Bulletin of the Korean Chemical Society

  • 제32권4호
  • /
  • Pages.1221-1227
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  • 2011
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  • 0253-2964(pISSN)
  • /
  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
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A Simple and Sensitive High Performance Liquid Chromatography-Electrospray Ionization/Mass Spectrometry Method for the Quantification of Ethyl Pyruvate in Rat Plasma

  • Kim, Hyun-Ji (Department of Molecular Science and Technology, Ajou University) ;
  • Kim, Seung-Woo (Department of Anatomy, Center for Advanced Medical Education, School of Medicine, Inha University) ;
  • Lee, Ja-Kyeong (Department of Anatomy, Center for Advanced Medical Education, School of Medicine, Inha University) ;
  • Yoon, Sung-Hwa (Department of Molecular Science and Technology, Ajou University)
  • 투고 : 2010.09.10
  • 심사 : 2011.02.07
  • 발행 : 2011.04.20
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초록

Ethyl pyruvate (EP) is known as a scavenger of reactive oxygen species (ROS) in the body through its role in the donation of diketone groups to metals to form an EP-metal complex. In order to develop a method for the quantification of EP in biological media, a sensitive and specific, high-performance liquid chromatographyelectrospray ionization-mass spectrometry (HPLC-ESI/MS) method is used to determine the EP-alkali metal ion binding species. The analyte was separated on a ZORBOX SB-C8 ($3.5{\mu}m$, $30mm{\times}2.1mm$ I.D.) column and analyzed in selected ion monitoring (SIM) mode with a positive ESI interface using the m/z 255 $[2M + Na]^+$ ion. The method was validated over the concentration range of $0.5-60.0\;{\mu}g$/mL under 1/9 (v/v) of acetonitrile/methanol solvent system with flow rate 0.05 mL/min. The limit of quantification (LOQ) was $0.5{\mu}g$/mL.

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피인용 문헌

  1. Studying the Chemistry of Cationized Triacylglycerols Using Electrospray Ionization Mass Spectrometry and Density Functional Theory Computations vol.25, pp.8, 2014, https://doi.org/10.1007/s13361-014-0917-9