The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Diversity of Arbuscular Mycorrhizal Fungi of Woody Plants in Mt. Munan

문안산의 목본식물과 공생하는 수지상균근균의 다양성

  • Park, Sang-Hee (Department of Biology Education, Korea National University of Education) ;
  • Eo, Ju-Kyeong (Department of Forest Science, Seoul National University) ;
  • Ka, Kang-Hyeon (Department of Forest Resources Utilization, Korea Forest Research Institute) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 박상희 (한국교원대학교 생물교육과) ;
  • 어주경 (서울대학교 산림과학부 산림환경) ;
  • 가강현 (국립산림과학원 녹색자원이용부) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2010.12.09
  • Accepted : 2011.03.08
  • Published : 2011.04.01
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Abstract

This study was conducted to reveal the diversity of arbuscular mycorrhizal fungi (AMF) in Mt. Munan, based on the morphological and molecular characters. Soil and root samples were collected from the rhizosphere of host plants including Lindera obtusiloba, Stephanandra incisa, Styrax obassis and Symplocos sawafutagi and AMF were trap-cultured with Sorghum bicolor as a host plant in a greenhouse. The spores were extracted from the cultured soils and five species were identified using morphological and molecular characteristics; Acaulospora longula, A. mellea, Ambispora leptoticha, Gigaspora margarita and Paraglomus occultum. The distribution of AMF showed different trends according to host plants. The dominant AMF species were A. longula in L. obtusiloba, A. leptoticha in S. incisa, S. obassis and S. sawafutagi.

본 연구에서는 수지상균근균(AMF)의 형태적 및 분자 생물학적 특징을 바탕으로 문안산에 분포하는 수지상균근균의 종 다양성을 분석하였다. AMF 시료는 생강나무(Lindera obtusiloba), 국수나무(Stephanandra incisa), 쪽동백(Styrax obassis), 노린재나무(Symplocos sawafutagi)의 뿌리와 뿌리 부근의 토양에서 채취하였으며, 수수(Sorghum bicolor)를 숙주식물로 사용하여 포자를 배양하였다. 추출된 포자들의 형태 형질과 염기서열을 분석한 결과 Acaulospora longula, A. mellea, Ambispora leptoticha, Gigaspora margarita, Paraglomus occultum 등 4속 5종이 동정되었으며, 숙주식물에 따른 수지상균근균의 분포는 수종에 따라서 다른 경향을 보였다. 생강나무에서는 A. longula가, 국수나무, 쪽동백나무, 노린재나무에서는 A. leptoticha가 우점종이었다.

Keywords

References

  1. 강석범, 문두길, 정종배. 2003. 화산회토양에서 인산 시용 수준별 탱자 유묘의 공생 균근 형성과 생육 및 무기양분 흡수. 한국토양비료학회 36:311-322.
  2. 구창덕, 김태훈, 이창근, 이원규, 강창호, 이병천, 이승규. 1992. 우리나라의 산림토양에 분포하는 포자낭과를 형성하는 아버스큘균근균, Glomus 속. 한국균학회지 20(1):29-36.
  3. 손보균, 허상만, 김길용. 1994. 딸기 묘 초기생육에 미치는 VA 균근균의 접종효과. 한국토양비료학회지 27(1):54-63.
  4. 이경준, 구창덕. 1983. 한국의 목본식물의 외생 및 내생균근에 관한 분류학적 분포 조사. 한국임학회지 59:37-45.
  5. 박향미, 강항원, 감위금, 박경배, 이상선, 송승달. 1999. Arbuscular mycorrhiza의 접종방법 및 인산시용량이 고추(Capsicum annum L.)의 초기생장에 미치는 영향. 한국토양비료학회 32:68-75.
  6. Bever, J., Morton, J., Antonovics, J. and Schultz, P. 1996. Hostdependent sporulation and species diversity of arbuscular mycorrhizal fungi in a mown grassland. J. Ecol. 84:71-82. https://doi.org/10.2307/2261701
  7. Daniels, B. and Skipper, H. 1982. Methods for the recovery and quantitative estimation of propagules from soil. In: Shenck N. C. ed. Methods and principles of mycorrhizal research. Am. Pathol. Soc., St. Paul, Minnesota, pp. 29-35.
  8. Eom, A. H, Hartnett, D. and Wilson, G. 2000. Host plant species effects on arbuscular mycorrhizal fungal communities in tallgrass prairie. Oecologia 122:435-444. https://doi.org/10.1007/s004420050050
  9. Eom, A. H. and Lee. S. S. 1989. Endomycorrhizal fungi identified on the soils in forest and coast areas. Kor. J. Mycol. 17:14-20.
  10. Finlay, R. 2008. Ecological aspects of mycorrhizal symbiosis: with special emphasis on the functional diversity of interactions involving the extraradical mycelium. J. Exp. Bot. 59:1115. https://doi.org/10.1093/jxb/ern059
  11. Gamper, H., Walker, C. and Schüβler, A. 2009. Diversispora celata sp. nov: molecular ecology and phylotaxonomy of an inconspicuous arbuscular mycorrhizal fungus. New Phytol. 182:495-506. https://doi.org/10.1111/j.1469-8137.2008.02750.x
  12. Giovannetti, M. and Gianinazzi-Pearson, V. 1994. Biodiversity in arbuscular mycorrhizal fungi. Mycol. Res. 98:705-715. https://doi.org/10.1016/S0953-7562(09)81041-1
  13. Hetrick, B. and Bloom, J. 1986. The influence of host plant on production and colonization ability of vesicular-arbuscular mycorrhizal spores. Mycologia 78:32-36. https://doi.org/10.2307/3793373
  14. Koske, R. and Gemma, J. 1989. A modified procedure for staining roots to detect VA mycorrhizas. Mycol. Res. 92:486-488. https://doi.org/10.1016/S0953-7562(89)80195-9
  15. Lee, J. K. 2003. Molecular biological identification of arbuscular mycorrhizal fungi collected from plant roots. MS Dissertation, Korea National University of Education, Cheongweon, Korea.
  16. Millner, P. and Kitt, D. 1992. The Beltsville method for soilless production of vesicular-arbuscular mycorrhizal fungi. Mycorrhiza 2:9-15. https://doi.org/10.1007/BF00206278
  17. Morton, J. and Redecker, D. 2001. Two new families of Glomales, Archaeosporaceae and Paraglomaceae, with two new genera Archaeospora and Paraglomus, based on concordant molecular and morphological characters. Mycologia 93:181-195. https://doi.org/10.2307/3761615
  18. Pik, M., Moora, M., Zobel, M., Saks, U., Wheatley, R., Wright, F. and Daniell, T. 2008. High diversity of arbuscular mycorrhizal fungi in a boreal herb rich coniferous forest. New Phytol. 179:867-876. https://doi.org/10.1111/j.1469-8137.2008.02515.x
  19. Redecker, D. and Raab, P. 2006. Phylogeny of the Glomeromycota (arbuscular mycorrhizal fungi): recent developments and new gene markers. Mycologia 98:885. https://doi.org/10.3852/mycologia.98.6.885
  20. Sanders, I. and Fitter, A. 1992. Evidence for differential responses between host-fungus combinations of vesicular-arbuscular mycorrhizas from a grassland. Mycol. Res. 96:415-419. https://doi.org/10.1016/S0953-7562(09)81084-8
  21. Schenck, N. and Perez, Y. 1990. Manual for the identification of VA mycorrhizal fungi. 3rd Ed. Synergistic, University of Florida, Gainesville.
  22. Smith, S. E. and Read, D. J. 2008. Mycorrhizal symbiosis. Academic Press.
  23. Tamura, K., J. Dudley, J., Nei, M. and Kumar, S. 2007. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24: 1596. https://doi.org/10.1093/molbev/msm092
  24. Van Tuinen, D., Jacquot, E., Zhao, B., Gollotte, A. and Gianinazzi-Pearson, V. 1998. Characterization of root colonization profiles by a microcosm community of arbuscular mycorrhizal fungi using 25S rDNA-targeted nested PCR. Mol. Ecol. 7:879-887. https://doi.org/10.1046/j.1365-294x.1998.00410.x
  25. Vandenkoornhuyse, P., Mahe, S., Ineson, P., Staddon, P., Ostle, N., Cliquet, J., Francez, A., Fitter, A. and Young, J. 2007. Active rootinhabiting microbes identified by rapid incorporation of plantderived carbon into RNA. Proc. Nat. Ac. Sci. 104:16970. https://doi.org/10.1073/pnas.0705902104
  26. Walker, C., Vestberg, M., Demircik, F., Stockinger, H., Saito, M., Sawaki, H., Nishmura, I. and SchuBler, A. 2007. Molecular phylogeny and new taxa in the Archaeosporales (Glomeromycota): Ambispora fennica gen. sp. nov., Ambisporaceae fam. nov., and emendation of Archaeospora and Archaeosporaceae. Mycol. Res. 111:137-153. https://doi.org/10.1016/j.mycres.2006.11.008
  27. Westover, K., Kennedy, A. and Kelley, S. 1997. Patterns of rhizosphere microbial community structure associated with co-occurring plant species. J. Ecol. 85:863-873. https://doi.org/10.2307/2960607

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