Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Repellent Activity of the Extracts of Acorus Gramineus against Mosquito (Culex pipinens pallens)

석창포 추출물의 모기에 대한 기피활성

  • Lee, Seung-Ho (Institute of Environmentally-friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Jung, Jin-Kwan (Youngil High School) ;
  • Park, Sang-Hyun (Youngil High School) ;
  • Kim, Mun-Sik (Youngil High School) ;
  • Han, Yeon-Soo (Institute of Environmentally-friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Seo, Jung-Mi (Gwangju Advanced Institute of Health and Environment) ;
  • Kim, In-Seon (Institute of Environmentally-friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University)
  • 이승호 (전남대학교 농업생명과학대학 친환경농업연구소) ;
  • 정진관 (영일고등학교) ;
  • 박상현 (영일고등학교) ;
  • 김문식 (영일고등학교) ;
  • 한연수 (전남대학교 농업생명과학대학 친환경농업연구소) ;
  • 서정미 (광주광역시 보건환경연구원) ;
  • 김인선 (전남대학교 농업생명과학대학 친환경농업연구소)
  • Received : 2011.02.08
  • Accepted : 2011.03.16
  • Published : 2011.03.31
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Abstract

BACKGROUND: Acorus gramineus is of medicinal plants that exhibit variable biological activities for human health and against insect pests. The extracts of A. gramineus was examined in an attempt to develop a natural repellent against human disease-mediating Culex pipiens. METHODS AND RESULTS: The roots of A. gramineus dried under dark conditions were homogenized and extracted with ethanol. The extracts were subjected to repellent activity assays against C. pipiens in a hand-made acrylamide box with three accessible rooms. Significantly low number of mosquitos was found in the room previously fumigated with the extracts at 50 mg/L on the filter paper, exhibiting less than 20% of mosquitos tested. More than 50% of mosquitos tested was found in the room without the extracts, but less than 30% was found in the room that released mosquitos. GC/MS analysis detected ${\beta}$-asarone as a main component of the extracts. The commercial asarones (${\alpha}$ and ${\beta}$) showed a repellent activity at 50 mg/L on the filter paper similar to the extracts. CONCLUSION(S): A. gramineus has potential for use as a mosquito repellent since ${\beta}$-asarone, a main component of the plant, exhibited a strong repellent activity against C. pipiens.

식의약소재로서 석창포의 활용성을 조사하고자 석창포 추출물의 모기에 대한 기피효과를 시험한 결과 모기를 방사한 장소에서 추출물에 노출된 공간으로 이동한 모기 마리수 비율은 추출물을 함유하지 않은 공간으로 이동한 모기 마리수 비율에 대비하여 현저하게 낮았다. 또한, 방사한 공간에 남아 있는 모기 마리수 비율은 석창포 추출물을 함유하지 않은 공간으로 이동한 모기 마리수 비율에 비해 낮았다. 이는 모기가 석창포 추출물을 기피하는 경향에서 비롯된 결과임을 의미하였다. 석창포 추출물의 모기기피 활성성분을 조사하고자 추출물을 GC/MS 분석한 결과 주요 성분은 ${\beta}$-asarone으로 밝혀졌다. 시판중인 asarone을 이용하여 기피실험을 실시한 결과 ${\alpha}$-asarone과 ${\beta}$-asarone 모두 기피활성을 보였으며 ${\beta}$-asarone이 ${\alpha}$-asarone에 비해 기피활성이 높았다. 이러한 결과는 석창포 추출물이 친환경적으로 모기를 제어하기 위한 식의약소재로 활용될 수 있음을 의미하였다.

Keywords

References

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