Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Protein Expression Analysis in Hematopoietic Stem Cells during Osteopontin-Induced Differentiation of Natural Killer Cells

  • Kim, Mi-Sun (Cell Therapy Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Bae, Kil-Soo (Department of Biological Science, Dong-A University) ;
  • Kim, Hye-Jin (Department of Biological Science, Dong-A University) ;
  • Yoon, Suk-Ran (Cell Therapy Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Oh, Doo-Byung (Aging Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Hwang, Kwang-Woo (Department of Immunology, College of Pharmacy, Chung Ang University) ;
  • Jun, Woo-Jin (Department of Food and Nutrition, Chonnam National University) ;
  • Shim, Sang-In (Department of Agronomy, Gyeongsang National University) ;
  • Kim, Kwang-Dong (Division of Applied Life Science (BK21), Gyeongsang National University) ;
  • Jung, Yong-Woo (Department of Immunology, College of Pharmacy, Korea University Sejong Campus) ;
  • Park, So-Young (Department of Nanobiomedical Science, Dankook University) ;
  • Kwon, Ki-Sun (Aging Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Choi, In-Pyo (Cell Therapy Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Chung, Jin-Woong (Department of Biological Science, Dong-A University)
  • Received : 2010.12.06
  • Accepted : 2011.03.14
  • Published : 2011.04.30
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Abstract

Natural Killer (NK) cells are the lymphocytes that are derived from hematopoietic stem cells, developed in the bone marrow from hematopoietic stem cells (HSC) by sequential acquisition of functional surface receptors, and express the repertoire of inhibitory and activating receptors. Recently, Osteopontin (OPN) has been identified as a critical factor for differentiation of natural killer cells. However, the detailed mechanism of OPN-induced NK differentiation has been still to be elucidated. Here, we determined the signaling pathway and possible receptor for OPN in NK differentiation. OPN induced expression of Bcl-2 and activation of Erk kinase. Inhibition of Erk pathway decreased the effect of OPN on NK differentiation. In addition, the expression of integrin ${\alpha}9$ was significantly increased by OPN during NK differentiation, suggesting the possible role of a major signaling molecule for OPN- induced NK differentiation.

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References

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