-aa) containing the NAC domain in its N-terminal region. Sequence comparison showed that the OsNAC075 protein had a strikingly conserved region at the N-terminus, which is considered as the characteristic of the NAC protein family. OsNAC075 protein was orthologous to Arabidopsis thaliana ANAC075. Phylogenetic analysis confirmed OsNAC075 belonged to the OsNAC3 subfamily, which plays an important role in response to stress stimuli. RT-PCR analysis showed that the expression of OsNAC075 gene was rapidly and strongly induced by stresses such as NaCl, ABA and low temperature ($4^{\circ}C$). Our data suggest that OsNAC075 holds promising utility in improving salt tolerance in rice.

키워드

참고문헌

  1. Aida M, Ishida T, Fukaki H, Fujisawa H, Tasaka M (1997) Genes involved in organ separation in Arabidopsis: an analysis of the cup-shaped cotyledon mutant. Plant Cell 9:841-857 https://doi.org/10.1105/tpc.9.6.841
  2. Bray EA (2004) Genes commonly regulated by water-deficit stress in Arabidopsis thaliana. Journal of Experimental Botany 55:2331-2341 https://doi.org/10.1093/jxb/erh270
  3. Che P, Gingerich DJ, Lall S, Howell SH (2002) Global and Hormone-Induced Gene Expression Changes during Shoot Development in Arabidopsis. Plant Cell 14:2771-2785 https://doi.org/10.1105/tpc.006668
  4. Chin HG, Choe MS, Lee SH, Park SH, Koo JC, Kim NY, Lee JJ, Oh BG, Yi GH, Kim SC, Choi HC, Cho MJ, Han CD (1999) Molecular analysis of rice plants harboring an Ac/Ds transposable element-mediated gene trapping system. Plant J. 19:615-623 https://doi.org/10.1046/j.1365-313X.1999.00561.x
  5. Collinge M, Boller T (2001) Differential induction of two potato genes, Stprx2 and StNAC, in response to infection by Phyto phthora infestans and to wounding. Plant Mol Biol 46:521-529 https://doi.org/10.1023/A:1010639225091
  6. Duval M, Hsieh TF, Kim SY, Thomas TL (2002) Molecular characterization of AtNAM: a member of the Arabidopsis NAC domain superfamily. Plant Mol Biol 50:237-248 https://doi.org/10.1023/A:1016028530943
  7. Fowler S, Thomashow MF (2002) Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. Plant cell 14:1675-1690 https://doi.org/10.1105/tpc.003483
  8. Fujita M, Fujita Y, Maruyama K, Seki M, Hiratsu K, Ohme-Takagi M (2004) A dehydration-induced NAC protein, RD26, is involved in a novel ABA-dependent stresssignaling pathway. Plant J 39:863-876 https://doi.org/10.1111/j.1365-313X.2004.02171.x
  9. Greve K, La Cour T, Jensen MK, Poulsen FM, Skriver K (2003) Interactions between plant RING-H2 and plant-specific NAC (NAM/ATF1/2/CUC2) proteins: RING-H2 molecular specificity and cellular localization. Biochem J 371:97-108 https://doi.org/10.1042/BJ20021123
  10. Han CD (2002) Rice functional genomics by transposon mutagenesis. Asia Pacific Biotech News 6:930-935 https://doi.org/10.1142/S0219030302001933
  11. Hegedus D, Yu M, Baldwin D, Gruber M, Sharpe A, Parkin I (2003) Molecular characterization of Brassicanapus NAC domain transcriptional activators induced in response to biotic and abiotic stress. Plant Mol Biol 53:383-397 https://doi.org/10.1023/B:PLAN.0000006944.61384.11
  12. John I, Hackett R, Cooper W, Drake R, Farrell A, Grierson D (1997) Cloning and characterization of tomato leaf senescence-related cDNAs. Plant Mol Biol 33:641-651 https://doi.org/10.1023/A:1005746831643
  13. Kikuchi K, Ueguchi-Tanaka M, Yoshida KT, Nagato Y, Matsusoka M, Hirano HY (2000) Molecular analysis of the NAC gene family in rice. Mol Gen Genet 262:1047-1051 https://doi.org/10.1007/PL00008647
  14. Kim CM, Je BI, Piao HL, Park SJ, Kim MJ, Park SH, Park JY, Park SH, Lee EK, Chon NS, Won YJ, Lee GH, Nam MH, Yun DW, Lee MC, Cha YS, Lee KH, Eun MY, Han CD (2002) Reprogramming of the activity of the activator/dissociation transposon family during plant regeneration in rice. Mol Cells 14:231-237
  15. Kim CM, Piao HL, Park SJ, Chon NS, Je BI, Sun BY, Park SH, Park JY, Lee EJ, Kim MJ, Chung WS, Lee KH, Lee YS, Lee JJ, Won YJ, Lee GH, Nam MH, Cha YS, Yun DW, Eun MY, Han CD (2004) Rapid, large-scale generation of Ds transposant lines and analysis of the Ds insertion sites in ride. Plant J 39:252-263 https://doi.org/10.1111/j.1365-313X.2004.02116.x
  16. Liu YG, Whittier RF (1995) Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics 25:674-681 https://doi.org/10.1016/0888-7543(95)80010-J
  17. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissues. Physiol Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  18. Ooka H, Satoh K, Doi K, Nagata T, Otomo Y, Murakami K (2003) Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana. DNA Res 10: 239-247 https://doi.org/10.1093/dnares/10.6.239
  19. Quesada V, Ponce MR, Micol JL (2000) Genetic analysis of salt-tolerant mutants in Arabidopsis thaliana. Genetics 154:421-436
  20. Rabbani MA, Maruyama K, Abe H, Khan MA, Katsura K, Ito Y (2003) Monitoring expression profiles of rice genes under cold, drought, and high-salinity stresses and abscisic acid application using cDNA microarray and RNA gel-blot analyses. Plant Physiol 133:1755-1767 https://doi.org/10.1104/pp.103.025742
  21. Ren T, Qu F, Morris TJ (2000) HRT gene function requires interaction between a NAC protein and viral capsid protein to confer resistance to turnip crinkle virus. Plant Cell 12:1917-1926 https://doi.org/10.1105/tpc.12.10.1917
  22. Ruiz-Medrano R, Xoconostle-Cazares B, Lucas WJ (1999) Phloem long-distance transport of CmNACP mRNA: Implications for supracellular regulation in plants. Development 126:4405-4419
  23. Sablowski RW, Meyerowitz EM (1998) A homolog of NO APICAL MERISTEM is an immediate target of the floral homeotic genes APETALA3/PISTILLATA. Cell 92:93-103 https://doi.org/10.1016/S0092-8674(00)80902-2
  24. Saleki R, Young PG, Lefebvre DD (1993) Mutants of Arabidopsis thaliana capable of germination under saline conditions. Plant Physiology 101:839-845 https://doi.org/10.1104/pp.101.3.839
  25. Sasaki T, Matsumoto T, Yamamoto K, Sakata K, Baba T, Katayose Y, Wu J, Niimura Y, Cheng Z, Nagamura Y (2002) The genome sequence and structure of rice chromosome 1. Nature 420:312-316 https://doi.org/10.1038/nature01184
  26. Seki M, Narusaka M, Ishida J (2002b) Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. The Plant Journal 31:279-292 https://doi.org/10.1046/j.1365-313X.2002.01359.x
  27. Selth LA, Dogra SC, Rasheed MS, Healy H, Randles JW, Rezaian MA (2005) A NAC domain protein interacts with Tomato leaf curl virus replication accessory protein and enhances viral replication. Plant Cell 17:311-325 https://doi.org/10.1105/tpc.104.027235
  28. Shi R, Pryor JD (2000) Temperature dependence of membrane sealing following transection in mammalian spinal cord axons. Neuroscince 98:157-166 https://doi.org/10.1016/S0306-4522(00)00096-8
  29. Shinozaki K, Yamaguchi-Shinozaki K, Seki M (2003) Regulatory network of gene expression in the drought and cold stress responses. Current Opinion in Plant Biology 6:410-417 https://doi.org/10.1016/S1369-5266(03)00092-X
  30. Souer E, van Houwelingen A, Kloos D, Mol J, Koes R (1996) The no apical meristem gene of Petunia is required for pattern formation in embryos and flowers and is expressed at meristem and primordia boundaries. Cell 85:159-170 https://doi.org/10.1016/S0092-8674(00)81093-4
  31. Tran LS, Nakashima K, Sakuma Y, Simpson SD, Fujita Y, Maruyama K (2004) Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter. Plant Cell 16: 2481-2498 https://doi.org/10.1105/tpc.104.022699
  32. Tsugane K, Kobayashi K, Niwa Y, Ohba Y, Wada K, Kobayashi H (1999) A recessive Arabidopsis mutant that grows photoautotrophically under salt stress shows enhanced active oxygen detoxification. Plant Cell 11:1195-1206 https://doi.org/10.1105/tpc.11.7.1195
  33. Tsugeki R, Kochieva EZ, Fedoroff NV (1996) A transposon insertion in the Arabidopsis SSR16 gene causes an embryodefective lethal mutation. Plant J 10:479-489 https://doi.org/10.1046/j.1365-313X.1996.10030479.x
  34. Weir I, Lu J, Cook H, Causier B, Schwarz-Sommer Z, Davies B (2004) CUPULIFORMIS establishes lateral organ boundaries in Antirrhinum. Development 131:915-922 https://doi.org/10.1242/dev.00993
  35. Werner JE, Finkelstein RR (1995) Arabidopsis mutants with reduced response to NaCl and osmotic stress. Physiol Plant 93:659-666 https://doi.org/10.1111/j.1399-3054.1995.tb05114.x
  36. Wu SJ, Ding L, Zhu JK (1996) SOS1, a genetic locus essential for salt tolerance and potassium acquisition. Plant Cell 8:617-627 https://doi.org/10.1105/tpc.8.4.617
  37. Xie Q, Frugis G, Colgan D, Chua NH (2000) Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development. Genes Dev 14:3024-3036 https://doi.org/10.1101/gad.852200
  38. Xiong L, Schumaker KS, Zhu JK (2002) Cell signaling during cold, drought, and salt stress. Plant Cell 14:165-183 https://doi.org/10.1105/tpc.010278
  39. Yamaguchi-Shinozaki K, Shinozaki K (2006) Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annu Rev Plant Biol 57: 781-803 https://doi.org/10.1146/annurev.arplant.57.032905.105444
  40. Zhu JK (2000) Genetic Analysis of Plant Salt Tolerance Using Arabidopsis. Plant Physiology 124:941-948 https://doi.org/10.1104/pp.124.3.941

피인용 문헌

  1. Development of high tryptophan GM rice and its transcriptome analysis vol.42, pp.3, 2015, https://doi.org/10.5010/JPB.2015.42.3.186
  2. Apple russeting as seen through the RNA-seq lens: strong alterations in the exocarp cell wall vol.88, pp.1-2, 2015, https://doi.org/10.1007/s11103-015-0303-4
  3. Quantitative trait loci analysis of root traits under phosphorus deficiency at the seedling stage in wheat vol.61, pp.3, 2018, https://doi.org/10.1139/gen-2017-0159