Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Genotypic Characterization of Cherry Witches' Broom Pathogen Taphrina wiesneri Strains

벚나무 빗자루병균 Taphrina wiesneri의 유전적 특성

  • Seo, Sang-Tae (Division of Forest Insect Pests & Diseases, Korea Forest Research Institute) ;
  • Jeong, Su-Jee (Division of Forest Insect Pests & Diseases, Korea Forest Research Institute) ;
  • Lee, Seung-Kyu (Division of Forest Insect Pests & Diseases, Korea Forest Research Institute) ;
  • Kim, Kyung-Hee (Division of Forest Insect Pests & Diseases, Korea Forest Research Institute)
  • 서상태 (국립산림과학원 산림병해충연구과) ;
  • 정수지 (국립산림과학원 산림병해충연구과) ;
  • 이승규 (국립산림과학원 산림병해충연구과) ;
  • 김경희 (국립산림과학원 산림병해충연구과)
  • Received : 2010.11.26
  • Accepted : 2011.02.23
  • Published : 2011.04.30
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Abstract

The ascomycetous fungus Taphrina wiesneri, the pathogen of cherry witches' broom, is highly pathogenic to Prunus yedoensis, the most widely planted cherry trees in Korea as park and roadside trees. A collection of 13 strains of the pathogen in Korea and Japan was characterized by 18S rDNA gene sequence and restriction fragment length polymorphism (RFLP) analysis. In cluster analysis based on 18S rDNA gene sequence the strains were divided into 2 clusters. In RFLP analysis of the rDNA-IGS region using HhaI, the strains were separated into four patterns, B, C, D and G, of which pattern G was new.

자낭균인 Taphrina wiesneri는 한국의 공원과 가로수에 주로 식재되는 왕벚나무에 빗자루병을 일으키는 병원균이다. 한국과 일본에서 분리한 13개의 병원균에 대해 18S rDNA 염기서열 분석을 통한 계통학적 분석과 rDNA-IGS 영역에 대한 RFLP 분석을 실시하였다. 18S rDNA 염기서열 분석을 통한 계통도 분석결과 병원균은 2그룹으로 분류되었다. Hha I 제한효소를 이용한 rDNA-IGS 영역에 대한 RFLP 분석결과 B, C, D, G 4개의 패턴으로 나타났으며, 그중 G 패턴은 새로운 패턴이었다.

Keywords

References

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