한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제28권1호
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  • Pages.57-62
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  • 2011
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

탈세포화 기법을 이용한 돼지 바이오 스캐폴드: 환자 맞춤형 장기재생을 위한 역분화 줄기세포 동물모델

Decellularized Bioscaffold of Pig Organs: A Tool for Patient-specific Organogenesis Using Induced Pluripotent Stem Cells

  • 박경미 (강원대학교 줄기세포 연구소) ;
  • 곽호현 (강원대학교 줄기세포 연구소) ;
  • 남현숙 (강원대학교 줄기세포 연구소) ;
  • 박인철 (강원대학교 줄기세포 연구소) ;
  • 전용환 (강원대학교 줄기세포 연구소) ;
  • 박성민 (강원대학교 줄기세포 연구소) ;
  • 이승태 (강원대학교 줄기세포 연구소) ;
  • 우제석 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 우흥명 (강원대학교 줄기세포 연구소)
  • 심사 : 2011.02.16
  • 발행 : 2011.02.28
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초록

The shortage of transplantable kidneys has many efforts to regenerate bioartificial kidneys using transgenic animals and diverse kinds of scaffolds which are important tools for cell seeding. However, there are many limitations for clinical applications so far. Recently, decellularized bioscaffolds using animal organs come into spotlight because of its many superior advantages. In current study, we produced decellularized kidney bioscaffolds of pig which is an attractive animal as a clinical model for human. We decellularized pig kidneys with 1% SDS detergent solution using peristaltic pump systems for 12h. After decellularization process, the kidney bioscaffolds preserved intact 3D morphology including glomerular structure and almost DNA from pig was entirely removed. In addition, this process could preserve micro vascular network which is necessary for cell survival. Although, additional studies for recellularization and transplantation should be required, the decellular vascularized kidney bioscaffolds might have many potentials for kidney regeneration.

키워드

참고문헌

  1. 남현숙, 엄지용, 윤병일, 우흥명. 개의 신장 동종이식 후 발생된 급성 거부반응. 한국임상수의학회지 2005; 22: 439-449.
  2. Are C, Kutka M. Effect of laparoscopic antireflux surgery upon renal blood flow. The American Journal of Surgery 2002; 183: 419-423. https://doi.org/10.1016/S0002-9610(02)00824-3
  3. Badylak SF, Lantz GC, Coffey A, Geddes LA. Small intestinal submucosa as a large diameter vascular graft in the dog. J Surg Res 1989; 47: 74-80. https://doi.org/10.1016/0022-4804(89)90050-4
  4. Badylak SF. Xenogeneic extracellular matrix as a scaffold for tissue reconstruction. Transpl Immunol 2004; 12: 367-77. https://doi.org/10.1016/j.trim.2003.12.016
  5. Esteban MA, Jianyong X, Generation of induced pluripotent stem cell lines from Tibetan miniature pig. The journal of biological chemistry 2009; 284, 17634-17640. https://doi.org/10.1074/jbc.M109.008938
  6. Karina H, Nakayama BS. Decellularized Rhesus monkey kidney as a three - dimensional scaffold for renal tissue engineering. Tissue Engineering 2009; 16: 2207-2216.
  7. Klymiuk N, Aigner B. Genetic modification of pigs as organ donors for xenotransplantation. Molecular Reproduction & Development 2010; 77: 209-221.
  8. Krejci NC, Cuono CB, Langdon RC, McGuire J. In vitro reconstitution of skin fibroblasts facilitate keratinocyte growth and differentiation on acellular reticular dermis. J Invest Dermatol 1991; 97: 843-848. https://doi.org/10.1111/1523-1747.ep12491522
  9. Ott HC, Clippinger B. Regeneration and orthotopic transplantation of a bioartificial lung. Nature medicine 2010; 16: 927-33. https://doi.org/10.1038/nm.2193
  10. Ott HC, Matthiesen TS. Perfusion - decellularized matrix: using nature's platform to engineer a bioartificial heart. Nature medicine 2008; 14: 2130-2139.
  11. Petersen TH, Calle EA. Tissue-engineered lungs for in vivo implantation. Science 2010; 329: 538-541. https://doi.org/10.1126/science.1189345
  12. Ross EA, Williams MJ. Embryonic stem cells proliferate and differentiate when seeded into kidney scaffolds. J Am Soc Nephrol 2009; 20: 2338-2347. https://doi.org/10.1681/ASN.2008111196
  13. Seebacher G, Grasl C. Biomechanical properties of decellularized porcine pulmonary valve conduits. Artif Organs 2008; 32: 28-35.
  14. Uygun BE, Soto-Gutierrez A. Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix. Nature medicine 2010; 16: 814-820. https://doi.org/10.1038/nm.2170
  15. Van PD, Nahmias Y. Human immune reactivity against liver sinusoidal endothelial cells from GalT$\alpha$(1,3)GalT-deficient pigs. Cell Transplant. 2010; 19: 783-789. https://doi.org/10.3727/096368910X508898
  16. Wainwright JM, Czajka CA. Preparation of cardiac extracellular matrix from an intact porcine heart. Tissue Engineering 2010; 16: 527-532.
  17. Walles T, Herden T, Haverich A, Mertsching H. Influence of scaffold thickness and scaffold composition on bioartificial graft survival. Biomaterials 2003; 24: 1233-1239. https://doi.org/10.1016/S0142-9612(02)00490-8
  18. Xu HM, Wang ZJ. Application of acellular dermal matrix for intestinal elongation in animal models. World J Gastroentero 2010; 16: 2023-2027. https://doi.org/10.3748/wjg.v16.i16.2023
  19. Yoo JJ, Meng J, Oberpenning F, Atala A. Bladder augmentation using allogenic bladder submucosa seeded with cells. Urology 1998; 51: 221-225. https://doi.org/10.1016/S0090-4295(97)00644-4
  20. Zhong R. Gal knockout and beyond. Am J Transplant 2007; 7: 5-11.
  21. Zhou J, Fritze O. Impact of heart valve decellularization on 3-D ultrastructure, immunogenicity and thrombogenicity. Biomaterials 2010; 31: 2549-2554. https://doi.org/10.1016/j.biomaterials.2009.11.088
  22. Zimmermann WH, Robert C. Cardiac tissue engineering: Implications for pediatric heart surgery. Pediatr Cardiol 2009; 30: 716-723. https://doi.org/10.1007/s00246-009-9405-6