Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Improving Effect of Silk Peptides on the Cognitive Function of Rats with Aging Brain Facilitated by D-Galactose

  • Park, Dong-Sun (College of Veterinary Medicine, Chungbuk National University) ;
  • Lee, Sun-Hee (College of Veterinary Medicine, Chungbuk National University) ;
  • Choi, Young-Jin (College of Veterinary Medicine, Chungbuk National University) ;
  • Bae, Dae-Kwon (College of Veterinary Medicine, Chungbuk National University) ;
  • Yang, Yun-Hui (College of Veterinary Medicine, Chungbuk National University) ;
  • Yang, Go-Eun (College of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Tae-Kyun (College of Veterinary Medicine, Chungbuk National University) ;
  • Yeon, Sung-Ho (Department of Food Science and Technology, Chungbuk National University) ;
  • Hwang, Seock-Yeon (Department of Biomedical Laboratory Science, Daejeon University) ;
  • Joo, Seong-Soo (Division of Marine Molecular Biotechnology, Gangneung-Wonju National University) ;
  • Kim, Yun-Bae (College of Veterinary Medicine, Chungbuk National University)
  • Received : 2010.12.02
  • Accepted : 2011.01.25
  • Published : 2011.04.30
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Abstract

In order to develop silk peptide (SP) preparations possessing cognition-enhancing effect, several candidates were screened through in vitro assays, and their effectiveness was investigated in facilitated brain aging model rats. Incubation of brain acetyl-cholinesterase with SP-PN (1-1,000 ${\mu}g/ml$) led to inhibition of the enzyme activity up to 35%, in contrast to a negligible effect of SP-NN. The expression of choline acetyltransferase (ChAT) mRNA of neural stem cells expressing ChAT gene (F3.ChAT) was increased by 24-hour treatment with 10 and 100 ${\mu}g/ml$ SP-NN (1.35 and 2.20 folds) and SP-PN (2.40 and 1.34 folds). Four-week subcutaneous injections with D-galactose (150 mg/kg) increased activated hippocampal astrocytes to 1.7 folds (a marker of brain injury and aging), decreased acetylcholine concentration in cerebrospinal fluid by 45-50%, and thereby impaired learning and memory function in passive avoidance and water-maze performances. Oral treatment with SP preparations (50 or 300 mg/kg) for 5 weeks from 1 week prior to D-galactose injection exerted recovering activities on acetylcholine depletion and brain injury/aging as well as cognitive deficit induced by D-galactose. The results indicate that SP preparations restore cognitive functions of facilitated brain aging model rats by increasing the release of acetylcholine, in addition to neuroprotective activity.

Keywords

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